Literature summary extracted from

Lee, H.J.; Yoon, Y.J.; Jang, D.S.; Kim, C.; Cha, H.J.; Hong, B.H.; Choi, K.Y.; Lee, H.C.
15N NMR relaxation studies of Y14F mutant of ketosteroid isomerase: the influence of mutation on backbone mobility (2008), J. Biochem., 144, 159-166.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
5.3.3.1	study on backbone dynamics in free enzyme and its complex with a steroid analogue, 19-nortestosterone hemisuccinate. Mutation Y14F induces a substantial decrease in the order parameters in free enzyme, indicating that the backbone structures become significantly mobile by mutation, while the chemical shift analysis indicates that the structural perturbations are more profound than those of wild-type upon 19-nortestosterone hemisuccinate binding. In the 19-nortestosterone hemisuccinate complexed mutant, the key active site residues including Tyr14, Asp38 and Asp99 or the regions around them remain flexible with significantly reduced S2 values, whereas the S2 values for many of the residues in the mutant enzyme become even greater than those of wild-type	Comamonas testosteroni

Protein Variants

EC Number	Protein Variants	Comment	Organism
5.3.3.1	Y14F	study on backbone dynamics in free enzyme and its complex with a steroid analogue, 19-nortestosterone hemisuccinate. Mutation induces a substantial decrease in the order parameters in free enzyme, indicating that the backbone structures become significantly mobile by mutation, while the chemical shift analysis indicates that the structural perturbations are more profound than those of wild-type upon 19-nortestosterone hemisuccinate binding. In the 19-nortestosterone hemisuccinate complexed mutant, the key active site residues including Tyr14, Asp38 and Asp99 or the regions around them remain flexible with significantly reduced S2 values, whereas the S2 values for many of the residues in the mutant enzyme become even greater than those of wild-type	Comamonas testosteroni

Organism

EC Number	Organism	UniProt	Comment	Textmining
5.3.3.1	Comamonas testosteroni	-	-	-

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Release 2023.1 (January 2023)