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Literature summary extracted from

  • Phenix, C.P.; Nienaber, K.; Tam, P.H.; Delbaere, L.T.; Palmer, D.R.
    Structural, functional and calorimetric investigation of MosA, a dihydrodipicolinate synthase from Sinorhizobium meliloti l5-30, does not support involvement in rhizopine biosynthesis (2008), ChemBioChem, 9, 1591-1602.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.7 structure of MosA protein solved to 1.95 A resolution, data collection and refinement statistics Sinorhizobium meliloti

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.7 2-oxobutyrate forms a Schiff base with MosA protein, competitive inhibition Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.7 L-aspartate 4-semialdehyde + pyruvate Sinorhizobium meliloti
-
 dihydrodipicolinate + H2O
-
 ?
4.3.3.7 L-aspartate 4-semialdehyde + pyruvate Sinorhizobium meliloti L5-30
-
 dihydrodipicolinate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.7 Sinorhizobium meliloti Q07607 L5-30
-
4.3.3.7 Sinorhizobium meliloti L5-30 Q07607 L5-30
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.3.7 additional information
-
 analysis of ability of rhizopines to interact with MosA protein in the presence and absence of methyl donors, no methyltransferase activity observed in the presence of scyllo-inosamine and S-adenosylmethionine (SAM), presence of rhizopine compounds does not affect kinetics of dihydrodipicolinate synthesis Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.7 L-aspartate 4-semialdehyde + pyruvate
-
 Sinorhizobium meliloti dihydrodipicolinate + H2O
-
 ?
4.3.3.7 L-aspartate 4-semialdehyde + pyruvate isothermal titration calorimetry (ITC) to study Schiff base formation between an enzyme and its substrate, pyruvate condenses with an active site lysine residue (Lys161 in MosA) Sinorhizobium meliloti dihydrodipicolinate + H2O
-
 ?
4.3.3.7 L-aspartate 4-semialdehyde + pyruvate
-
 Sinorhizobium meliloti L5-30 dihydrodipicolinate + H2O
-
 ?
4.3.3.7 L-aspartate 4-semialdehyde + pyruvate isothermal titration calorimetry (ITC) to study Schiff base formation between an enzyme and its substrate, pyruvate condenses with an active site lysine residue (Lys161 in MosA) Sinorhizobium meliloti L5-30 dihydrodipicolinate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.3.7 DHDPS
-
 Sinorhizobium meliloti
4.3.3.7 MosA protein
-
 Sinorhizobium meliloti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.3.7 25
-
 isothermal titration microcalorimetry at Sinorhizobium meliloti