BRENDA - Enzyme Database

A novel extracellular phospholipase C purified from a marine bacterium, Pseudoalteromonas sp. J937

Mo, S.; Kim, J.H.; Cho, K.W.; Biotechnol. Lett. 31, 89-94 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
Ca2+
activity is enhanced by 150% with Ca2+ (200 mM)
Pseudoalteromonas sp.
3.1.4.3
Na+
activity is enhanced by 180% with Na+ (500 mM)
Pseudoalteromonas sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Pseudoalteromonas sp.
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
32000
-
SDS-PAGE
Pseudoalteromonas sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Pseudoalteromonas sp.
-
J937
-
3.1.4.3
Pseudoalteromonas sp. J937
-
J937
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.3
by serial ion exchange and gel filtration column chromatography
Pseudoalteromonas sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.3
additional information
-
specific activity of 33 U/mg protein
Pseudoalteromonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
additional information
phospholipase C hydrolyzes phosphatidylethanolamine and phosphatidylcholine but not other glycerophospholipids
691450
Pseudoalteromonas sp.
?
-
-
-
-
3.1.4.3
additional information
phospholipase C hydrolyzes phosphatidylethanolamine and phosphatidylcholine but not other glycerophospholipids
691450
Pseudoalteromonas sp. J937
?
-
-
-
-
3.1.4.3
phosphatidylcholine + H2O
-
691450
Pseudoalteromonas sp.
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
3.1.4.3
phosphatidylcholine + H2O
-
691450
Pseudoalteromonas sp. J937
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
3.1.4.3
phosphatidylethanolamine + H2O
-
691450
Pseudoalteromonas sp.
phosphorylethanolamine + 1,2-diacylglycerol
-
-
-
?
3.1.4.3
phosphatidylethanolamine + H2O
-
691450
Pseudoalteromonas sp. J937
phosphorylethanolamine + 1,2-diacylglycerol
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.3
45
-
-
Pseudoalteromonas sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
8
-
-
Pseudoalteromonas sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
Ca2+
activity is enhanced by 150% with Ca2+ (200 mM)
Pseudoalteromonas sp.
3.1.4.3
Na+
activity is enhanced by 180% with Na+ (500 mM)
Pseudoalteromonas sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Pseudoalteromonas sp.
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
32000
-
SDS-PAGE
Pseudoalteromonas sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.3
by serial ion exchange and gel filtration column chromatography
Pseudoalteromonas sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.3
additional information
-
specific activity of 33 U/mg protein
Pseudoalteromonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
additional information
phospholipase C hydrolyzes phosphatidylethanolamine and phosphatidylcholine but not other glycerophospholipids
691450
Pseudoalteromonas sp.
?
-
-
-
-
3.1.4.3
additional information
phospholipase C hydrolyzes phosphatidylethanolamine and phosphatidylcholine but not other glycerophospholipids
691450
Pseudoalteromonas sp. J937
?
-
-
-
-
3.1.4.3
phosphatidylcholine + H2O
-
691450
Pseudoalteromonas sp.
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
3.1.4.3
phosphatidylcholine + H2O
-
691450
Pseudoalteromonas sp. J937
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
3.1.4.3
phosphatidylethanolamine + H2O
-
691450
Pseudoalteromonas sp.
phosphorylethanolamine + 1,2-diacylglycerol
-
-
-
?
3.1.4.3
phosphatidylethanolamine + H2O
-
691450
Pseudoalteromonas sp. J937
phosphorylethanolamine + 1,2-diacylglycerol
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.3
45
-
-
Pseudoalteromonas sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
8
-
-
Pseudoalteromonas sp.