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Literature summary extracted from

  • Westman, E.L.; Preston, A.; Field, R.A.; Lam, J.S.
    Biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharides of both Pseudomonas aeruginosa and Bordetella pertussis occurs via an identical scheme despite different gene clusters (2008), J. Bacteriol., 190, 6060-6069.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.201 gene wbpD, expression and subcloning of His6-tagged WbpD in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa
2.6.1.98 gene wbpE, expression of wild-type and mutants in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa
2.6.1.98 gene wlbC, i.e. wbpE, in Escherichia coli strain BL21(DE3), expression in wbpE knockout mutant of Pseudomonas aeruginosa PAO1 serotype O5 Bordetella pertussis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.201 additional information construction of a gene wbpD knockout strain, functional complementation by expression of WbpB from Bordetella pertussis, overview Pseudomonas aeruginosa
2.6.1.98 additional information construction of a nonpolar knockout of each of wbpA, wbpB, wbpE, wbpD, and wbpI genes. Expression of Bordetella pertussis genes wbpO1629 and wbpO3150 complements a wbpA knockout of Pseudomonas aeruginosa. B-band LPS production is restored to Pseudomonas aeruginosa knockout mutants when the relevant Bordetella pertussis genes are supplied in trans Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.201 acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate Pseudomonas aeruginosa
-
 CoA + UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate
-
 ?
2.3.1.201 additional information Pseudomonas aeruginosa the enzyme is involved in the biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharide 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid ?
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate Pseudomonas aeruginosa WbpE/WlbC product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 r
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate Bordetella pertussis WbpE/WlbC product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.201 Pseudomonas aeruginosa
-
 gene wbpD
-
2.6.1.98 Bordetella pertussis
-
 gene wlbC
-
2.6.1.98 Pseudomonas aeruginosa
-
 serogroup O2, gene wlbC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.201 recombinant His6-tagged WbpD from Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.201 acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate
-
 Pseudomonas aeruginosa CoA + UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate
-
 ?
2.3.1.201 additional information the enzyme is involved in the biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharide 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid Pseudomonas aeruginosa ?
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate WbpE/WlbC product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate Pseudomonas aeruginosa UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 r
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate WbpE/WlbC product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate Bordetella pertussis UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.3.1.201 WbpD
-
 Pseudomonas aeruginosa
2.6.1.98 WbpE
-
 Pseudomonas aeruginosa
2.6.1.98 WbpE
-
 Bordetella pertussis
2.6.1.98 WlbC
-
 Pseudomonas aeruginosa
2.6.1.98 WlbC
-
 Bordetella pertussis

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.201 acetyl-CoA
-
 Pseudomonas aeruginosa
2.6.1.98 pyridoxal 5'-phosphate
-
 Pseudomonas aeruginosa
2.6.1.98 pyridoxal 5'-phosphate
-
 Bordetella pertussis

General Information

EC Number General Information Comment Organism
2.6.1.98 malfunction B-band LPS production is restored to Pseudomonas aeruginosa knockout mutants when the relevant Bordetella pertussis genes are supplied in trans Pseudomonas aeruginosa
2.6.1.98 metabolism the biosynthetic pathway of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid production as part of the B-band LPS production requires the five enzymes WbpA, WbpB, WbpE, WbpD, and WbpI Pseudomonas aeruginosa
2.6.1.98 metabolism the biosynthetic pathway of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid production as part of the B-band LPS production requires the five enzymes WbpA, WbpB, WbpE, WbpD, and WbpI Bordetella pertussis
2.6.1.98 additional information Bordetella pertussis genes wbpO1629 and wbpO3150 complement a wbpA knockout of Pseudomonas aeruginosa. B-band LPS production is restored to Pseudomonas aeruginosa knockout mutants when the relevant Bordetella pertussis genes are supplied in trans Bordetella pertussis