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Literature summary extracted from

  • Kino, K.; Noguchi, A.; Nakazawa, Y.; Yagasaki, M.
    A novel L-amino acid ligase from Bacillus licheniformis (2008), J. Biosci. Bioeng., 106, 313-315.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.49 expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.49 Mg2+
-
 Bacillus licheniformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.49 ATP + L-leucine + an L-amino acid Bacillus licheniformis the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview ADP + phosphate + L-leucyl-L-amino acid
-
 ?
6.3.2.49 ATP + L-leucine + an L-amino acid Bacillus licheniformis NBRC12200 the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview ADP + phosphate + L-leucyl-L-amino acid
-
 ?
6.3.2.49 ATP + L-methionine + an L-amino acid Bacillus licheniformis the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview ADP + phosphate + L-methionyl-L-amino acid
-
 ?
6.3.2.49 ATP + L-methionine + an L-amino acid Bacillus licheniformis NBRC12200 the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview ADP + phosphate + L-methionyl-L-amino acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.49 Bacillus licheniformis
-
 gene BL00235
-
6.3.2.49 Bacillus licheniformis NBRC12200
-
 gene BL00235
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.49 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.49 ATP + L-asparagine + L-methionine
-
 Bacillus licheniformis ADP + phosphate + L-asparaginyl-L-methionine
-
 ?
6.3.2.49 ATP + L-asparagine + L-methionine
-
 Bacillus licheniformis NBRC12200 ADP + phosphate + L-asparaginyl-L-methionine
-
 ?
6.3.2.49 ATP + L-leucine + an L-amino acid the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview Bacillus licheniformis ADP + phosphate + L-leucyl-L-amino acid
-
 ?
6.3.2.49 ATP + L-leucine + an L-amino acid the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview Bacillus licheniformis NBRC12200 ADP + phosphate + L-leucyl-L-amino acid
-
 ?
6.3.2.49 ATP + L-leucine + L-alanine
-
 Bacillus licheniformis ADP + phosphate + L-leucyl-L-alanine
-
 ?
6.3.2.49 ATP + L-leucine + L-alanine
-
 Bacillus licheniformis NBRC12200 ADP + phosphate + L-leucyl-L-alanine
-
 ?
6.3.2.49 ATP + L-methionine + an L-amino acid the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview Bacillus licheniformis ADP + phosphate + L-methionyl-L-amino acid
-
 ?
6.3.2.49 ATP + L-methionine + an L-amino acid the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview Bacillus licheniformis NBRC12200 ADP + phosphate + L-methionyl-L-amino acid
-
 ?
6.3.2.49 ATP + L-methionine + glycine
-
 Bacillus licheniformis ADP + phosphate + L-methionyl-glycine
-
 ?
6.3.2.49 ATP + L-methionine + L-alanine best substrate combination Bacillus licheniformis ADP + phosphate + L-methionyl-L-alanine
-
 ?
6.3.2.49 ATP + L-methionine + L-cysteine
-
 Bacillus licheniformis ADP + phosphate + L-methionyl-L-cysteine
-
 ?
6.3.2.49 ATP + L-methionine + L-leucine
-
 Bacillus licheniformis ADP + phosphate + L-methionyl-L-leucine
-
 ?
6.3.2.49 ATP + L-methionine + L-methionine
-
 Bacillus licheniformis ADP + phosphate + L-methionyl-L-methionine
-
 ?
6.3.2.49 ATP + L-serine + L-methionine
-
 Bacillus licheniformis ADP + phosphate + L-seryl-L-methionine
-
 ?
6.3.2.49 ATP + L-threonine + L-methionine
-
 Bacillus licheniformis ADP + phosphate + L-threonyl-L-methionine
-
 ?
6.3.2.49 additional information the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is very strict, overview Bacillus licheniformis ?
-
 ?
6.3.2.49 additional information the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is very strict, overview Bacillus licheniformis NBRC12200 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.49 BL00235
-
 Bacillus licheniformis
6.3.2.49 L-amino acid ligase
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.49 37
-
-
 Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.49 9
-
-
 Bacillus licheniformis

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.49 ATP
-
 Bacillus licheniformis