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Literature summary extracted from

  • Kienow, L.; Schneider, K.; Bartsch, M.; Stuible, H.P.; Weng, H.; Miersch, O.; Wasternack, C.; Kombrink, E.
    Jasmonates meet fatty acids: functional analysis of a new acyl-coenzyme A synthetase family from Arabidopsis thaliana (2008), J. Exp. Bot., 59, 403-419.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.2.1.3 additional information the enzyme is inducible by wounding Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 expressed in Escherichia coli strains DH5alpha and M15 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.3 additional information construction of opcl1 mutant, At1g20510-KO-1, plants, which show no induction of jasmonate by wounding in contrast to the wild-type plants, jasmonate profiles in leaves, overview Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.3 additional information
-
 additional information Michaelis-Menten kinetics Arabidopsis thaliana
6.2.1.B3 0.019
-
 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
6.2.1.B3 0.027
-
 12-oxo-phytodienoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
6.2.1.B3 0.036
-
 tetradecanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
6.2.1.B3 0.076
-
 dinor-12-oxo-phytodienoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
6.2.1.B3 0.094
-
 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.3 additional information subcellular localization study Arabidopsis thaliana
-
-
6.2.1.3 peroxisome
-
 Arabidopsis thaliana 5777
-
6.2.1.B3 peroxisome
-
 Arabidopsis thaliana 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.3 Mg2+
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.3 additional information Arabidopsis thaliana the OPC-8:CoA ligase catalyses an essential step in jasmonic acid biosynthesis by initiating the beta-oxidative chain shortening of the carboxylic acid side chain of its precursors, and, in accordance with this function, the protein is localized in peroxisomes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.3 Arabidopsis thaliana
-
 Col-0, Ws-0, gene OPCL1
-
6.2.1.B3 Arabidopsis thaliana Q84P21
-
-
6.2.1.12 Arabidopsis thaliana
-
 ecotype Col-0
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12
-
 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.3 leaf expression levels of OPCL1 Arabidopsis thaliana
-
6.2.1.12 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.B3 12-oxo-phytodienoic acid + CoA + ATP
-
 Arabidopsis thaliana 12-oxo-phytodienoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.B3 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid + CoA + ATP i.e. OPC-6:0 Arabidopsis thaliana 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.B3 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid + CoA + ATP i.e. OPC-8:0 Arabidopsis thaliana 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.3 ATP + 12-oxo-phytodienoic acid + CoA
-
 Arabidopsis thaliana AMP + diphosphate + 12-oxo-phytodienoyl-CoA
-
 ?
6.2.1.3 ATP + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid + CoA
-
 Arabidopsis thaliana AMP + diphosphate + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA
-
 ?
6.2.1.3 ATP + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid + CoA preferred substrate Arabidopsis thaliana AMP + diphosphate + 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA
-
 ?
6.2.1.3 ATP + dinor-12-oxo-phytodienoic acid + CoA
-
 Arabidopsis thaliana AMP + diphosphate + dinor-12-oxo-phytodienoyl-CoA
-
 ?
6.2.1.3 ATP + jasmonic acid + CoA
-
 Arabidopsis thaliana AMP + diphosphate + jasmonyl-CoA
-
 ?
6.2.1.3 ATP + myristic acid + CoA
-
 Arabidopsis thaliana AMP + diphosphate + myristoyl-CoA
-
 ?
6.2.1.B3 decanoic acid + CoA + ATP
-
 Arabidopsis thaliana decanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.B3 dinor-12-oxo-phytodienoic acid + CoA + ATP
-
 Arabidopsis thaliana dinor-12-oxo-phytodienoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.B3 dodecanoic acid + CoA + ATP
-
 Arabidopsis thaliana dodecanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.B3 hexadecanoic acid + CoA + ATP
-
 Arabidopsis thaliana hexadecanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.3 additional information the OPC-8:CoA ligase catalyses an essential step in jasmonic acid biosynthesis by initiating the beta-oxidative chain shortening of the carboxylic acid side chain of its precursors, and, in accordance with this function, the protein is localized in peroxisomes Arabidopsis thaliana ?
-
 ?
6.2.1.3 additional information enzyme family members display activity towards different biosynthetic precursors of jasmonic acid, including 12-oxo-phytodienoic acid, i.e. OPDA, dinor-OPDA, 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid, i.e. OPC-8, and OPC-6, substrate specificity of protein At1g20510 readily converting OPDA, dnOPDA, OPC-8, OPC-6, and myristic acid, OPC-8 is the preferred substrate, overview Arabidopsis thaliana ?
-
 ?
6.2.1.B3 octadecanoic acid + CoA + ATP
-
 Arabidopsis thaliana octadecanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.B3 oleic acid + CoA + ATP
-
 Arabidopsis thaliana oleoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.B3 tetradecanoic acid + CoA + ATP
-
 Arabidopsis thaliana tetradecanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.12 AMP + 3-oxo-2-(2'-[Z]-pentenyl)-cyclopentane-1-butanoic acid + CoA weak substrate Arabidopsis thaliana ?
-
 ?
6.2.1.12 AMP + 3-oxo-2-(2'-[Z]-pentenyl)-cyclopentane-1-hexanoic acid + CoA weak substrate Arabidopsis thaliana ?
-
 ?
6.2.1.12 AMP + dinor-12-oxo-phytodienoic acid + CoA
-
 Arabidopsis thaliana ?
-
 ?
6.2.1.12 ATP + 2-coumarate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + 2-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 3-coumarate + CoA high activity Arabidopsis thaliana AMP + diphosphate + 3-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA high activity Arabidopsis thaliana AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + acetate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA high activity Arabidopsis thaliana AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + decanoate + CoA weak substrate Arabidopsis thaliana AMP + diphosphate + decanoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + heptanoate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + heptanoyl-CoA
-
 ?
6.2.1.12 ATP + hexadecanoate + CoA weak substrate Arabidopsis thaliana AMP + diphosphate + hexadecanoyl-CoA
-
 ?
6.2.1.12 ATP + hexanoate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + hexanoyl-CoA
-
 ?
6.2.1.12 ATP + indole propanoic acid + CoA
-
 Arabidopsis thaliana ?
-
 ?
6.2.1.12 ATP + nonanoate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + nonanoyl-CoA
-
 ?
6.2.1.12 ATP + octadecanoate + CoA weak substrate Arabidopsis thaliana AMP + diphosphate + octadecanoyl-CoA
-
 ?
6.2.1.12 ATP + octanoate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + octanoyl-CoA
-
 ?
6.2.1.12 ATP + oleate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + oleoyl-CoA
-
 ?
6.2.1.12 ATP + propanoate + CoA weak substrate Arabidopsis thaliana AMP + diphosphate + propanoyl-CoA
-
 ?
6.2.1.12 ATP + tetradecanoate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + tetradecanoyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.3 fatty acyl-CoA synthetase
-
 Arabidopsis thaliana
6.2.1.3 More the enzyme belongs to a fatty acyl-CoA synthetase family, which is distinct from the known long-chain fatty acyl-CoA synthetase, LACS, and the 4-coumarate:CoA ligase, 4CL, families, overview Arabidopsis thaliana
6.2.1.3 OPC-8:CoA ligase
-
 Arabidopsis thaliana
6.2.1.3 OPCL1
-
 Arabidopsis thaliana
6.2.1.3 protein At1g20510
-
 Arabidopsis thaliana
6.2.1.12 4-coumarate:CoA ligase
-
 Arabidopsis thaliana
6.2.1.12 4CL2
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.B3 0.96
-
 tetradecanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
6.2.1.B3 1.27
-
 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
6.2.1.B3 1.49
-
 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
6.2.1.B3 1.56
-
 12-oxo-phytodienoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
6.2.1.B3 1.78
-
 dinor-12-oxo-phytodienoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.3 ATP
-
 Arabidopsis thaliana
6.2.1.12 ATP
-
 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
6.2.1.B3 Arabidopsis thaliana expression is activated in response to wounding or infection up

General Information

EC Number General Information Comment Organism
6.2.1.B3 physiological function mutants exhibit 3-oxo-2(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid accumulation and reduced levels of jasmonic acid. Enzyme catalyzes an essential step in jasmonic acid biosynthesis by initiating the beta-oxidative chain shortening of the carboxylic acid side chain of its precursors Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.B3 15.2
-
 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
6.2.1.B3 23.4
-
 dinor-12-oxo-phytodienoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
6.2.1.B3 26.4
-
 tetradecanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
6.2.1.B3 56.2
-
 12-oxo-phytodienoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
6.2.1.B3 65.4
-
 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana