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Literature summary extracted from

  • Olguin, L.F.; Askew, S.E.; ODonoghue, A.C.; Hollfelder, F.
    Efficient catalytic promiscuity in an enzyme superfamily: An arylsulfatase shows a rate acceleration of 10(13) for phosphate monoester hydrolysis (2008), J. Am. Chem. Soc., 130, 16547-16555.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.6.1 C51A mutant with strongly reduced activity Pseudomonas aeruginosa
3.1.6.1 C51S mutant with strongly reduced activity Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.1 4-nitrophenyl phosphate
-
Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.1 0.00025
-
4-nitrophenyl sulfate mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
3.1.6.1 0.00029
-
4-nitrophenyl sulfate wild type enzyme, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
3.1.6.1 0.0004
-
4-nitrophenyl sulfate mutant enzyme C51A, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
3.1.6.1 0.0291
-
4-nitrophenyl phosphate wild type enzyme, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
3.1.6.1 0.0752
-
4-nitrophenyl phosphate mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.1 60000
-
SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.1 Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.1 4-nitrophenyl phosphate + H2O
-
Pseudomonas aeruginosa 4-nitrophenol + phosphate
-
?
3.1.6.1 4-nitrophenyl sulfate + H2O
-
Pseudomonas aeruginosa 4-nitrophenol + sulfate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.6.1 PAS
-
Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.6.1 0.0000095
-
4-nitrophenyl phosphate mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
3.1.6.1 0.000061
-
4-nitrophenyl sulfate mutant enzyme C51A, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
3.1.6.1 0.0054
-
4-nitrophenyl sulfate mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
3.1.6.1 0.023
-
4-nitrophenyl phosphate wild type enzyme, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
3.1.6.1 14.2
-
4-nitrophenyl sulfate wild type enzyme, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.6.1 0.0292
-
4-nitrophenyl phosphate wild type enzyme, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa