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Literature summary extracted from

  • Kim, S.K.; Reddy, S.K.; Nelson, B.C.; Robinson, H.; Reddy, P.T.; Ladner, J.E.
    A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H(37)R(v) and the secreted chorismate mutase (y2828) from Yersinia pestis (2008), FEBS J., 275, 4824-4835.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.5 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis
5.4.99.5 expressed in Escherichia coli BL21(DE3) cells Yersinia pestis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.99.5 hanging drop vapour diffusion method, in 2 M ammonium sulfate, 0.1 M citrate/phosphate buffer, pH 4.2 Yersinia pestis
5.4.99.5 sitting drop vapour diffusion method, using 0.1 M Tris-HCl (pH 8.6), 0.2 M MgCl2, and 20% poly(ethylene glycol) 400 for the 90 amino acid enzyme 90-MtCM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.5 0.5
-
 chorismate at 37°C and pH 7.5 Yersinia pestis
5.4.99.5 1.5
-
 chorismate at 37°C and pH 7.5 Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.4.99.5 periplasm
-
 Yersinia pestis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.5 6000
-
 2 * 6000, SDS-PAGE Mycobacterium tuberculosis
5.4.99.5 10090
-
 90-MtCM, MALDI-TOF mass spectrometry and calculated from amino acid sequence Mycobacterium tuberculosis
5.4.99.5 11770
-
 105-MtCM, MALDI-TOF mass spectrometry and calculated from amino acid sequence Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Mycobacterium tuberculosis P9WIC1
-
-
5.4.99.5 Mycobacterium tuberculosis H37Rv P9WIC1
-
-
5.4.99.5 Yersinia pestis Q7CHH5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.5 gel filtration Yersinia pestis
5.4.99.5 subtilisin column chromatography and Sephadex G-75 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate
-
 Mycobacterium tuberculosis Prephenate
-
 ?
5.4.99.5 Chorismate
-
 Yersinia pestis Prephenate
-
 ?
5.4.99.5 Chorismate
-
 Mycobacterium tuberculosis H37Rv Prephenate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.5 homodimer x-ray crystallography Yersinia pestis
5.4.99.5 homodimer 2 * 6000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
5.4.99.5 105-MtCM
-
 Mycobacterium tuberculosis
5.4.99.5 90-MtCM
-
 Mycobacterium tuberculosis
5.4.99.5 Rv0948c
-
 Mycobacterium tuberculosis
5.4.99.5 y2828
-
 Yersinia pestis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.5 5.5
-
 chorismate at 37°C and pH 7.5 Mycobacterium tuberculosis
5.4.99.5 70
-
 chorismate at 37°C and pH 7.5 Yersinia pestis