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Literature summary extracted from

  • Slavoff, S.A.; Chen, I.; Choi, Y.; Ting, A.Y.
    Expanding the substrate tolerance of biotin ligase through exploration of enzymes from diverse species (2008), J. Am. Chem. Soc., 130, 1160-1162.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.15 overexpression in Escherichia coli Bacillus subtilis
6.3.4.15 overexpression in Escherichia coli Homo sapiens
6.3.4.15 overexpression in Escherichia coli Saccharomyces cerevisiae
6.3.4.15 overexpression in Escherichia coli Giardia intestinalis
6.3.4.15 overexpression in Escherichia coli Leuconostoc mesenteroides
6.3.4.15 overexpression in Escherichia coli Trypanosoma cruzi
6.3.4.15 overexpression in Escherichia coli Methanocaldococcus jannaschii
6.3.4.15 overexpression in Escherichia coli Cutibacterium acnes
6.3.4.15 overexpression in Escherichia coli Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.15 Mg2+
-
 Bacillus subtilis
6.3.4.15 Mg2+
-
 Escherichia coli
6.3.4.15 Mg2+
-
 Homo sapiens
6.3.4.15 Mg2+
-
 Saccharomyces cerevisiae
6.3.4.15 Mg2+
-
 Giardia intestinalis
6.3.4.15 Mg2+
-
 Leuconostoc mesenteroides
6.3.4.15 Mg2+
-
 Trypanosoma cruzi
6.3.4.15 Mg2+
-
 Methanocaldococcus jannaschii
6.3.4.15 Mg2+
-
 Cutibacterium acnes
6.3.4.15 Mg2+
-
 Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Bacillus subtilis
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Escherichia coli
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Homo sapiens
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Saccharomyces cerevisiae
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Giardia intestinalis
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Leuconostoc mesenteroides
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Trypanosoma cruzi
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Methanocaldococcus jannaschii
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Cutibacterium acnes
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Pyrococcus horikoshii
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Bacillus subtilis
-
-
-
6.3.4.15 Cutibacterium acnes
-
-
-
6.3.4.15 Escherichia coli
-
 gene birA
-
6.3.4.15 Giardia intestinalis
-
-
-
6.3.4.15 Homo sapiens
-
-
-
6.3.4.15 Leuconostoc mesenteroides
-
-
-
6.3.4.15 Methanocaldococcus jannaschii
-
-
-
6.3.4.15 Pyrococcus horikoshii
-
-
-
6.3.4.15 Saccharomyces cerevisiae
-
-
-
6.3.4.15 Trypanosoma cruzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.15 recombinant enzyme from Escherichia coli Bacillus subtilis
6.3.4.15 recombinant enzyme from Escherichia coli Saccharomyces cerevisiae
6.3.4.15 recombinant enzyme from Escherichia coli Giardia intestinalis
6.3.4.15 recombinant enzyme from Escherichia coli Leuconostoc mesenteroides
6.3.4.15 recombinant enzyme from Escherichia coli Trypanosoma cruzi
6.3.4.15 recombinant enzyme from Escherichia coli Methanocaldococcus jannaschii
6.3.4.15 recombinant enzyme from Escherichia coli Cutibacterium acnes
6.3.4.15 recombinant enzyme from Escherichia coli Pyrococcus horikoshii
6.3.4.15 recombinant enzyme from Escherichia coli, the human enzyme degrades significantly during isolation Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Bacillus subtilis AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Escherichia coli AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Homo sapiens AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Saccharomyces cerevisiae AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Giardia intestinalis AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Leuconostoc mesenteroides AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Trypanosoma cruzi AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Methanocaldococcus jannaschii AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Cutibacterium acnes AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Pyrococcus horikoshii AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + protein p67
-
 Bacillus subtilis AMP + diphosphate + biotinyl-protein p67
-
 ?
6.3.4.15 ATP + biotin + protein p67
-
 Escherichia coli AMP + diphosphate + biotinyl-protein p67
-
 ?
6.3.4.15 ATP + biotin + protein p67
-
 Homo sapiens AMP + diphosphate + biotinyl-protein p67
-
 ?
6.3.4.15 ATP + biotin + protein p67
-
 Saccharomyces cerevisiae AMP + diphosphate + biotinyl-protein p67
-
 ?
6.3.4.15 ATP + biotin + protein p67
-
 Leuconostoc mesenteroides AMP + diphosphate + biotinyl-protein p67
-
 ?
6.3.4.15 ATP + biotin + protein p67
-
 Trypanosoma cruzi AMP + diphosphate + biotinyl-protein p67
-
 ?
6.3.4.15 ATP + biotin + protein p67
-
 Methanocaldococcus jannaschii AMP + diphosphate + biotinyl-protein p67
-
 ?
6.3.4.15 ATP + biotin + protein p67
-
 Cutibacterium acnes AMP + diphosphate + biotinyl-protein p67
-
 ?
6.3.4.15 ATP + biotin + protein p67
-
 Pyrococcus horikoshii AMP + diphosphate + biotinyl-protein p67
-
 ?
6.3.4.15 ATP + cis-propargyl biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Pyrococcus horikoshii AMP + diphosphate + cis-propargyl bioti-containing [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + cis-propargyl biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Saccharomyces cerevisiae AMP + diphosphate + cis-propargyl biotin-containing [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + desthiobiotin azide + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Pyrococcus horikoshii AMP + diphosphate + desthiobiotin azide-containing [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 additional information no activity with protein p67 Giardia intestinalis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.15 biotin ligase
-
 Bacillus subtilis
6.3.4.15 biotin ligase
-
 Escherichia coli
6.3.4.15 biotin ligase
-
 Homo sapiens
6.3.4.15 biotin ligase
-
 Saccharomyces cerevisiae
6.3.4.15 biotin ligase
-
 Giardia intestinalis
6.3.4.15 biotin ligase
-
 Leuconostoc mesenteroides
6.3.4.15 biotin ligase
-
 Trypanosoma cruzi
6.3.4.15 biotin ligase
-
 Methanocaldococcus jannaschii
6.3.4.15 biotin ligase
-
 Cutibacterium acnes
6.3.4.15 biotin ligase
-
 Pyrococcus horikoshii
6.3.4.15 BirA
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.15 30
-
 assay at Bacillus subtilis
6.3.4.15 30
-
 assay at Escherichia coli
6.3.4.15 30
-
 assay at Homo sapiens
6.3.4.15 30
-
 assay at Saccharomyces cerevisiae
6.3.4.15 30
-
 assay at Giardia intestinalis
6.3.4.15 30
-
 assay at Leuconostoc mesenteroides
6.3.4.15 30
-
 assay at Trypanosoma cruzi
6.3.4.15 30
-
 assay at Methanocaldococcus jannaschii
6.3.4.15 30
-
 assay at Cutibacterium acnes
6.3.4.15 30
-
 assay at Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.15 ATP
-
 Bacillus subtilis
6.3.4.15 ATP
-
 Escherichia coli
6.3.4.15 ATP
-
 Homo sapiens
6.3.4.15 ATP
-
 Saccharomyces cerevisiae
6.3.4.15 ATP
-
 Giardia intestinalis
6.3.4.15 ATP
-
 Leuconostoc mesenteroides
6.3.4.15 ATP
-
 Trypanosoma cruzi
6.3.4.15 ATP
-
 Methanocaldococcus jannaschii
6.3.4.15 ATP
-
 Cutibacterium acnes
6.3.4.15 ATP
-
 Pyrococcus horikoshii
6.3.4.15 biotin
-
 Giardia intestinalis
6.3.4.15 biotin specific for Pyrococcus horikoshii
6.3.4.15 biotin highly specific for Bacillus subtilis
6.3.4.15 biotin highly specific for Escherichia coli
6.3.4.15 biotin highly specific for Homo sapiens
6.3.4.15 biotin highly specific for Saccharomyces cerevisiae
6.3.4.15 biotin highly specific for Leuconostoc mesenteroides
6.3.4.15 biotin highly specific for Trypanosoma cruzi
6.3.4.15 biotin highly specific for Methanocaldococcus jannaschii
6.3.4.15 biotin highly specific for Cutibacterium acnes
6.3.4.15 cis-propargyl biotin can substitute for biotin Saccharomyces cerevisiae
6.3.4.15 cis-propargyl biotin can substitute for biotin Pyrococcus horikoshii
6.3.4.15 desthiobiotin azide can substitute for biotin Pyrococcus horikoshii
6.3.4.15 additional information no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid Bacillus subtilis
6.3.4.15 additional information no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid Escherichia coli
6.3.4.15 additional information no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid Homo sapiens
6.3.4.15 additional information no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid Leuconostoc mesenteroides
6.3.4.15 additional information no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid Trypanosoma cruzi
6.3.4.15 additional information no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid Methanocaldococcus jannaschii
6.3.4.15 additional information no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid Cutibacterium acnes
6.3.4.15 additional information no activity with biotin analogues desthiobiotin azide, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid Saccharomyces cerevisiae
6.3.4.15 additional information no activity with biotin analogues trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid Pyrococcus horikoshii