Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yao, P.; Zhou, X.L.; He, R.; Xue, M.Q.; Zheng, Y.G.; Wang, Y.F.; Wang, E.D.
    Unique residues crucial for optimal editing in yeast cytoplasmic Leucyl-tRNA synthetase are revealed by using a novel knockout yeast strain (2008), J. Biol. Chem., 283, 22591-22600.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens
6.1.1.4 expressed in Escherichia coli as a His-tagged fusion protein Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.4 D399K mutant is resitant to inhibitor 5-fluoro-2,1-benzoxaborol-1(3H)-ol but more sensitive to norvaline inhibition Homo sapiens
6.1.1.4 D418R mutant is significantly resistant to inhibitor AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Growth rate is moderately inhibited in medium containing a large excess of norvaline and reduced leucine Saccharomyces cerevisiae
6.1.1.4 D419A mutant is significantly resistant to inhibitor AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Mutant D419A shows mischarging capacity with Ile. Growth rate is severly inhibited in medium containing a large excess of norvaline and reduced leucine Saccharomyces cerevisiae
6.1.1.4 DELTA270-530 deletion of the CP1 domain shows that the mutant is not able to rescue LeuRS knock-out strain Saccharomyces cerevisiae
6.1.1.4 DELTA314-319 deletion of the T-rich region shows that the mutant is able to rescue LeuRS knock-out strain with a grwoth rate similar to wild-type Saccharomyces cerevisiae
6.1.1.4 K404Y mutant is significantly resistant to inhibitor AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Growth rate is moderately inhibited in medium containing a large excess of norvaline and reduced leucine Saccharomyces cerevisiae
6.1.1.4 additional information deletion of the leuS gene is lethal. Human cytoplasmic LeuRS can rescue the knock-out strain but not Escherichia coli LeuRS. LeuRS mutant strains T319, D419A, K404Y, S416D, D418R, T347A or T410A are also able to rescue the null mutant Saccharomyces cerevisiae
6.1.1.4 additional information growth rate of wild-type strain in media containing a large excess of noncognate amino acid norvaline and reduced leucine is reduced to 35% Saccharomyces cerevisiae
6.1.1.4 S416D mutant is significantly resistant to inhibitor AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Growth rate is moderately inhibited in medium containing a large excess of norvaline and reduced leucine Saccharomyces cerevisiae
6.1.1.4 T31E mutant is significantly resistant to inhibitor AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Mutant T319A shows mischarging capacity with Ile. Growth rate is severly inhibited in medium containing a large excess of norvaline and reduced leucine Saccharomyces cerevisiae
6.1.1.4 T347A mutant is inhibited by AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Growth rate is moderately inhibited in medium containing a large excess of norvaline and reduced leucine Saccharomyces cerevisiae
6.1.1.4 T410A mutant is inhibited by AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Growth rate is moderately inhibited in medium containing a large excess of norvaline and reduced leucine Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.4 5-fluoro-2,1-benzoxaborol-1(3H)-ol AN-2690, antibiotic which specifically targets the editing active site of LeuRS Homo sapiens
6.1.1.4 5-fluoro-2,1-benzoxaborol-1(3H)-ol AN-2690, antibiotic which specifically targets the editing active site of LeuRS Saccharomyces cerevisiae
6.1.1.4 norvaline
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.0041
-
 L-leucine
-
 Saccharomyces cerevisiae
6.1.1.4 0.167
-
 norvaline
-
 Saccharomyces cerevisiae
6.1.1.4 8.713
-
 L-isoleucine
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.4 cytoplasm
-
 Homo sapiens 5737
-
6.1.1.4 cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Homo sapiens
-
-
-
6.1.1.4 Saccharomyces cerevisiae P26637
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4 using Ni-NTA chromatography Homo sapiens
6.1.1.4 using Ni-NTA chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-isoleucine + tRNALeu
-
 Homo sapiens AMP + diphosphate + L-isoleucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-isoleucine + tRNALeu
-
 Saccharomyces cerevisiae AMP + diphosphate + L-isoleucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Saccharomyces cerevisiae AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-norvaline + tRNALeu
-
 Saccharomyces cerevisiae AMP + diphosphate + L-norvalyl-tRNALeu
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.4 HcleuRS
-
 Homo sapiens
6.1.1.4 Leucyl-tRNA synthetase
-
 Homo sapiens
6.1.1.4 leucyl—tRNA synthetase
-
 Saccharomyces cerevisiae
6.1.1.4 LeuRS
-
 Homo sapiens
6.1.1.4 LeuRS
-
 Saccharomyces cerevisiae
6.1.1.4 ycLeuRS
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.4 30
-
 assay at Homo sapiens
6.1.1.4 30
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 10.2
-
 L-isoleucine
-
 Saccharomyces cerevisiae
6.1.1.4 13.5
-
 norvaline
-
 Saccharomyces cerevisiae
6.1.1.4 37.5
-
 L-leucine
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 7.5
-
 assay at Homo sapiens
6.1.1.4 7.5
-
 assay at Saccharomyces cerevisiae