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Literature summary extracted from

  • Neuhierl, B.; Thanbichler, M.; Lottspeich, F.; Böck, A.
    A family of S-methylmethionine-dependent thiol/selenol methyltransferases. Role in selenium tolerance and evolutionary relation (1999), J. Biol. Chem., 274, 5407-5414.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.10
-
 Escherichia coli
2.1.1.280
-
 Escherichia coli
2.1.1.280
-
 Astragalus bisulcatus
2.1.1.280 cloned into plasmid pT7-7 and overexpression in Escherichia coli BL21(DE3) cells Escherichia coli
2.1.1.280 pUKE vector used for cloning of cDNA sequence coding for the SeCys-methyltransferase from Astragalus bisulcatus in Escherichia coli Astragalus bisulcatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.10 L-methionine product inhibition above 0.5 mM Escherichia coli
2.1.1.10 S-adenosyl-L-methionine product inhibition above 0.25 mM Escherichia coli
2.1.1.280 L-methionine product inhibition above 0.5 mM Escherichia coli
2.1.1.280 S-adenosyl-L-methionine product inhibition above 0.25 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.10 0.045
-
 L-homocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Escherichia coli
2.1.1.280 0.043
-
 DL-selenohomocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Escherichia coli
2.1.1.280 0.043
-
 DL-selenohomocysteine app. Km-value with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
2.1.1.280 0.045
-
 L-homocysteine app. Km-value with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
2.1.1.280 0.35
-
 DL-selenohomocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Astragalus bisulcatus
2.1.1.280 0.35
-
 DL-selenohomocysteine app. Km-value with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C Astragalus bisulcatus
2.1.1.280 0.59
-
 L-selenocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Escherichia coli
2.1.1.280 0.59
-
 L-selenocysteine app. Km-valuen with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
2.1.1.280 0.7
-
 L-selenocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Astragalus bisulcatus
2.1.1.280 0.7
-
 L-selenocysteine app. Km-value with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C Astragalus bisulcatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.280 chloroplast
-
 Astragalus bisulcatus 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.10 32500
-
 1 * 32500, calculated from sequence Escherichia coli
2.1.1.10 34000
-
 gel filtration Escherichia coli
2.1.1.10 35000
-
 1 * 35000, SDS-PAGE Escherichia coli
2.1.1.280 22500
-
 1 * 22500, calculated from sequence Escherichia coli
2.1.1.280 33500
-
 calculated from gene sequence Escherichia coli
2.1.1.280 34000
-
 gel filtration Escherichia coli
2.1.1.280 35000
-
 SDS-PAGE Escherichia coli
2.1.1.280 36700
-
 calculated from nucleotide sequence Astragalus bisulcatus
2.1.1.280 36700
-
 x * 36700, calculated from sequence Astragalus bisulcatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.10 S-methyl-L-methionine + L-homocysteine Escherichia coli
-
 2 L-methionine
-
 ?
2.1.1.280 S-methyl-L-methionine + L-selenocysteine Escherichia coli
-
 L-methionine + Se-methyl-L-selenocysteine
-
 ?
2.1.1.280 S-methyl-L-methionine + L-selenocysteine Escherichia coli S-methylmethionine is the physiological methyl group donor L-methionine + Se-methyl-L-selenocysteine
-
 ?
2.1.1.280 S-methyl-L-methionine + L-selenocysteine Astragalus bisulcatus S-methylmethionine is the physiological methyl group donor. The enzyme is involved in detoxification of selenium in selenium-accumulating plants L-methionine + Se-methyl-L-selenocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.10 Escherichia coli Q47690
-
-
2.1.1.280 Astragalus bisulcatus
-
-
-
2.1.1.280 Astragalus bisulcatus P56707
-
-
2.1.1.280 Escherichia coli Q47690
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.10 recombinant enzyme Escherichia coli
2.1.1.280
-
 Astragalus bisulcatus
2.1.1.280 by ammonium sulfate precipitation, hydrophobic interaction chromatography and anion exchange chromatography with Q-Sepharose Astragalus bisulcatus
2.1.1.280 by ammonium sulfate precipitation, hydrophobic interaction chromatography and anion exchange chromatography with Q-Sepharose Escherichia coli
2.1.1.280 recombinant enzyme Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.280 cell culture cell cultures contain the maximal level of selenocysteine methyltransferase under conditions of exponential growth. Stationary phase cells possess a much lower amount Astragalus bisulcatus
-
2.1.1.280 cotyledon of 4-8-week-old seedlings Astragalus bisulcatus
-
2.1.1.280 cotyledon immunoblotting analysis Astragalus bisulcatus
-
2.1.1.280 hypocotyl of 4-8-week-old seedlings Astragalus bisulcatus
-
2.1.1.280 hypocotyl immunoblotting analysis Astragalus bisulcatus
-
2.1.1.280 leaf primary of 4-8-week-old seedlings Astragalus bisulcatus
-
2.1.1.280 primary leaf immunoblotting analysis Astragalus bisulcatus
-
2.1.1.280 root of 4-8-week-old seedlings Astragalus bisulcatus
-
2.1.1.280 root immunoblotting analysis Astragalus bisulcatus
-
2.1.1.280 seedling
-
 Astragalus bisulcatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.280 additional information
-
 with L-S-methylmethionine, maximal conversion is 100%, pH 6.0, 30°C Astragalus bisulcatus
2.1.1.280 0.016
-
 with [methyl-14C]S-adenosyl-L-methionine, maximal conversion is 10%, pH 6.0, 30°C Astragalus bisulcatus
2.1.1.280 0.68
-
 with S-adenosyl-L-methionine, maximal conversion is 20-25%, pH 6.0, 30°C Astragalus bisulcatus
2.1.1.280 1.5
-
 with DL-S-methylmethionine, maximal conversion is 50%, pH 6.0, 30°C Astragalus bisulcatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.10 additional information the enzyme also catalyzes the reaction of selenocysteine methyltransferase Escherichia coli ?
-
 ?
2.1.1.10 S-adenosyl-L-methionine + L-homocysteine
-
 Escherichia coli S-adenosyl-L-homocysteine + L-methionine
-
 ?
2.1.1.10 S-methyl-L-methionine + L-homocysteine
-
 Escherichia coli 2 L-methionine
-
 ?
2.1.1.280 additional information the enzyme also catalyzes the reaction of EC 2.1.1.10 Escherichia coli ?
-
 ?
2.1.1.280 additional information no activity with trimethylsulfonium and glycine betaine as methyl donor Astragalus bisulcatus ?
-
 ?
2.1.1.280 additional information no activity with trimethylsulfonium and glycine betaine as methyl donor Escherichia coli ?
-
 ?
2.1.1.280 S-adenosyl-L-methionine + 4-thiobutyric acid 0.014% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli S-adenosyl-L-homocysteine + 4-methylthiobutyric acid
-
 ?
2.1.1.280 S-adenosyl-L-methionine + D-homocysteine 16% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli ?
-
 ?
2.1.1.280 S-adenosyl-L-methionine + DL-homocysteine 93% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli ?
-
 ?
2.1.1.280 S-adenosyl-L-methionine + DL-selenohomocysteine 213% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli ?
-
 ?
2.1.1.280 S-adenosyl-L-methionine + L-cysteine less than 0.003% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli S-adenosyl-L-homocysteine + S-methyl-L-cysteine
-
 ?
2.1.1.280 S-adenosyl-L-methionine + L-homocysteine
-
 Escherichia coli ?
-
 ?
2.1.1.280 S-adenosyl-L-methionine + L-homoserine less than 0.003% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli ?
-
 ?
2.1.1.280 S-adenosyl-L-methionine + L-selenocysteine
-
 Astragalus bisulcatus S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
-
 ?
2.1.1.280 S-adenosyl-L-methionine + L-selenocysteine
-
 Escherichia coli S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
-
 ?
2.1.1.280 S-adenosyl-L-methionine + L-selenocysteine S-adenosyl-L-methionine at intracellular concentrations cannot serve as an effective substrate for the enzyme Astragalus bisulcatus S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
-
 ?
2.1.1.280 S-adenosyl-L-methionine + L-selenocysteine 0.027% relative activity compared to activity with L-homocysteine set at 100% Escherichia coli S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
-
 ?
2.1.1.280 S-adenosyl-L-methionine + L-selenohomocysteine
-
 Escherichia coli ?
-
 ?
2.1.1.280 S-methyl-L-methionine + DL-selenohomocysteine
-
 Astragalus bisulcatus ?
-
 ?
2.1.1.280 S-methyl-L-methionine + L-selenocysteine
-
 Astragalus bisulcatus L-methionine + Se-methyl-L-selenocysteine
-
 ?
2.1.1.280 S-methyl-L-methionine + L-selenocysteine
-
 Escherichia coli L-methionine + Se-methyl-L-selenocysteine
-
 ?
2.1.1.280 S-methyl-L-methionine + L-selenocysteine S-methylmethionine is the physiological methyl group donor Escherichia coli L-methionine + Se-methyl-L-selenocysteine
-
 ?
2.1.1.280 S-methyl-L-methionine + L-selenocysteine S-methylmethionine is the physiological methyl group donor. The enzyme is involved in detoxification of selenium in selenium-accumulating plants Astragalus bisulcatus L-methionine + Se-methyl-L-selenocysteine
-
 ?
2.1.1.280 S-methyl-L-methionine + L-selenocysteine activity with S-methyl-L-methionine is higher than with S-adenosyl-L-methionine Escherichia coli L-methionine + Se-methyl-L-selenocysteine
-
 ?
2.1.1.280 S-methyl-L-methionine + L-selenocysteine activity with S-methyl-L-methionine is higher than with S-adenosyl-L-methionine Astragalus bisulcatus L-methionine + Se-methyl-L-selenocysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.10 monomer 1 * 35000, SDS-PAGE Escherichia coli
2.1.1.10 monomer 1 * 32500, calculated from sequence Escherichia coli
2.1.1.280 ? x * 36700, calculated from sequence Astragalus bisulcatus
2.1.1.280 monomer 1 * 35000, SDS-PAGE Escherichia coli
2.1.1.280 monomer 1 * 22500, calculated from sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.1.1.10 YagD
-
 Escherichia coli
2.1.1.10 YagD protein
-
 Escherichia coli
2.1.1.280 Hcy methyltransferase
-
 Escherichia coli
2.1.1.280 SeCys-methyltransferase
-
 Astragalus bisulcatus
2.1.1.280 SeCys-MT
-
 Astragalus bisulcatus
2.1.1.280 selenocysteine methyltransferase
-
 Astragalus bisulcatus
2.1.1.280 smtA
-
 Astragalus bisulcatus
2.1.1.280 YagD
-
 Escherichia coli
2.1.1.280 YagD protein
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.10 0.38
-
 L-homocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Escherichia coli
2.1.1.280 0.00017
-
 L-selenocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Escherichia coli
2.1.1.280 0.00017
-
 L-selenocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
2.1.1.280 0.0062
-
 L-selenocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Astragalus bisulcatus
2.1.1.280 0.0062
-
 L-selenocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C Astragalus bisulcatus
2.1.1.280 0.0074
-
 DL-selenohomocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Astragalus bisulcatus
2.1.1.280 0.0074
-
 DL-selenohomocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C Astragalus bisulcatus
2.1.1.280 0.38
-
 L-homocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
2.1.1.280 0.74
-
 DL-selenohomocysteine pH 6, 30°C, cosubstrate: S-adenosyl-L-methionine Escherichia coli
2.1.1.280 0.74
-
 DL-selenohomocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli

General Information

EC Number General Information Comment Organism
2.1.1.280 metabolism detoxification of selenium-containing compounds Astragalus bisulcatus
2.1.1.280 metabolism detoxification of selenium-containing compounds Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.280 0.00029
-
 L-selenocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
2.1.1.280 0.0089
-
 L-selenocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C Astragalus bisulcatus
2.1.1.280 0.021
-
 DL-selenohomocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.0, 30°C Astragalus bisulcatus
2.1.1.280 8.4
-
 L-homocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli
2.1.1.280 17
-
 DL-selenohomocysteine with [methyl-14C]S-adenosyl-L-methionine, pH 6.5, 30°C Escherichia coli