BRENDA - Enzyme Database show

Characterization of a thermostable dihydrodipicolinate synthase from Thermoanaerobacter tengcongensis

Wolterink-van Loo, S.; Levisson, M.; Cabrieres, M.C.; Franssen, M.C.; van der Oost, J.; Extremophiles 12, 461-469 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.3.3.7
expressed in Escherichia coli BL21(DE3), pET24d vector
Caldanaerobacter subterraneus subsp. tengcongensis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
(S)-lysine
increasing amounts of (S)-lysine (0-200 mM) added, not inhibited under physiological conditions, binding site of the allosteric inhibitor lysine appears not to be conserved
Caldanaerobacter subterraneus subsp. tengcongensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.38
-
(S)-aspartate 4-semialdehyde
by adding 20 mM pyruvate, at 60C
Caldanaerobacter subterraneus subsp. tengcongensis
4.3.3.7
0.85
-
pyruvate
by adding 2 mM (S)-aspartate 4-semialdehyde, at 60C
Caldanaerobacter subterraneus subsp. tengcongensis
4.3.3.7
0.92
-
(S)-aspartate 4-semialdehyde
modeled with substrate inhibition, at 60C
Caldanaerobacter subterraneus subsp. tengcongensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
34000
-
size of monomer inccluding His-tag, calculated from sequence
Caldanaerobacter subterraneus subsp. tengcongensis
4.3.3.7
150000
-
native protein, gel filtration
Caldanaerobacter subterraneus subsp. tengcongensis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
Caldanaerobacter subterraneus subsp. tengcongensis
-
dihydrodipicolinate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Caldanaerobacter subterraneus subsp. tengcongensis
Q8RBI5
DSM 15242T, JCM11007
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
gel filtration
Caldanaerobacter subterraneus subsp. tengcongensis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
specific activity relatively high even at 30C, structural model reveals that the active site is well conserved
Caldanaerobacter subterraneus subsp. tengcongensis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
-
692255
Caldanaerobacter subterraneus subsp. tengcongensis
dihydrodipicolinate + H2O
-
-
-
?
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
characterization of thermostable enzyme features, substrate specificity tested, activity measurements at elevated temperature of 60C
692255
Caldanaerobacter subterraneus subsp. tengcongensis
dihydrodipicolinate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.3.3.7
tetramer
similar to oligomeric states of other homologous enzyme
Caldanaerobacter subterraneus subsp. tengcongensis
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.3.3.7
85
-
specific condensation of pyruvate and (S)-aspartate 4-semialdehyde optimally at, assay performed at 60C, structural model reveals that the active site is well conserved
Caldanaerobacter subterraneus subsp. tengcongensis
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4.3.3.7
30
90
activity analyzed at
Caldanaerobacter subterraneus subsp. tengcongensis
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.3.3.7
90
-
3 h at
Caldanaerobacter subterraneus subsp. tengcongensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.3.7
8
-
specific condensation of pyruvate and (S)-aspartate 4-semialdehyde optimally at
Caldanaerobacter subterraneus subsp. tengcongensis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.3.3.7
4
8
activity analyzed at
Caldanaerobacter subterraneus subsp. tengcongensis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.3.3.7
4.7
-
(S)-lysine
180 mM, at 60C
Caldanaerobacter subterraneus subsp. tengcongensis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
expressed in Escherichia coli BL21(DE3), pET24d vector
Caldanaerobacter subterraneus subsp. tengcongensis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
(S)-lysine
increasing amounts of (S)-lysine (0-200 mM) added, not inhibited under physiological conditions, binding site of the allosteric inhibitor lysine appears not to be conserved
Caldanaerobacter subterraneus subsp. tengcongensis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.3.3.7
4.7
-
(S)-lysine
180 mM, at 60C
Caldanaerobacter subterraneus subsp. tengcongensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.38
-
(S)-aspartate 4-semialdehyde
by adding 20 mM pyruvate, at 60C
Caldanaerobacter subterraneus subsp. tengcongensis
4.3.3.7
0.85
-
pyruvate
by adding 2 mM (S)-aspartate 4-semialdehyde, at 60C
Caldanaerobacter subterraneus subsp. tengcongensis
4.3.3.7
0.92
-
(S)-aspartate 4-semialdehyde
modeled with substrate inhibition, at 60C
Caldanaerobacter subterraneus subsp. tengcongensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
34000
-
size of monomer inccluding His-tag, calculated from sequence
Caldanaerobacter subterraneus subsp. tengcongensis
4.3.3.7
150000
-
native protein, gel filtration
Caldanaerobacter subterraneus subsp. tengcongensis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
Caldanaerobacter subterraneus subsp. tengcongensis
-
dihydrodipicolinate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
gel filtration
Caldanaerobacter subterraneus subsp. tengcongensis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
specific activity relatively high even at 30C, structural model reveals that the active site is well conserved
Caldanaerobacter subterraneus subsp. tengcongensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
-
692255
Caldanaerobacter subterraneus subsp. tengcongensis
dihydrodipicolinate + H2O
-
-
-
?
4.3.3.7
(S)-aspartate 4-semialdehyde + pyruvate
characterization of thermostable enzyme features, substrate specificity tested, activity measurements at elevated temperature of 60C
692255
Caldanaerobacter subterraneus subsp. tengcongensis
dihydrodipicolinate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.3.7
tetramer
similar to oligomeric states of other homologous enzyme
Caldanaerobacter subterraneus subsp. tengcongensis
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.3.3.7
85
-
specific condensation of pyruvate and (S)-aspartate 4-semialdehyde optimally at, assay performed at 60C, structural model reveals that the active site is well conserved
Caldanaerobacter subterraneus subsp. tengcongensis
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4.3.3.7
30
90
activity analyzed at
Caldanaerobacter subterraneus subsp. tengcongensis
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.3.3.7
90
-
3 h at
Caldanaerobacter subterraneus subsp. tengcongensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.3.7
8
-
specific condensation of pyruvate and (S)-aspartate 4-semialdehyde optimally at
Caldanaerobacter subterraneus subsp. tengcongensis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.3.3.7
4
8
activity analyzed at
Caldanaerobacter subterraneus subsp. tengcongensis