Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rushworth, C.A.; Guy, J.L.; Turner, A.J.
    Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identified by site-directed mutagenesis (2008), FEBS J., 275, 6033-6042.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.23 expression in HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.17.23 K481Q angiotensin I cleavage activity is 21% of wild-type activity, angiotensin II cleavage activity is 71.8% of wild-type activity Homo sapiens
3.4.17.23 R169Q angiotensin I cleavage activity is 5.2% of wild-type activity, angiotensin II cleavage activity is 1.1% of wild-type activity. The mutant enzyme does not show any activity with angiotensin I in the absence of chloride ions Homo sapiens
3.4.17.23 R169QK481QR514Q angiotensin I cleavage activity is 53.2% of wild-type activity, angiotensin II cleavage activity is 203.4% of wild-type activity Homo sapiens
3.4.17.23 R514Q angiotensin I cleavage activity is 52% of wild-type activity, angiotensin II cleavage activity is 179.3% of wild-type activity, enhancement of angiotensin II cleavage is a result of a 2.5-fold increase in Vmax compared with the wild-type Homo sapiens
3.4.17.23 W271A angiotensin I cleavage activity is 5.3% of wild-type activity, angiotensin II cleavage activity is 0.9% of wild-type activity. Lacks any significant chloride sensitivity with the substrate angiotensin I Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.23 Cl- ACE2 activity is regulated by chloride ions. The presence of chloride increases the hydrolysis of angiotensin I by ACE2, but inhibits cleavage of the vasoconstrictor angiotensin II Homo sapiens
3.4.17.23 MLN-4760 ACE2-specific inhibitor. Inhibition of wild-type ACE2 was sensitive to chloride concentration Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.23 0.053
-
 angiotensin II pH 7.4, 37°C, mutant enzyme R514Q Homo sapiens
3.4.17.23 0.0586
-
 angiotensin II pH 7.4, 37°C, wild-type enzyme Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.23 Cl- ACE2 activity is regulated by chloride ions. The presence of chloride increases the hydrolysis of angiotensin I by ACE2, but inhibits cleavage of the vasoconstrictor angiotensin II Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.23 Homo sapiens Q9BYF1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.23 angiotensin I + H2O
-
 Homo sapiens angiotensin-(1-9) + Leu
-
 ?
3.4.17.23 angiotensin II + H2O
-
 Homo sapiens angiotensin-(1-7) + Phe
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.17.23 ACE2
-
 Homo sapiens