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Literature summary extracted from

  • Fu, J.; Huang, H.; Meng, K.; Yuan, T.; Yao, B.; Shi, Y.; Ouyang, P.
    A novel cold-adapted phospholipase A1 from Serratia sp. xjF1: Gene cloning, expression and characterization (2008), Enzyme Microb. Technol., 42, 187-194.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.32 expressed in Pichia pastoris Serratia sp. xJF1

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.32 Cr3+ 51.4% relative activity at 5 mM Serratia sp. xJF1
3.1.1.32 Cu2+ 56.8% relative activity at 5 mM Serratia sp. xJF1
3.1.1.32 EDTA complete inhibition at 5 mM Serratia sp. xJF1
3.1.1.32 Fe2+ 89.2% relative activity at 5 mM Serratia sp. xJF1
3.1.1.32 Mg2+ 13.5% relative activity at 5 mM Serratia sp. xJF1
3.1.1.32 Mn2+ complete inhibition at 5 mM Serratia sp. xJF1
3.1.1.32 Na+ 54.7% relative activity at 5 mM Serratia sp. xJF1
3.1.1.32 SDS 20.9% relative activity at 5 mM Serratia sp. xJF1
3.1.1.32 Triton X-100 complete inhibition at 5 mM Serratia sp. xJF1
3.1.1.32 Zn2+ 52% relative activity at 5 mM Serratia sp. xJF1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.32 Ca2+ 111.5% relative activity at 5 mM Ca2+ Serratia sp. xJF1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.32 33800
-
 calculated from amino acid sequence Serratia sp. xJF1
3.1.1.32 35000
-
 SDS-PAGE Serratia sp. xJF1

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Serratia sp. xJF1 A9X3M0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.32 Q sepharose column chromatography Serratia sp. xJF1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.32 202.3
-
 recombinant enzyme, after 8.5fold purification, at pH 9.0 and 35°C Serratia sp. xJF1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 phosphatidylcholine + H2O
-
 Serratia sp. xJF1 2-acylglycerophosphocholine + a fatty acid anion
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.32 PLA1
-
 Serratia sp. xJF1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.32 35
-
 recombinant enzyme Serratia sp. xJF1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.32 10 55 the enzyme loses most of its activity at 55°C but retains about 70% of its maximum activity at 10°C, heat treatment at 60°C for 10 min results in an 86% decrease in enzyme activity Serratia sp. xJF1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.32 9
-
-
 Serratia sp. xJF1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.32 5.5 10 the enzyme is stable between pH 6 and 10 at the indicated pH range when incubated at 35°C for 3 h, but the enzyme loses about 50% of its activity at pHs below 5.5 Serratia sp. xJF1