BRENDA - Enzyme Database show

Structural, functional and calorimetric investigation of MosA, a dihydrodipicolinate synthase from Sinorhizobium meliloti l5-30, does not support involvement in rhizopine biosynthesis

Phenix, C.P.; Nienaber, K.; Tam, P.H.; Delbaere, L.T.; Palmer, D.R.; ChemBioChem 9, 1591-1602 (2008)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
4.3.3.7
structure of MosA protein solved to 1.95 A resolution, data collection and refinement statistics
Sinorhizobium meliloti
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
2-oxobutyrate
forms a Schiff base with MosA protein, competitive inhibition
Sinorhizobium meliloti
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
Sinorhizobium meliloti
-
dihydrodipicolinate + H2O
-
-
?
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
Sinorhizobium meliloti L5-30
-
dihydrodipicolinate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Sinorhizobium meliloti
Q07607
L5-30
-
4.3.3.7
Sinorhizobium meliloti L5-30
Q07607
L5-30
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
analysis of ability of rhizopines to interact with MosA protein in the presence and absence of methyl donors, no methyltransferase activity observed in the presence of scyllo-inosamine and S-adenosylmethionine (SAM), presence of rhizopine compounds does not affect kinetics of dihydrodipicolinate synthesis
Sinorhizobium meliloti
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
-
691786
Sinorhizobium meliloti
dihydrodipicolinate + H2O
-
-
-
?
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
isothermal titration calorimetry (ITC) to study Schiff base formation between an enzyme and its substrate, pyruvate condenses with an active site lysine residue (Lys161 in MosA)
691786
Sinorhizobium meliloti
dihydrodipicolinate + H2O
-
-
-
?
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
-
691786
Sinorhizobium meliloti L5-30
dihydrodipicolinate + H2O
-
-
-
?
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
isothermal titration calorimetry (ITC) to study Schiff base formation between an enzyme and its substrate, pyruvate condenses with an active site lysine residue (Lys161 in MosA)
691786
Sinorhizobium meliloti L5-30
dihydrodipicolinate + H2O
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.3.7
25
-
isothermal titration microcalorimetry at
Sinorhizobium meliloti
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.3.3.7
structure of MosA protein solved to 1.95 A resolution, data collection and refinement statistics
Sinorhizobium meliloti
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
2-oxobutyrate
forms a Schiff base with MosA protein, competitive inhibition
Sinorhizobium meliloti
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
Sinorhizobium meliloti
-
dihydrodipicolinate + H2O
-
-
?
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
Sinorhizobium meliloti L5-30
-
dihydrodipicolinate + H2O
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
analysis of ability of rhizopines to interact with MosA protein in the presence and absence of methyl donors, no methyltransferase activity observed in the presence of scyllo-inosamine and S-adenosylmethionine (SAM), presence of rhizopine compounds does not affect kinetics of dihydrodipicolinate synthesis
Sinorhizobium meliloti
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
-
691786
Sinorhizobium meliloti
dihydrodipicolinate + H2O
-
-
-
?
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
isothermal titration calorimetry (ITC) to study Schiff base formation between an enzyme and its substrate, pyruvate condenses with an active site lysine residue (Lys161 in MosA)
691786
Sinorhizobium meliloti
dihydrodipicolinate + H2O
-
-
-
?
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
-
691786
Sinorhizobium meliloti L5-30
dihydrodipicolinate + H2O
-
-
-
?
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
isothermal titration calorimetry (ITC) to study Schiff base formation between an enzyme and its substrate, pyruvate condenses with an active site lysine residue (Lys161 in MosA)
691786
Sinorhizobium meliloti L5-30
dihydrodipicolinate + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.3.7
25
-
isothermal titration microcalorimetry at
Sinorhizobium meliloti