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Literature summary extracted from

  • Bagautdinov, B.; Matsuura, Y.; Bagautdinova, S.; Kunishima, N.
    Protein biotinylation visualized by a complex structure of biotin protein ligase with a substrate (2008), J. Biol. Chem., 283, 14739-14750.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.15 purified recombinant enzyme, complexed with biotinyl-5'-AMP, biotin, adenosine, or mutant biotin carboxyl carrier protein PhBCCPN76, X-ray diffraction structure determination at 2.0-2.7 A resolution, analysis, and modelling. The non-hydrolyzable intermediate analogue biotinol-5'-AMP does not provide analyzable complex crystals Pyrococcus horikoshii

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.15 R48A/K111A site-directed mutagenesis, comparison of the nucleotide binding mode in the double mutant to that in the wild-type enzyme, the active site loop Gly45-Trp53 is disordered in the mutant. Interface charge distribution in the double mutant complex, overview Pyrococcus horikoshii
6.3.4.15 R48K site-directed mutagenesis, comparison of the nucleotide binding mode in the mutant to that in the wild-type enzyme, the active site loop Gly45-Trp53 is disordered in the mutant Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.15 Mg2+
-
 Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Pyrococcus horikoshii
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Pyrococcus horikoshii OT-3
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Pyrococcus horikoshii
-
 strain OT3
-
6.3.4.15 Pyrococcus horikoshii OT-3
-
 strain OT3
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Pyrococcus horikoshii AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Pyrococcus horikoshii OT-3 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 additional information structural features influencing the reaction mechanism, overview Pyrococcus horikoshii ?
-
 ?
6.3.4.15 additional information structural features influencing the reaction mechanism, overview Pyrococcus horikoshii OT-3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.15 More acetyl-CoA carboxylase biotinyl domain structure analysis, overview Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
6.3.4.15 biotin protein ligase
-
 Pyrococcus horikoshii
6.3.4.15 BPL
-
 Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.15 ATP
-
 Pyrococcus horikoshii