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Literature summary extracted from

  • Gopishetty, B.; Zhu, J.; Rajan, R.; Sobczak, A.J.; Wnuk, S.F.; Bell, C.E.; Pei, D.
    Probing the catalytic mechanism of S-ribosylhomocysteinase (LuxS) with catalytic intermediates and substrate analogues (2009), J. Am. Chem. Soc., 131, 1243-1250.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.21 C83A mutant shows lower activity than the wild type enzyme Vibrio harveyi
4.4.1.21 E57A mutant shows lower activity than the wild type enzyme Vibrio harveyi
4.4.1.21 E57D mutant shows lower activity than the wild type enzyme Vibrio harveyi
4.4.1.21 H11Q mutant shows lower activity than the wild type enzyme Vibrio harveyi
4.4.1.21 R39M mutant shows lower activity than the wild type enzyme Vibrio harveyi
4.4.1.21 S6A mutant shows lower activity than the wild type enzyme Vibrio harveyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.21 S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine
-
Bacillus subtilis
4.4.1.21 S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine
-
Escherichia coli
4.4.1.21 S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine
-
Vibrio harveyi
4.4.1.21 S-[3-fluoro-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine
-
Bacillus subtilis
4.4.1.21 S-[3-fluoro-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine
-
Escherichia coli
4.4.1.21 S-[3-fluoro-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine
-
Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.21 0.0023
-
S-ribosylhomocysteine wild type enzyme Bacillus subtilis
4.4.1.21 0.0036
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid wild type enzyme Bacillus subtilis
4.4.1.21 0.027
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid wild type enzyme Escherichia coli
4.4.1.21 0.027
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid wild type enzyme Vibrio harveyi
4.4.1.21 0.037
-
S-ribosylhomocysteine wild type enzyme Escherichia coli
4.4.1.21 0.037
-
S-ribosylhomocysteine wild type enzyme Vibrio harveyi
4.4.1.21 0.041
-
S-ribosylhomocysteine mutant enzyme S6A Vibrio harveyi
4.4.1.21 0.041
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme S6A Vibrio harveyi
4.4.1.21 0.098
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme E57A Vibrio harveyi
4.4.1.21 0.15
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme R39M Vibrio harveyi
4.4.1.21 0.52
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme H11Q Vibrio harveyi
4.4.1.21 0.7
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme C83A Vibrio harveyi
4.4.1.21 0.73
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme E57D Vibrio harveyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.21 Fe2+ LuxS is a metalloenzyme containing a tetrahedrally coordinated Fe2+ ion in its active site Bacillus subtilis
4.4.1.21 Fe2+ LuxS is a metalloenzyme containing a tetrahedrally coordinated Fe2+ ion in its active site Escherichia coli
4.4.1.21 Fe2+ LuxS is a metalloenzyme containing a tetrahedrally coordinated Fe2+ ion in its active site Vibrio harveyi

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.21 Bacillus subtilis
-
-
-
4.4.1.21 Escherichia coli
-
-
-
4.4.1.21 Vibrio harveyi
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.21 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid
-
Bacillus subtilis L-homocysteine + ?
-
?
4.4.1.21 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid
-
Escherichia coli L-homocysteine + ?
-
?
4.4.1.21 (2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid
-
Vibrio harveyi L-homocysteine + ?
-
?
4.4.1.21 S-ribosylhomocysteine
-
Bacillus subtilis L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
?
4.4.1.21 S-ribosylhomocysteine
-
Escherichia coli L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
?
4.4.1.21 S-ribosylhomocysteine
-
Vibrio harveyi L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.21 LuxS
-
Bacillus subtilis
4.4.1.21 LuxS
-
Escherichia coli
4.4.1.21 LuxS
-
Vibrio harveyi
4.4.1.21 S-ribosylhomocysteinase
-
Bacillus subtilis
4.4.1.21 S-ribosylhomocysteinase
-
Escherichia coli
4.4.1.21 S-ribosylhomocysteinase
-
Vibrio harveyi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.21 0.012
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme E57A Vibrio harveyi
4.4.1.21 0.016
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme R39M Vibrio harveyi
4.4.1.21 0.022
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme C83A Vibrio harveyi
4.4.1.21 0.024
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme H11Q Vibrio harveyi
4.4.1.21 0.026
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme E57D Vibrio harveyi
4.4.1.21 0.027
-
S-ribosylhomocysteine mutant enzyme S6A Vibrio harveyi
4.4.1.21 0.03
-
S-ribosylhomocysteine wild type enzyme Bacillus subtilis
4.4.1.21 0.038
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid wild type enzyme Bacillus subtilis
4.4.1.21 0.14
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid mutant enzyme S6A Vibrio harveyi
4.4.1.21 0.4
-
S-ribosylhomocysteine wild type enzyme Escherichia coli
4.4.1.21 0.4
-
S-ribosylhomocysteine wild type enzyme Vibrio harveyi
4.4.1.21 0.4
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid wild type enzyme Escherichia coli
4.4.1.21 0.64
-
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid wild type enzyme Vibrio harveyi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.4.1.21 0.0079
-
S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine wild type enzyme Bacillus subtilis
4.4.1.21 0.0106
-
S-[3-fluoro-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine wild type enzyme Bacillus subtilis
4.4.1.21 0.047
-
S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine wild type enzyme Escherichia coli
4.4.1.21 0.047
-
S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine wild type enzyme Vibrio harveyi