EC Number | Application | Comment | Organism |
---|---|---|---|
1.2.1.72 | degradation | coupling of a transketolase reaction (using Leishmania mexicana transketolase) that converts D-fructose 6-phosphate to D-erythrose 4-phosphate, which can then be converted to 4-phosphate D-erythronate using E4PD, whereby D-ribose 5-phosphate and D-glyceraldehyde 3-phosphate can both be used as ketol acceptor substrates in the reaction | Escherichia coli |
2.2.1.1 | analysis | coupling of a transketolase reaction that converts D-fructose 6-phosphate to D-erythrose 4-phosphate, which can then be converted to 4-phosphate D-erythronate using erythrose-4-phosphate dehydrogenase, a reaction that reduces NAD+ to NADH and can be easily followed spectrophotometrically | Leishmania mexicana |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.2.1.1 | - |
Leishmania mexicana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.1 | fructose 6-phosphate | at concentrations higher than 10 mM fructose 6-phosphate causes an inhibition of the enzyme | Leishmania mexicana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.1 | 0.61 | - |
fructose 6-phosphate | - |
Leishmania mexicana |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.1 | Mg2+ | - |
Leishmania mexicana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.72 | Escherichia coli | P0A9B6 | - |
- |
2.2.1.1 | Leishmania mexicana | Q8MPM3 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.2.1.1 | 1.8 | - |
using 10 mM D-glyceraldehyde 3-phosphate | Leishmania mexicana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.72 | D-erythrose 4-phosphate + NAD+ | production of E4P can be coupled to the NADH generating E4PD activity which enables measurement of the transketolase reaction involving F6P as donor substrate | Escherichia coli | 4-phosphoerythronate + NADH | - |
? | |
1.2.1.72 | D-ribose 5-phosphate + NAD+ | D-Ribose 5-phosphate can be used as ketol acceptor substrate in the reaction although D-ribose 5-phosphate is also a substrate for the coupling enzyme | Escherichia coli | ? + NADH | - |
? | |
1.2.1.72 | additional information | fructose 6-phosphate, glycerol 6-phosphate and glyceraldehyde 3-phosphate can not be used as substrate by E4PD when added up to 50 mM | Escherichia coli | ? | - |
? | |
2.2.1.1 | D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate | - |
Leishmania mexicana | D-erythrose 4-phosphate + D-xylulose 5-phosphate | - |
? | |
2.2.1.1 | D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Leishmania mexicana | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.72 | E4PD | - |
Escherichia coli |
2.2.1.1 | TKT | - |
Leishmania mexicana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.72 | NAD+ | - |
Escherichia coli | |
2.2.1.1 | thiamine diphosphate | - |
Leishmania mexicana |