Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Siddiqua, A.; Rehmat, M.; Saeed, A.; Amin, S.; Naz, R.; Sherazi, M.; Khan, G.M.; Saeed, A.
    Acid phosphatases from the liver of Labeo rohita: purification and characterization (2008), Biol. Pharm. Bull., 31, 802-808.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 6-ethylmercaptopurine 1 mM, activity: 281%, isoenzyme 2 Labeo rohita
3.1.3.2 6-ethylmercaptopurine activity: 108% Labeo rohita
3.1.3.2 adenine 1 mM, activity: 194%, isoenzyme 2 Labeo rohita
3.1.3.2 adenosine 1 mM, activity: 134%, isoenzyme 2 Labeo rohita
3.1.3.2 ADP 1 mM, activity: 118%, isoenzyme 2 Labeo rohita
3.1.3.2 AMP 1 mM, activity: 104%, isoenzyme 1 Labeo rohita
3.1.3.2 AMP 1 mM, activity: 162%, isoenzyme 2 Labeo rohita
3.1.3.2 cAMP 1 mM, activity: 106%, isoenzyme 1 Labeo rohita
3.1.3.2 cAMP 1 mM, activity: 126%, isoenzyme 2 Labeo rohita
3.1.3.2 cGMP 1 mM, activity: 106%, isoenzyme 1 Labeo rohita
3.1.3.2 cGMP 1 mM, activity: 250%, isoenzyme 2 Labeo rohita
3.1.3.2 GMP 1 mM, activity: 104%, isoenzyme 1 Labeo rohita
3.1.3.2 GMP 1 mM, activity: 168%, isoenzyme 2 Labeo rohita
3.1.3.2 guanine 1 mM, activity: 107%, isoenzyme 2 Labeo rohita
3.1.3.2 guanosine 1 mM, activity: 299%, isoenzyme 2 Labeo rohita

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 inorganic phosphate
-
 Labeo rohita
3.1.3.2 molybdate
-
 Labeo rohita
3.1.3.2 pyridoxal 5'-phosphate
-
 Labeo rohita
3.1.3.2 pyridoxamine 5'-phosphate
-
 Labeo rohita
3.1.3.2 Tartrate
-
 Labeo rohita
3.1.3.2 vanadate
-
 Labeo rohita

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.11
-
 4-nitrophenyl phosphate pH 5.5, 37°C, isoenzyme 1, Vmax (mmol/min/mg): 0.0093 Labeo rohita
3.1.3.2 0.16
-
 Phenyl phosphate pH 5.5, 37°C, isoenzyme 1, Vmax (mmol/min/mg): 0.0076 Labeo rohita
3.1.3.2 0.18
-
 phosphotyrosine pH 5.5, 37°C, isoenzyme 1, Vmax (mmol/min/mg): 0.0058 Labeo rohita
3.1.3.2 0.2
-
 flavin mononucleotide pH 5.5, 37°C, isoenzyme 1, Vmax (mmol/min/mg): 0.0082 Labeo rohita
3.1.3.2 0.23
-
 4-nitrophenyl phosphate pH 5.5, 37°C, isoenzyme 2, Vmax (mmol/min/mg): 0.038 Labeo rohita
3.1.3.2 0.31
-
 Phenyl phosphate pH 5.5, 37°C, isoenzyme 2, Vmax (mmol/min/mg): 0.032 Labeo rohita
3.1.3.2 0.41
-
 flavin mononucleotide pH 5.5, 37°C, isoenzyme 2, Vmax (mmol/min/mg): 0.0375 Labeo rohita
3.1.3.2 1.1
-
 phosphotyrosine pH 5.5, 37°C, isoenzyme 2, Vmax (mmol/min/mg): 0.0154 Labeo rohita

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 18000
-
 isoenzyme 1, gel filtration Labeo rohita
3.1.3.2 18000
-
 isoenzyme 2, gel filtration Labeo rohita
3.1.3.2 100000
-
 gel filtration Labeo rohita

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Labeo rohita
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 using gel filtration and affinity chromatography. Purification factor: 12.5 Labeo rohita
3.1.3.2 using gel filtration and affinity chromatography. Purification factor: 165 Labeo rohita
3.1.3.2 using gel filtration and affinity chromatography. Purification factor: 900 Labeo rohita

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 liver
-
 Labeo rohita
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 0.5
-
-
 Labeo rohita
3.1.3.2 6.58
-
 isoenzyme 1 Labeo rohita
3.1.3.2 35.6
-
 isoenzyme 2 Labeo rohita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O relative hydrolytic rate: 100% Labeo rohita 4-nitrophenol + phosphate
-
 ?
3.1.3.2 adenosine monophosphate + H2O relative hydrolytic rate: 50% Labeo rohita adenosine + phosphate
-
 ?
3.1.3.2 adenosine triphosphate + H2O relative hydrolytic rate: 25% Labeo rohita ATP + phosphate
-
 ?
3.1.3.2 alpha-glycerophosphate + H2O relative hydrolytic rate: 13% Labeo rohita glycerol + phosphate
-
 ?
3.1.3.2 alpha-glycerophosphate + H2O relative hydrolytic rate: 15% Labeo rohita glycerol + phosphate
-
 ?
3.1.3.2 alpha-glycerophosphate + H2O relative hydrolytic rate: 19% Labeo rohita glycerol + phosphate
-
 ?
3.1.3.2 alpha-naphthyl phosphate + H2O relative hydrolytic rate: 3% Labeo rohita 1-naphthol + phosphate
-
 ?
3.1.3.2 alpha-naphthyl phosphate + H2O relative hydrolytic rate: 8% Labeo rohita 1-naphthol + phosphate
-
 ?
3.1.3.2 alpha-naphthyl phosphate + H2O relative hydrolytic rate: 88% Labeo rohita 1-naphthol + phosphate
-
 ?
3.1.3.2 beta-glycerophosphate + H2O relative hydrolytic rate: 2% Labeo rohita glycerol + phosphate
-
 ?
3.1.3.2 beta-glycerophosphate + H2O relative hydrolytic rate: 70% Labeo rohita glycerol + phosphate
-
 ?
3.1.3.2 beta-glycerophosphate + H2O relative hydrolytic rate: 8% Labeo rohita glycerol + phosphate
-
 ?
3.1.3.2 beta-naphthyl phosphate + H2O relative hydrolytic rate: 116% Labeo rohita 2-naphthol + phosphate
-
 ?
3.1.3.2 beta-naphthyl phosphate + H2O relative hydrolytic rate: 70% Labeo rohita 2-naphthol + phosphate
-
 ?
3.1.3.2 beta-naphthyl phosphate + H2O relative hydrolytic rate: 81% Labeo rohita 2-naphthol + phosphate
-
 ?
3.1.3.2 cytidine monophosphate + H2O relative hydrolytic rate: 47% Labeo rohita cytidine + phosphate
-
 ?
3.1.3.2 D-glucose-1-phosphate + H2O relative hydrolytic rate: 13% Labeo rohita D-glucose + phosphate
-
 ?
3.1.3.2 D-glucose-6-phosphate + H2O relative hydrolytic rate: 14% Labeo rohita D-glucose + phosphate
-
 ?
3.1.3.2 flavin mononucleotide + H2O relative hydrolytic rate: 45% Labeo rohita riboflavin + phosphate
-
 ?
3.1.3.2 flavin mononucleotide + H2O relative hydrolytic rate: 91% Labeo rohita riboflavin + phosphate
-
 ?
3.1.3.2 flavin mononucleotide + H2O relative hydrolytic rate: 98% Labeo rohita riboflavin + phosphate
-
 ?
3.1.3.2 guanosine monophosphate + H2O relative hydrolytic rate: 32% Labeo rohita guanosine + phosphate
-
 ?
3.1.3.2 phenyl phosphate + H2O relative hydrolytic rate: 79% Labeo rohita phenol + phosphate
-
 ?
3.1.3.2 phenyl phosphate + H2O relative hydrolytic rate: 87% Labeo rohita phenol + phosphate
-
 ?
3.1.3.2 phenyl phosphate + H2O relative hydrolytic rate: 89% Labeo rohita phenol + phosphate
-
 ?
3.1.3.2 phosphotyrosine + H2O relative hydrolytic rate: 24% Labeo rohita tyrosine + phosphate
-
 ?
3.1.3.2 phosphotyrosine + H2O relative hydrolytic rate: 35% Labeo rohita tyrosine + phosphate
-
 ?
3.1.3.2 phosphotyrosine + H2O relative hydrolytic rate: 73% Labeo rohita tyrosine + phosphate
-
 ?
3.1.3.2 uridine monophosphate + H2O relative hydrolytic rate: 41% Labeo rohita uridine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 monomer 1 * 18000, isoenzyme 1, SDS-PAGE Labeo rohita
3.1.3.2 monomer 1 * 18000, isoenzyme 2, SDS-PAGE Labeo rohita

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 acid phosphatase
-
 Labeo rohita
3.1.3.2 high molecular weight acid phosphatase
-
 Labeo rohita
3.1.3.2 HM-ACP
-
 Labeo rohita
3.1.3.2 IF1 isoform 1 Labeo rohita
3.1.3.2 IF2 isoform 2 Labeo rohita
3.1.3.2 LM-ACP
-
 Labeo rohita
3.1.3.2 low molecular weight acid phosphatase
-
 Labeo rohita

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 37
-
 assay at Labeo rohita

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.5
-
 assay at Labeo rohita

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.2 0.0007
-
 vanadate
-
 Labeo rohita
3.1.3.2 0.0009
-
 molybdate
-
 Labeo rohita
3.1.3.2 0.013
-
 pyridoxamine 5'-phosphate pH 5.5, 37°C, isoenzyme 2 Labeo rohita
3.1.3.2 0.017
-
 pyridoxamine 5'-phosphate pH 5.5, 37°C, isoenzyme 1 Labeo rohita
3.1.3.2 0.02
-
 pyridoxal 5'-phosphate pH 5.5, 37°C, isoenzyme 2 Labeo rohita
3.1.3.2 0.024
-
 Tartrate
-
 Labeo rohita
3.1.3.2 0.025
-
 vanadate pH 5.5, 37°C, isoenzyme 2 Labeo rohita
3.1.3.2 0.08
-
 molybdate pH 5.5, 37°C, isoenzyme 2 Labeo rohita
3.1.3.2 0.138
-
 vanadate pH 5.5, 37°C, isoenzyme 1 Labeo rohita
3.1.3.2 0.2
-
 molybdate pH 5.5, 37°C, isoenzyme 1 Labeo rohita
3.1.3.2 0.208
-
 pyridoxal 5'-phosphate pH 5.5, 37°C, isoenzyme 1 Labeo rohita
3.1.3.2 1.4
-
 inorganic phosphate pH 5.5, 37°C, isoenzyme 2 Labeo rohita
3.1.3.2 1.5
-
 inorganic phosphate
-
 Labeo rohita
3.1.3.2 2.7
-
 inorganic phosphate pH 5.5, 37°C, isoenzyme 1 Labeo rohita