Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Park, M.O.; Mizutani, T.; Jones, P.R.
    Glyceraldehyde-3-phosphate ferredoxin oxidoreductase from Methanococcus maripaludis (2007), J. Bacteriol., 189, 7281-7289.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.6 isopropyl-beta-D-thiogalactopyranoside 1 mM induces cultures at the exponential phase of growth Methanococcus maripaludis
1.2.7.6 L-cysteine reversibly activates GAPOR purified from M9/Mo+ cultures Methanococcus maripaludis
1.2.7.6 Sodium molybdate
-
 Methanococcus maripaludis

Application

EC Number Application Comment Organism
1.2.7.6 degradation the major physiological role of GAPOR in Methanococcus maripaludis most likely involves only nonoptimal growth conditions Methanococcus maripaludis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.7.6 pET46-Ek/LIC-GAPOR vector expressed in Escherichia coli strains BL21(DE3) or Rosetta-gami 2(DE3) Methanococcus maripaludis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.6 ATP 0.001-0.01 mM completely inhibits Methanococcus maripaludis
1.2.7.6 D-Glyceraldehyde-3-phosphate recombinant GAPOR is posttranscriptionally regulated as it exhibits pronounced and irreversible substrate inhibition. Inhibition is independent of benzyl viologen Methanococcus maripaludis
1.2.7.6 additional information addition of 0.001 mM AMP, NADH, or NADPH has no effect on GAPOR activity Methanococcus maripaludis
1.2.7.6 O2 reversibly inactivates GAPOR purified from M9/Mo+ cultures. GAPOR activity of crude lysates is irreversibly inactivated Methanococcus maripaludis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.6 Fe2+
-
 Methanococcus maripaludis
1.2.7.6 S2- Mo-GAPOR expressed in Escherichia coli strain BL21(DE3) is estimated to contain 6.1 g-atoms of acid-labile sulfide per mol of protein Methanococcus maripaludis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.6 70000
-
 gel filtration Methanococcus maripaludis

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.6 Methanococcus maripaludis A4FXX8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.6 to more than 75% homogeneity, by gel filtration Methanococcus maripaludis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.6 1.8
-
 maximum specific activity of protein for Mo-GAPOR expressed in Escherichia coli strain BL21(DE3) Methanococcus maripaludis
1.2.7.6 120
-
 Mo-GAPOR purified from Escherichia coli strain Rosetta-gami 2(DE3) Methanococcus maripaludis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.6 D-glyceraldehyde-3-phosphate + H2O + oxidized benzyl viologen only GAPOR catalyzes D-glyceraldehyde-3-phosphate oxidation, catalysis under autotrophic conditions Methanococcus maripaludis 3-phospho-D-glycerate + H+ + reduced benzyl viologen
-
 ir
1.2.7.6 D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin
-
 Methanococcus maripaludis 3-phospho-D-glycerate + H+ + reduced ferredoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.6 monomer gel filtration Methanococcus maripaludis

Synonyms

EC Number Synonyms Comment Organism
1.2.7.6 GAPOR
-
 Methanococcus maripaludis
1.2.7.6 glyceraldehyde-3-phosphate ferredoxin oxidoreductase
-
 Methanococcus maripaludis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.6 Ferredoxin
-
 Methanococcus maripaludis
1.2.7.6 molybdopterin content of Mo-GAPOR expressed in Escherichia coli strain BL21(DE3) is estimated to be 0.35 g-atoms per mol of protein Methanococcus maripaludis
1.2.7.6 additional information GAPOR obtained from cells grown in medium containing tungsten and molybdenum or in medium without added tungsten and molybdenum do not display any activity Methanococcus maripaludis