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Literature summary extracted from

  • Ahn, S.J.; Kim, N.Y.; Jeon, S.J.; Sung, J.H.; Je, J.E.; Seo, J.S.; Kim, M.S.; Kim, J.K.; Chung, J.K.; Lee, H.H.
    Molecular cloning, tissue distribution and enzymatic characterization of cathepsin X from olive flounder (Paralichthys olivaceus) (2008), Comp. Biochem. Physiol. B, 151, 203-212.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.18.1 cDNA encoding for the proenzyme of PoCtX (proPoCtX) is expressed in Escherichia coli as a 57 kDa fusion protein with glutathione S-transferase Paralichthys olivaceus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.18.1 1,10-phenanthroline 0.1 mM, proPoCtX: 51.3% inhibition, native PoCtX: 56.6% inhibition Paralichthys olivaceus
3.4.18.1 antipain 0.1 mM, proPoCtX: 98.6% inhibition, native PoCtX: 97.6% inhibition Paralichthys olivaceus
3.4.18.1 Aprotinin 0.1 mM, proPoCtX: 31.9% inhibition, native PoCtX: 51.3% inhibition Paralichthys olivaceus
3.4.18.1 chymostatin 0.1 mM, proPoCtX: 35.4% inhibition, native PoCtX: 96.9% inhibition Paralichthys olivaceus
3.4.18.1 E-64 0.1 mM, proPoCtX: 25.6% inhibition, native PoCtX: 98.6% inhibition Paralichthys olivaceus
3.4.18.1 EDTA 0.1 mM, proPoCtX: 5.1% inhibition, native PoCtX: 14.2% inhibition Paralichthys olivaceus
3.4.18.1 EGTA 0.1 mM, proPoCtX: 2.2% inhibition, native PoCtX: 25.6% inhibition Paralichthys olivaceus
3.4.18.1 leupeptin 0.1 mM, proPoCtX: 99.1% inhibition, native PoCtX: 95.6% inhibition Paralichthys olivaceus
3.4.18.1 additional information recombinant proPoCtX is inhibited by antipain and leupeptin Paralichthys olivaceus
3.4.18.1 NEM 0.1 mM, proPoCtX: 44.6% inhibition, native PoCtX: 67.0% inhibition Paralichthys olivaceus
3.4.18.1 pepstatin A 0.1 mM, proPoCtX: 0% inhibition, native PoCtX: 36.0% inhibition Paralichthys olivaceus
3.4.18.1 PMSF 0.1 mM, proPoCtX: 42.2% inhibition, native PoCtX: 37.2% inhibition Paralichthys olivaceus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.18.1 cytosol
-
 Paralichthys olivaceus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.18.1 33200
-
 native PoCtX protein Paralichthys olivaceus

Organism

EC Number Organism UniProt Comment Textmining
3.4.18.1 Paralichthys olivaceus Q58HF4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.18.1 purified 9.48fold via a one-step purification process using a DEAE-Sephagel HPLC column Paralichthys olivaceus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.18.1 alimentary canal
-
 Paralichthys olivaceus
-
3.4.18.1 brain
-
 Paralichthys olivaceus
-
3.4.18.1 eye
-
 Paralichthys olivaceus
-
3.4.18.1 gill
-
 Paralichthys olivaceus
-
3.4.18.1 heart
-
 Paralichthys olivaceus
-
3.4.18.1 intestine
-
 Paralichthys olivaceus
-
3.4.18.1 kidney
-
 Paralichthys olivaceus
-
3.4.18.1 liver
-
 Paralichthys olivaceus
-
3.4.18.1 muscle
-
 Paralichthys olivaceus
-
3.4.18.1 pyloric cecum
-
 Paralichthys olivaceus
-
3.4.18.1 spleen
-
 Paralichthys olivaceus
-
3.4.18.1 stomach
-
 Paralichthys olivaceus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.18.1 364.5
-
 cytosol extract Paralichthys olivaceus
3.4.18.1 3457
-
 DEAE-Sephagel, 9.48fold purification Paralichthys olivaceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.18.1 Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Paralichthys olivaceus ?
-
 ?
3.4.18.1 Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Paralichthys olivaceus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.18.1 cathepsin X
-
 Paralichthys olivaceus
3.4.18.1 PoCtX
-
 Paralichthys olivaceus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.18.1 37
-
 assay at Paralichthys olivaceus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.18.1 5
-
 recombinant proPoCtX Paralichthys olivaceus
3.4.18.1 6
-
 native PoCtX protein Paralichthys olivaceus