BRENDA - Enzyme Database show

Expression and purification of human mPGES-1 in E. coli and identification of inhibitory compounds from a drug-library

Kim, W.I.; Choi, K.A.; Do, H.S.; Yu, Y.G.; BMB Rep. 41, 808-813 (2008)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
5.3.99.3
medicine
the selective inhibition of mPGES-1 generates anti-inflammatory effects without unwanted side effects involving homeostasis
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.3
into the TA vector and subsequently into pET-28a for expression in various Escherichia coli strains, the expression rate is enhanced by modifying the utility of specific codons
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.3
dyphylline
-
Homo sapiens
5.3.99.3
Oxacillin
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.0024
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
membrane
-
Homo sapiens
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.3
18800
-
determined by SDS-PAGE and Western Blot analysis
Homo sapiens
5.3.99.3
19000
-
calculated, His-tagged mPGES-1
Homo sapiens
5.3.99.3
57000
-
trimer, determined by SDS-PAGE and Western Blot analysis
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.3
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
-
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.3
using a Ni-NTA and a Mono Q-Sepharose column
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
691476
Homo sapiens
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.3
trimer
trimeric state characterized by chemical cross-linking with glutaraldehyde
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.3.99.3
20
-
activity assay at room temperature
Homo sapiens
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
5.3.99.3
0.11
-
-
Homo sapiens
Oxacillin
5.3.99.3
0.23
-
-
Homo sapiens
dyphylline
Application (protein specific)
EC Number
Application
Commentary
Organism
5.3.99.3
medicine
the selective inhibition of mPGES-1 generates anti-inflammatory effects without unwanted side effects involving homeostasis
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
into the TA vector and subsequently into pET-28a for expression in various Escherichia coli strains, the expression rate is enhanced by modifying the utility of specific codons
Homo sapiens
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
5.3.99.3
0.11
-
-
Homo sapiens
Oxacillin
5.3.99.3
0.23
-
-
Homo sapiens
dyphylline
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.3
dyphylline
-
Homo sapiens
5.3.99.3
Oxacillin
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.0024
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
membrane
-
Homo sapiens
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.3
18800
-
determined by SDS-PAGE and Western Blot analysis
Homo sapiens
5.3.99.3
19000
-
calculated, His-tagged mPGES-1
Homo sapiens
5.3.99.3
57000
-
trimer, determined by SDS-PAGE and Western Blot analysis
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.3
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
-
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
using a Ni-NTA and a Mono Q-Sepharose column
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
691476
Homo sapiens
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.3
trimer
trimeric state characterized by chemical cross-linking with glutaraldehyde
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.3.99.3
20
-
activity assay at room temperature
Homo sapiens