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Literature summary extracted from

  • Goedl, C.; Nidetzky, B.
    The phosphate site of trehalose phosphorylase from Schizophyllum commune probed by site-directed mutagenesis and chemical rescue studies (2008), FEBS J., 275, 903-913.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.231 guanidine no effect on wild-type but guanidine causes a 45fold enhancement of mutant R507A activity Schizophyllum commune
2.4.1.231 propargylamine no effect on wild-type but propagylamine causes a 23fold enhancement of mutant K512A activity Schizophyllum commune

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.231 K512A kcat/Km (phosphate): 0.0014/Msec, vanadate shows a weaker inhibitory effect compared to wild-type, propargylamine leads to a 23fold increase in activity, phosphate 50 mM enhances basal hydrolase activity Schizophyllum commune
2.4.1.231 R507A Km (phosphate) 103 mM, kcat/Km (phosphate): 0.0004/Msec, vanadate shows no inhibitory effect, guanidine leads to a 45fold increase in activity Schizophyllum commune

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.231 phosphate phosphate 50 mM causes total supression in wild-type but enhances mutant K512 activity Schizophyllum commune
2.4.1.231 vanadate strong inhibitor for wildtype ScTPase and weak inhibitor for mutant K512A but no mutant R507A is not inhibited at all Schizophyllum commune

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.231 additional information
-
 additional information mutant K512A (phosphate): no saturation, Kcat/Km (phosphate): 0.0014/Msec Schizophyllum commune
2.4.1.231 0.8
-
 phosphate wild-type, Kcat/Km (phosphate): 21000/Msec Schizophyllum commune
2.4.1.231 103
-
 phosphate mutant R507A, Kcat/Km (phosphate): 0.0004/Msec Schizophyllum commune

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.231 Schizophyllum commune
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.231 alpha,alpha-trehalose + phosphate
-
 Schizophyllum commune alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.231 ScTPase
-
 Schizophyllum commune
2.4.1.231 trehalose phosphorylase
-
 Schizophyllum commune

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.231 30
-
 assay at Schizophyllum commune

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.231 6.6
-
 assay at Schizophyllum commune

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.231 0.0004
-
 vanadate wild-type Schizophyllum commune
2.4.1.231 0.298
-
 vanadate mutant K512A Schizophyllum commune