BRENDA - Enzyme Database

Human UMP-CMP kinase 2, a novel nucleoside monophosphate kinase localized in mitochondria

Xu, Y.; Johansson, M.; Karlsson, A.; J. Biol. Chem. 283, 1563-1571 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.14
UMP-CMPK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His6-tagged mutant UMP-CMPK2DELTA21, lacking the mitochondrial targeting sequence, in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, expression of GFP-tagged wild-type and E448G mutant enzymes in HeLa cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.4.14
E448G
site-directed mutagenesis
Homo sapiens
2.7.4.14
additional information
construction of a UMP-CMPK2DELTA21 mutant lacking the mitochondrial targeting sequence
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.1
-
dUMP
pH 8.0, 37°C, wild-type enzyme
Homo sapiens
2.7.4.14
1.31
-
dCMP
pH 8.0, 37°C, wild-type enzyme
Homo sapiens
2.7.4.14
3.09
-
CMP
pH 8.0, 37°C, wild-type enzyme
Homo sapiens
2.7.4.14
6.3
-
UMP
pH 8.0, 37°C, wild-type enzyme
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.14
mitochondrion
UMP-CMPK2 contains an N-terminal mitochondrial targeting sequence
Homo sapiens
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.14
additional information
Homo sapiens
UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis, it is highly expressed in leukemia cells, in bone marrow, and is tightly correlated with macrophage activation and inflammatory response
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.14
Homo sapiens
Q5EBM0
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.14
recombinant His6-tagged mutant UMP-CMPK2DELTA21 from Spodoptera frugiperda Sf9 cells by affinity chromatography
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.14
bone marrow
-
Homo sapiens
-
2.7.4.14
HeLa cell
-
Homo sapiens
-
2.7.4.14
K-562 cell
chronic myelogenous leukemia cell line
Homo sapiens
-
2.7.4.14
leukemia cell
high expression
Homo sapiens
-
2.7.4.14
macrophage
-
Homo sapiens
-
2.7.4.14
MOLT-4 cell
lymphoblastic leukemia cell line
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + CMP
low activity
693063
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dCMP
high activity
693063
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dUMP
best substrate
693063
Homo sapiens
ADP + dUDP
-
-
-
?
2.7.4.14
ATP + UMP
low activity
693063
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
additional information
UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis, it is highly expressed in leukemia cells, in bone marrow, and is tightly correlated with macrophage activation and inflammatory response
693063
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.4.14
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.14
8
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.14
ATP
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.14
UMP-CMPK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His6-tagged mutant UMP-CMPK2DELTA21, lacking the mitochondrial targeting sequence, in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, expression of GFP-tagged wild-type and E448G mutant enzymes in HeLa cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.14
ATP
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.4.14
E448G
site-directed mutagenesis
Homo sapiens
2.7.4.14
additional information
construction of a UMP-CMPK2DELTA21 mutant lacking the mitochondrial targeting sequence
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.1
-
dUMP
pH 8.0, 37°C, wild-type enzyme
Homo sapiens
2.7.4.14
1.31
-
dCMP
pH 8.0, 37°C, wild-type enzyme
Homo sapiens
2.7.4.14
3.09
-
CMP
pH 8.0, 37°C, wild-type enzyme
Homo sapiens
2.7.4.14
6.3
-
UMP
pH 8.0, 37°C, wild-type enzyme
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.14
mitochondrion
UMP-CMPK2 contains an N-terminal mitochondrial targeting sequence
Homo sapiens
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.14
additional information
Homo sapiens
UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis, it is highly expressed in leukemia cells, in bone marrow, and is tightly correlated with macrophage activation and inflammatory response
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.14
recombinant His6-tagged mutant UMP-CMPK2DELTA21 from Spodoptera frugiperda Sf9 cells by affinity chromatography
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.14
bone marrow
-
Homo sapiens
-
2.7.4.14
HeLa cell
-
Homo sapiens
-
2.7.4.14
K-562 cell
chronic myelogenous leukemia cell line
Homo sapiens
-
2.7.4.14
leukemia cell
high expression
Homo sapiens
-
2.7.4.14
macrophage
-
Homo sapiens
-
2.7.4.14
MOLT-4 cell
lymphoblastic leukemia cell line
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + CMP
low activity
693063
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dCMP
high activity
693063
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dUMP
best substrate
693063
Homo sapiens
ADP + dUDP
-
-
-
?
2.7.4.14
ATP + UMP
low activity
693063
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
additional information
UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis, it is highly expressed in leukemia cells, in bone marrow, and is tightly correlated with macrophage activation and inflammatory response
693063
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.4.14
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.14
8
-
assay at
Homo sapiens