Literature summary extracted from
Elleuche, S.; Poeggeler, S.
A cyanase is transcriptionally regulated by arginine and involved in cyanate decomposition in Sordaria macrospora (2008), Fungal Genet. Biol., 45, 1458-1469.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
4.2.1.104 |
bicarbonate |
is required for the catalytic activity of the Sordaria macrospora cyanase |
Sordaria macrospora |
|
4.2.1.104 |
Cyanate |
- |
Sordaria macrospora |
|
4.2.1.104 |
additional information |
cyn1 transcript levels are strongly down-regulated by the addition of arginine and up-regulated by cyanate. The addition of both, cyanate and arginine, results in a loss of regulation. It seems that arginine and cyanate have an antagonistic effect on the expression of the Sordaria macrospora cyn1 |
Sordaria macrospora |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.2.1.104 |
expression in Escherichia coli strain M15 |
Sordaria macrospora |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.2.1.104 |
E107A |
site-directed mutagenesis, soluble, no activity |
Sordaria macrospora |
4.2.1.104 |
E107D |
site-directed mutagenesis, soluble, no activity |
Sordaria macrospora |
4.2.1.104 |
additional information |
a delta cyn1 knockout in Sordaria macrospora is totally devoid of cyanase activity and shows an increased sensitivity to exogenously supplied cyanate in an arginine-depleted medium, defects in ascospore germination, but no other obvious morphological phenotype |
Sordaria macrospora |
4.2.1.104 |
additional information |
Arg104, Glu107 and Ser130 are essential for enzyme activity of the fungal cyanase, irrespectively of substitution by Alanine or another amino-acid |
Sordaria macrospora |
4.2.1.104 |
R104A |
site-directed mutagenesis, soluble, no activity |
Sordaria macrospora |
4.2.1.104 |
R104L |
site-directed mutagenesis, soluble, no activity |
Sordaria macrospora |
4.2.1.104 |
S130A |
site-directed mutagenesis, soluble, no activity |
Sordaria macrospora |
4.2.1.104 |
S130T |
site-directed mutagenesis, soluble, no activity |
Sordaria macrospora |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.2.1.104 |
Cyanate |
partial auto-inhibition at high concentrations of potassium cyanate |
Sordaria macrospora |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.2.1.104 |
0.58 |
- |
Cyanate |
bicarbonate concentration 5 mM |
Sordaria macrospora |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.2.1.104 |
19000 |
- |
SDS-PAGE, monomer calculated: 18100 + His-tag: 600 |
Sordaria macrospora |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.2.1.104 |
cyanate + HCO3- + 2 H+ |
Sordaria macrospora |
- |
NH3 + 2 CO2 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.1.104 |
Sordaria macrospora |
D1ZHN6 |
- |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
4.2.1.104 |
mycelium |
- |
Sordaria macrospora |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.1.104 |
cyanate + HCO3- + 2 H+ |
- |
Sordaria macrospora |
NH3 + 2 CO2 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.2.1.104 |
monomer |
19000 recombinant enzyme + His-tag |
Sordaria macrospora |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.2.1.104 |
cyanase |
- |
Sordaria macrospora |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
4.2.1.104 |
27 |
80 |
quite stable even at high temperatures |
Sordaria macrospora |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
4.2.1.104 |
27 |
80 |
quite stable even at high temperatures |
Sordaria macrospora |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.2.1.104 |
7.6 |
- |
potassium phosphate buffer (50 mM), 27°C, 10 min |
Sordaria macrospora |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
4.2.1.104 |
4.4 |
8.8 |
using potassium phosphate buffer (50 mM) the enzyme retains more than 50% activity over a wide pH range after incubation at 27°C for 10 min |
Sordaria macrospora |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
4.2.1.104 |
4.4 |
8.8 |
using potassium phosphate buffer (50 mM) the enzyme retains more than 50% activity over a wide pH range after incubation at 27°C for 10 min |
Sordaria macrospora |