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Literature summary extracted from
- A novel flavin adenine dinucleotide (FAD) containing D-lactate dehydrogenase from the thermoacidophilic crenarchaeota Sulfolobus tokodaii strain 7: purification, characterization and expression in Escherichia coli (2008), J. Biosci. Bioeng., 106, 16-21.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.99.6 | expression in Escherichia coli | Sulfurisphaera tokodaii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.6 | 4-chloromercuribenzoate | 1 mM, 62% loss of activity | Sulfurisphaera tokodaii |  |
| 1.1.99.6 | CoCl2 | 1 mM, 78% loss of activity | Sulfurisphaera tokodaii | |
| 1.1.99.6 | CuCl2 | 1 mM, complete loss of activity | Sulfurisphaera tokodaii | |
| 1.1.99.6 | HgCl2 | 1 mM, 93% loss of activity | Sulfurisphaera tokodaii | |
| 1.1.99.6 | MnCl2 | 1 mM, complete loss of activity | Sulfurisphaera tokodaii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.6 | 0.091 | - |
(R)-lactate | pH 8.5, 60°C, cosubstrate: 2,6-dichlorophenolindophenol | Sulfurisphaera tokodaii | |
| 1.1.99.6 | 0.156 | - |
(R)-lactate | pH 8.5, 60°C, cosubstrate: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | Sulfurisphaera tokodaii | |
| 1.1.99.6 | 0.247 | - |
oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | pH 8.5, 60°C, cosubstrate: (R)-lactate | Sulfurisphaera tokodaii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.6 | 48000 | - |
2 * 48000, SDS-PAGE | Sulfurisphaera tokodaii |
| 1.1.99.6 | 49715 | - |
2 * 49715, calculated from sequence | Sulfurisphaera tokodaii |
| 1.1.99.6 | 93000 | - |
non-denaturing PAGE | Sulfurisphaera tokodaii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.6 | Sulfurisphaera tokodaii | Q974L0 | - |
- |
| 1.1.99.6 | Sulfurisphaera tokodaii 7 | Q974L0 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.6 | - |
Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.6 | (R)-2-hydroxybutyrate + oxidized 2,6-dichlorophenolindophenol | 97% of the activity compared to (R)-lactate | Sulfurisphaera tokodaii | 2-oxobutyrate + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.6 | (R)-2-hydroxybutyrate + oxidized 2,6-dichlorophenolindophenol | 97% of the activity compared to (R)-lactate | Sulfurisphaera tokodaii 7 | 2-oxobutyrate + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.6 | (R)-lactate + ferricyanide | ferricyanide is the preferred electron acceptor | Sulfurisphaera tokodaii | pyruvate + ferrocyanide | - |
? | |
| 1.1.99.6 | (R)-lactate + ferricyanide | ferricyanide is the preferred electron acceptor | Sulfurisphaera tokodaii 7 | pyruvate + ferrocyanide | - |
? | |
| 1.1.99.6 | (R)-lactate + oxidized 2,6-dichlorophenolindophenol | activity is 59% compared to the activity with ferricyanide | Sulfurisphaera tokodaii | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.6 | (R)-lactate + oxidized 2,6-dichlorophenolindophenol | activity is 59% compared to the activity with ferricyanide | Sulfurisphaera tokodaii 7 | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.6 | (R)-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | activity is 29% compared to the activity with ferricyanide | Sulfurisphaera tokodaii | pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | - |
? | |
| 1.1.99.6 | (R)-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | activity is 29% compared to the activity with ferricyanide | Sulfurisphaera tokodaii 7 | pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | - |
? | |
| 1.1.99.6 | additional information | L-lactate, glycolate, L-tartrate, citrate, glyoxylate, 2-hydroxyisobutyrate, D-glycerate and D-2-phosphoglycerate are inert as electron donors. Benzyl viologen and methylene blue are inert as electron acceptors | Sulfurisphaera tokodaii | ? | - |
? | |
| 1.1.99.6 | additional information | L-lactate, glycolate, L-tartrate, citrate, glyoxylate, 2-hydroxyisobutyrate, D-glycerate and D-2-phosphoglycerate are inert as electron donors. Benzyl viologen and methylene blue are inert as electron acceptors | Sulfurisphaera tokodaii 7 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.6 | homodimer | 2 * 48000, SDS-PAGE | Sulfurisphaera tokodaii |
| 1.1.99.6 | homodimer | 2 * 49715, calculated from sequence | Sulfurisphaera tokodaii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.99.6 | dye-linked D-lactate dehydrogenase | - |
Sulfurisphaera tokodaii |
| 1.1.99.6 | ST0649 | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.6 | 60 | - |
assay at | Sulfurisphaera tokodaii |
| 1.1.99.6 | 80 | - |
- |
Sulfurisphaera tokodaii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.6 | 80 | - |
10 min, enzyme retains full activity | Sulfurisphaera tokodaii |
| 1.1.99.6 | 85 | - |
10 min, more than 60% loss of activity | Sulfurisphaera tokodaii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.6 | 8.5 | - |
60°C | Sulfurisphaera tokodaii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.6 | 6.5 | 9 | pH 6.5: about 50% of maximal activity, pH 9.0: about 50% of maximal activity | Sulfurisphaera tokodaii |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.6 | 6.5 | 10 | 50°C, 30 min, enzyme retains full activity | Sulfurisphaera tokodaii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.6 | FAD | prosthetic group | Sulfurisphaera tokodaii | |
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