BRENDA - Enzyme Database show

MJ0400 from Methanocaldococcus jannaschii exhibits fructose-1,6-bisphosphate aldolase activity

Samland, A.K.; Wang, M.; Sprenger, G.A.; FEMS Microbiol. Lett. 281, 36-41 (2008)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.2.13
additional information
citrate or phosphoenolpyruvate show no effect on the FBP activity of MJ0400-His6
Methanocaldococcus jannaschii
Cloned(Commentary)
EC Number
Commentary
Organism
2.2.1.10
gene Mj0400, DNA and amino acid sequence determination and analysis, overexpression of the His6-tagged enzyme in Escherichia coli strain Rosetta2(DE3)pLysS as mainly insoluble protein
Methanocaldococcus jannaschii
4.1.2.13
cloning, overexpression of C-terminally His-tagged MJ0400 enzyme in Escherichia coli Rosetta2(DE3)pLysS cells
Methanocaldococcus jannaschii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.2.1.10
4-O-phosphonato-D-erythrose
competitive inhibition of aldolase activity
Methanocaldococcus jannaschii
2.2.1.10
glycylglycine
slightly inhibiting as buffer at 50 mM
Methanocaldococcus jannaschii
4.1.2.13
D-erythrose 4-phosphate
competitive
Methanocaldococcus jannaschii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2.1.10
additional information
-
additional information
MJ0400-His6 exhibits Michaelis-Menten kinetics in the aldolase reaction
Methanocaldococcus jannaschii
4.1.2.13
0.43
-
D-fructose-1,6-bisphosphate
at 50°C
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.2.1.10
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
Methanocaldococcus jannaschii
-
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + 2,3-dioxopropyl phosphate
-
-
?
4.1.2.13
D-fructose-1,6-bisphosphate
Methanocaldococcus jannaschii
-
glyceraldehyde-3-phosphate + dihydroxyacetone phosphate
-
-
r
4.1.2.13
additional information
Methanocaldococcus jannaschii
no activity of MJ0400-His6 is measured with 10 mM fructose-1-phosphate as a substrate. MJ0400 also acts as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, this indicates that MJ0400 is involved in both the carbon metabolism and the shikimate pathway in Methanocaldococcus jannaschii
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.2.1.10
Methanocaldococcus jannaschii
Q57843
gene mj0400
-
4.1.2.13
Methanocaldococcus jannaschii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.2.1.10
soluble fraction of recombinant His6-tagged MJ0400 from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography to over 97% purity
Methanocaldococcus jannaschii
4.1.2.13
-
Methanocaldococcus jannaschii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.2.1.10
cell culture
optimal growth temperature is 85°C
Methanocaldococcus jannaschii
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.2.1.10
0.033
-
purified recombinant enzyme, pH 7.5, 50°C, buffer Tris-HCl, HEPES/NaOH, or Na-phosphate
Methanocaldococcus jannaschii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.2.1.10
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
-
692386
Methanocaldococcus jannaschii
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + 2,3-dioxopropyl phosphate
-
-
-
?
2.2.1.10
additional information
MJ0400 acts as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, catalyzing the reaction of L-aspartate semialdehyde and 6-deoxy-5-ketofructose-1-phosphate to 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid, but the recombinant His6-tagged MJ0400 also catalyzes the cleavage of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate, exhibiting fructose-1,6-bisphosphate aldolase, FBP aldolase, activity, EC 4.1.2.13, or the transaldolase reaction with D-fructose-6-phosphate and D-erythose-4-phosphate as substrates. The enzyme shows high substrate specificity for fructose-6-phosphate
692386
Methanocaldococcus jannaschii
?
-
-
-
-
2.2.1.10
pyruvate + D-erythrose 4-phosphate
-
692386
Methanocaldococcus jannaschii
?
-
-
-
?
4.1.2.13
D-fructose-1,6-bisphosphate
-
692386
Methanocaldococcus jannaschii
glyceraldehyde-3-phosphate + dihydroxyacetone phosphate
-
-
-
r
4.1.2.13
additional information
no activity of MJ0400-His6 is measured with 10 mM fructose-1-phosphate as a substrate. MJ0400 also acts as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, this indicates that MJ0400 is involved in both the carbon metabolism and the shikimate pathway in Methanocaldococcus jannaschii
692386
Methanocaldococcus jannaschii
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.2.1.10
50
-
assay at
Methanocaldococcus jannaschii
4.1.2.13
50
-
assay at, temperature optimum for the specific acitivity of MJ0400-His6
Methanocaldococcus jannaschii
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.2.1.10
25
85
10% activity at 25°C, higher activity at 50°C, optimal activity probably at or above the optimal growth temperature of 85°C
Methanocaldococcus jannaschii
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.2.1.10
80
-
10 mg/ml purified recombinant His6-tagged enzyme, 50 mM Tris-HCl, pH 7.5, 1 mM DTT, half-life is 37 h
Methanocaldococcus jannaschii
2.2.1.10
100
-
10 mg/ml purified recombinant His6-tagged enzyme, 50 mM Tris-HCl, pH 7.5, 1 mM DTT, half-life is 1 h
Methanocaldococcus jannaschii
4.1.2.13
additional information
-
displays heat stability with a half-life of 1 h at 100°C
Methanocaldococcus jannaschii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.2.1.10
7.5
-
assay at
Methanocaldococcus jannaschii
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.2.1.10
0.38
-
4-O-phosphonato-D-erythrose
recombinant enzyme, pH 7.5, 50°C
Methanocaldococcus jannaschii
4.1.2.13
0.38
-
D-erythrose 4-phosphate
-
Methanocaldococcus jannaschii
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.2.13
additional information
citrate or phosphoenolpyruvate show no effect on the FBP activity of MJ0400-His6
Methanocaldococcus jannaschii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.2.1.10
gene Mj0400, DNA and amino acid sequence determination and analysis, overexpression of the His6-tagged enzyme in Escherichia coli strain Rosetta2(DE3)pLysS as mainly insoluble protein
Methanocaldococcus jannaschii
4.1.2.13
cloning, overexpression of C-terminally His-tagged MJ0400 enzyme in Escherichia coli Rosetta2(DE3)pLysS cells
Methanocaldococcus jannaschii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.2.1.10
4-O-phosphonato-D-erythrose
competitive inhibition of aldolase activity
Methanocaldococcus jannaschii
2.2.1.10
glycylglycine
slightly inhibiting as buffer at 50 mM
Methanocaldococcus jannaschii
4.1.2.13
D-erythrose 4-phosphate
competitive
Methanocaldococcus jannaschii
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.2.1.10
0.38
-
4-O-phosphonato-D-erythrose
recombinant enzyme, pH 7.5, 50°C
Methanocaldococcus jannaschii
4.1.2.13
0.38
-
D-erythrose 4-phosphate
-
Methanocaldococcus jannaschii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2.1.10
additional information
-
additional information
MJ0400-His6 exhibits Michaelis-Menten kinetics in the aldolase reaction
Methanocaldococcus jannaschii
4.1.2.13
0.43
-
D-fructose-1,6-bisphosphate
at 50°C
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.2.1.10
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
Methanocaldococcus jannaschii
-
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + 2,3-dioxopropyl phosphate
-
-
?
4.1.2.13
D-fructose-1,6-bisphosphate
Methanocaldococcus jannaschii
-
glyceraldehyde-3-phosphate + dihydroxyacetone phosphate
-
-
r
4.1.2.13
additional information
Methanocaldococcus jannaschii
no activity of MJ0400-His6 is measured with 10 mM fructose-1-phosphate as a substrate. MJ0400 also acts as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, this indicates that MJ0400 is involved in both the carbon metabolism and the shikimate pathway in Methanocaldococcus jannaschii
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.2.1.10
soluble fraction of recombinant His6-tagged MJ0400 from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography to over 97% purity
Methanocaldococcus jannaschii
4.1.2.13
-
Methanocaldococcus jannaschii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.2.1.10
cell culture
optimal growth temperature is 85°C
Methanocaldococcus jannaschii
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.2.1.10
0.033
-
purified recombinant enzyme, pH 7.5, 50°C, buffer Tris-HCl, HEPES/NaOH, or Na-phosphate
Methanocaldococcus jannaschii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.2.1.10
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
-
692386
Methanocaldococcus jannaschii
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + 2,3-dioxopropyl phosphate
-
-
-
?
2.2.1.10
additional information
MJ0400 acts as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, catalyzing the reaction of L-aspartate semialdehyde and 6-deoxy-5-ketofructose-1-phosphate to 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid, but the recombinant His6-tagged MJ0400 also catalyzes the cleavage of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate, exhibiting fructose-1,6-bisphosphate aldolase, FBP aldolase, activity, EC 4.1.2.13, or the transaldolase reaction with D-fructose-6-phosphate and D-erythose-4-phosphate as substrates. The enzyme shows high substrate specificity for fructose-6-phosphate
692386
Methanocaldococcus jannaschii
?
-
-
-
-
2.2.1.10
pyruvate + D-erythrose 4-phosphate
-
692386
Methanocaldococcus jannaschii
?
-
-
-
?
4.1.2.13
D-fructose-1,6-bisphosphate
-
692386
Methanocaldococcus jannaschii
glyceraldehyde-3-phosphate + dihydroxyacetone phosphate
-
-
-
r
4.1.2.13
additional information
no activity of MJ0400-His6 is measured with 10 mM fructose-1-phosphate as a substrate. MJ0400 also acts as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, this indicates that MJ0400 is involved in both the carbon metabolism and the shikimate pathway in Methanocaldococcus jannaschii
692386
Methanocaldococcus jannaschii
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.2.1.10
50
-
assay at
Methanocaldococcus jannaschii
4.1.2.13
50
-
assay at, temperature optimum for the specific acitivity of MJ0400-His6
Methanocaldococcus jannaschii
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.2.1.10
25
85
10% activity at 25°C, higher activity at 50°C, optimal activity probably at or above the optimal growth temperature of 85°C
Methanocaldococcus jannaschii
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.2.1.10
80
-
10 mg/ml purified recombinant His6-tagged enzyme, 50 mM Tris-HCl, pH 7.5, 1 mM DTT, half-life is 37 h
Methanocaldococcus jannaschii
2.2.1.10
100
-
10 mg/ml purified recombinant His6-tagged enzyme, 50 mM Tris-HCl, pH 7.5, 1 mM DTT, half-life is 1 h
Methanocaldococcus jannaschii
4.1.2.13
additional information
-
displays heat stability with a half-life of 1 h at 100°C
Methanocaldococcus jannaschii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.2.1.10
7.5
-
assay at
Methanocaldococcus jannaschii
General Information
EC Number
General Information
Commentary
Organism
2.2.1.10
metabolism
the enzyme is involved in both carbon metabolism and amino acid biosynthesis, it catalyzes as transaldolase a step leading to biosynthesis of 3-dehydroquinate, which enters the shikimate pathway, and also shows activity as fructose 1,6-bisphosphate aldolase in carbon metabolism, overview
Methanocaldococcus jannaschii
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.2.1.10
metabolism
the enzyme is involved in both carbon metabolism and amino acid biosynthesis, it catalyzes as transaldolase a step leading to biosynthesis of 3-dehydroquinate, which enters the shikimate pathway, and also shows activity as fructose 1,6-bisphosphate aldolase in carbon metabolism, overview
Methanocaldococcus jannaschii