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Literature summary extracted from

  • Troccaz, M.; Benattia, F.; Borchard, G.; Clark, A.J.
    Properties of recombinant Staphylococcus haemolyticus cystathionine beta-lyase (metC) and its potential role in the generation of volatile thiols in axillary malodor (2008), Chem. Biodivers., 5, 2372-2385.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.13 gene metC or SH2636, phylogenetic tree and analysis, cloning from strains AX3 and CSC1435, expression of His-tagged enzyme in Escherichia coli strain BL21 Staphylococcus haemolyticus

General Stability

EC Number General Stability Organism
4.4.1.13 addition of 15% glycerol extends the enzymatic lifetime to several days and permitted freezing of the preparation at -20°C Staphylococcus haemolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.13 ammonium sulfate 66% inhibition at 4 mM Staphylococcus haemolyticus
4.4.1.13 glycine 73% inhibition at 4 mM Staphylococcus haemolyticus
4.4.1.13 L-cystathionine substrate inhibition at concentrations above 6 mM Staphylococcus haemolyticus
4.4.1.13 L-methionine 40% inhibition at 6 mM Staphylococcus haemolyticus
4.4.1.13 L-serine 86% inhibition at 4 mM Staphylococcus haemolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.13 additional information
-
additional information kinetics, overview Staphylococcus haemolyticus
4.4.1.13 1.2
-
L-cystathionine pH 8.0, 37°C, purified recombinant enzyme Staphylococcus haemolyticus
4.4.1.13 1.3
-
L-cystathionine pH 8.5, 37°C, purified recombinant enzyme Staphylococcus haemolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.13 42700
-
4 * 46000, recombinant His-tagged enzyme, SDS-PAGE, 4 * 42700, detagged enzyme, SDS-PAGE Staphylococcus haemolyticus
4.4.1.13 46000
-
4 * 46000, recombinant His-tagged enzyme, SDS-PAGE, 4 * 42700, detagged enzyme, SDS-PAGE Staphylococcus haemolyticus
4.4.1.13 170000
-
recombinant enzyme, gel filtration Staphylococcus haemolyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.13 L-cystathionine + H2O Staphylococcus haemolyticus penultimate step in microbial and plant methionine biosynthesis L-homocysteine + NH3 + pyruvate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Staphylococcus haemolyticus
-
strains AX3 and JCSC1435, gene metC or SH2636
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13 recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Staphylococcus haemolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 L-cystathionine + H2O penultimate step in microbial and plant methionine biosynthesis Staphylococcus haemolyticus L-homocysteine + NH3 + pyruvate
-
?
4.4.1.13 L-cystathionine + H2O the enzyme catalyzes the split of the a C-N and of the beta C-S bonds through a beta-elimination Staphylococcus haemolyticus L-homocysteine + NH3 + pyruvate
-
?
4.4.1.13 L-cysteine + H2O very low activity Staphylococcus haemolyticus sulfide + NH3 + pyruvate
-
?
4.4.1.13 L-methionine + H2O
-
Staphylococcus haemolyticus methanethiol + NH3 + 2-oxobutanoate
-
?
4.4.1.13 additional information no activity with cysteine, cysteinyl, or cysteinylglycine-S-precursors (S-[1-(2-hydroxymethyl)-1-methylbutyl]-lcysteinylglycine and S-[1-(2-hydroxymethyl)-1-methylbutyl]-l-cysteine) Staphylococcus haemolyticus ?
-
?
4.4.1.13 S-[1-(2-hydroxyethyl)-1-methylbutyl]-L-cysteine + H2O very low activity Staphylococcus haemolyticus sulfanylhexanol + ?
-
?
4.4.1.13 S-[1-(2-hydroxyethyl)-1-methylbutyl]-L-cysteinylglycine + H2O very low activity Staphylococcus haemolyticus sulfanylhexanol + ?
-
?

Subunits

EC Number Subunits Comment Organism
4.4.1.13 tetramer 4 * 46000, recombinant His-tagged enzyme, SDS-PAGE, 4 * 42700, detagged enzyme, SDS-PAGE Staphylococcus haemolyticus

Synonyms

EC Number Synonyms Comment Organism
4.4.1.13 beta-cystathionase
-
Staphylococcus haemolyticus
4.4.1.13 CBL
-
Staphylococcus haemolyticus
4.4.1.13 More the enzyme belongs to the large family of cysteine-S-conjugate beta-lyases Staphylococcus haemolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.13 37
-
-
Staphylococcus haemolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.13 5.5
-
reaction with methionine Staphylococcus haemolyticus
4.4.1.13 8.5
-
reaction with L-cystathionine Staphylococcus haemolyticus

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.13 pyridoxal 5'-phosphate dependent on Staphylococcus haemolyticus