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Literature summary extracted from

  • Rothman, S.C.; Johnston, J.B.; Lee, S.; Walker, J.R.; Poulter, C.D.
    Type II isopentenyl diphosphate isomerase: irreversible inactivation by covalent modification of flavin (2008), J. Am. Chem. Soc., 130, 4906-4913.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.2 expression in Escherichia coli Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.3.2 (Z)-3-difluoromethyl-2-buten-1-yl diphosphate reversible Thermus thermophilus
5.3.3.2 (Z)-3-fluoromethyl-2-buten-1-yl diphosphate reversible Thermus thermophilus
5.3.3.2 3,4-oxido-3-methyl-1-butyl diphosphate inactivation through formation of covalent adducts with the reduced flavin, modification of the isoalloxazine ring at position N5 Thermus thermophilus
5.3.3.2 3-(Fluoromethyl)-3-buten-1-yl diphosphate inactivation through formation of covalent adducts with the reduced flavin Thermus thermophilus
5.3.3.2 3-cyclopropyl-3-buten-1-yl diphosphate reversible Thermus thermophilus
5.3.3.2 3-methylene-4-penten-1-yl diphosphate inactivation through formation of covalent adducts with the reduced flavin Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.2 Thermus thermophilus
-
 isoform IDI-2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.2 (Z)-3-(difluoromethyl)-2-buten-1-yl diphosphate about 2% of the activity with isopenteyl diphosphate Thermus thermophilus ?
-
 ?
5.3.3.2 (Z)-3-(fluoromethyl)-2-buten-1-yl diphosphate about 2% of the activity with isopenteyl diphosphate Thermus thermophilus ?
-
 ?
5.3.3.2 3-(fluoromethyl)-3-buten-1-yl diphosphate competition between isomerization and alkylation of the flavin cofactor Thermus thermophilus ?
-
 ?
5.3.3.2 3-methylene-4-penten-1-yl diphosphate competition between isomerization and alkylation of the flavin cofactor Thermus thermophilus ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.3.2 0.0074
-
 3-(Fluoromethyl)-3-buten-1-yl diphosphate pH 7.0, 37°C Thermus thermophilus
5.3.3.2 0.008
-
 3-methylene-4-penten-1-yl diphosphate pH 7.0, 37°C Thermus thermophilus
5.3.3.2 0.012
-
 (Z)-3-fluoromethyl-2-buten-1-yl diphosphate pH 7.0, 37°C Thermus thermophilus
5.3.3.2 0.0486
-
 3,4-oxido-3-methyl-1-butyl diphosphate pH 7.0, 37°C Thermus thermophilus
5.3.3.2 0.054
-
 3-cyclopropyl-3-buten-1-yl diphosphate pH 7.0, 37°C Thermus thermophilus
5.3.3.2 0.383
-
 (Z)-3-difluoromethyl-2-buten-1-yl diphosphate pH 7.0, 37°C Thermus thermophilus