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Literature summary extracted from

  • Sun, S.Y.; Xu, Y.
    Membrane-bound synthetic lipase specifically cultured under solid-state fermentation and submerged fermentation by Rhizopus chinensis: A comparative investigation (2008), Bioresour. Technol., 100, 1336-1342.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 Al3+ SML shows 16% increased activity in the presence of 10 mM Al3+ Rhizopus microsporus var. chinensis
3.1.1.3 K+ SSL shows 22% increased activity in the presence of 10 mM K+ Rhizopus microsporus var. chinensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Al3+ SSL shows 67% residual activity in the presence of 10 mM Al3+ Rhizopus microsporus var. chinensis
3.1.1.3 Ca2+ SSL and SML show 80% and 60% residual activity, respectively, in the presence of 10 mM Ca2+ Rhizopus microsporus var. chinensis
3.1.1.3 Cu2+ SSL and SML show 5% and 30% residual activity, respectively, in the presence of 10 mM Cu2+ Rhizopus microsporus var. chinensis
3.1.1.3 Fe2+ SSL and SML show 73% and 18% residual activity, respectively, in the presence of 10 mM Fe2+ Rhizopus microsporus var. chinensis
3.1.1.3 Fe3+ SSL and SML show 28% and 9% residual activity, respectively, in the presence of 10 mM Fe3+ Rhizopus microsporus var. chinensis
3.1.1.3 Hg2+ SSL and SML show 7% and 9% residual activity, respectively, in the presence of 10 mM Hg2+ Rhizopus microsporus var. chinensis
3.1.1.3 K+ SML shows 85% residual activity in the presence of 10 mM Ki+ Rhizopus microsporus var. chinensis
3.1.1.3 Li+ SSL and SML show 80% and 60% residual activity, respectively, in the presence of 10 mM Li+ Rhizopus microsporus var. chinensis
3.1.1.3 Mg2+ SSL and SML show 54% and 71% residual activity, respectively, in the presence of 10 mM Mg2+ Rhizopus microsporus var. chinensis
3.1.1.3 Mn2+ SSL and SML show 80% and 60% residual activity, respectively, in the presence of 10 mM Mn2+ Rhizopus microsporus var. chinensis
3.1.1.3 additional information not affected by 2-mercaptoethanol and EDTA Rhizopus microsporus var. chinensis
3.1.1.3 phenylmethylsulfonyl fluoride SML shows 32% residual activity in the presence of 10 mM phenylmethylsulfonyl fluoride Rhizopus microsporus var. chinensis
3.1.1.3 SDS SDS shows strong inhibitory effect on SSL and SML, leaving residual activity of 43% and 28%, respectively Rhizopus microsporus var. chinensis
3.1.1.3 Zn2+ SSL and SML show 76% and 55% residual activity, respectively, in the presence of 10 mM Zn2+ Rhizopus microsporus var. chinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 41000
-
SML, calculated from amino acid sequence Rhizopus microsporus var. chinensis
3.1.1.3 63000
-
SSL, calculated from amino acid sequence Rhizopus microsporus var. chinensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Rhizopus microsporus var. chinensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 ammonium sulfate precipitation, butyl Sepharose column chromatography, and Superdex 75 gel filtration Rhizopus microsporus var. chinensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 4.8
-
crude SSL, at 37°C Rhizopus microsporus var. chinensis
3.1.1.3 11.5
-
crude SML, at 37°C Rhizopus microsporus var. chinensis
3.1.1.3 48.8
-
SML after 4.2fold purification, at 37°C Rhizopus microsporus var. chinensis
3.1.1.3 305
-
SSL after 63.5fold purification, at 37°C Rhizopus microsporus var. chinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl acetate + H2O
-
Rhizopus microsporus var. chinensis 4-nitrophenol + acetate
-
?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
Rhizopus microsporus var. chinensis 4-nitrophenol + butyrate
-
?
3.1.1.3 4-nitrophenyl caproate + H2O
-
Rhizopus microsporus var. chinensis 4-nitrophenol + caproate
-
?
3.1.1.3 4-nitrophenyl caprylate + H2O
-
Rhizopus microsporus var. chinensis 4-nitrophenol + caprylate
-
?
3.1.1.3 4-nitrophenyl laurate + H2O
-
Rhizopus microsporus var. chinensis 4-nitrophenol + laurate
-
?
3.1.1.3 4-nitrophenyl palmitate + H2O preferred substrate Rhizopus microsporus var. chinensis 4-nitrophenol + palmitate
-
?
3.1.1.3 4-nitrophenyl propionate + H2O
-
Rhizopus microsporus var. chinensis 4-nitrophenol + propionate
-
?
3.1.1.3 4-nitrophenyl stearate + H2O
-
Rhizopus microsporus var. chinensis 4-nitrophenol + stearate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 monomer
-
Rhizopus microsporus var. chinensis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 SML lipase obtained from submerged fermentation cell culture Rhizopus microsporus var. chinensis
3.1.1.3 SSL lipase obtained from solid-state fermentation cell culture Rhizopus microsporus var. chinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
-
Rhizopus microsporus var. chinensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 20 60
-
Rhizopus microsporus var. chinensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 30 70 SSL shows 57% and 65.2% of its maximum activity at 30°C and 50°C, SML displays approximately 75.9% of its maximal activity at 30°C, SSL retains greater than 80% of the initial activity after 1 h incubation from 30 to 50°C, but the activity is reduced to 44.3% after exposure to 60°C for 1 h, SML is only stable below 40°C, the hydrolytic activity of purified SML decreases dramatically when the temperature increased above 50°C, and 49.7% and 84.1% of the activity is lost at 50 and 60°C after 1 h exposure, SML almost loses all of the original activity at 70°C after 1 h incubation Rhizopus microsporus var. chinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
-
Rhizopus microsporus var. chinensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 8 10
-
Rhizopus microsporus var. chinensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 6 9 more than 80% of the initial activity is conserved at these pHs when incubated at 30°C for 1 h, over 70% of hydrolytic activity is retained at pH 7.0-8.0 for purified SML after 1 h of incubation, the enzyme stability decreases sharply at pH 9.0 and loses 61.7% activity at such pH value, SSL and SML are strongly inhibited by extreme acidic pH (under 5) Rhizopus microsporus var. chinensis