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Literature summary extracted from

  • Chu, X.; Yu, W.; Wu, L.; Liu, X.; Li, N.; Li, D.
    Effect of a disulfide bond on mevalonate kinase (2007), Biochim. Biophys. Acta, 1774, 1571-1581.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.36 A141C significantly higher thermal activity than wild-type Rattus norvegicus
2.7.1.36 C107A decrease in temperature stability, slight increase in Km value for ATP Methanocaldococcus jannaschii
2.7.1.36 C107S decrease in temperature stability, slight increase in Km value for ATP Methanocaldococcus jannaschii
2.7.1.36 C107S/C281S decrease in temperature stability, slight increase in Km value for ATP Methanocaldococcus jannaschii
2.7.1.36 C197A/C281A decrease in temperature stability, slight increase in Km value for ATP Methanocaldococcus jannaschii
2.7.1.36 C281A decrease in temperature stability, slight increase in Km value for ATP Methanocaldococcus jannaschii
2.7.1.36 C281S decrease in temperature stability, slight increase in Km value for ATP Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.035
-
 (R)-mevalonate wild-type, pH 7.5, 34°C Rattus norvegicus
2.7.1.36 0.061
-
 (R)-mevalonate mutant C281S, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 0.07
-
 (R)-mevalonate mutant C107A, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 0.071
-
 (R)-mevalonate mutant C107A/C281A, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 0.076
-
 (R)-mevalonate mutant C107S, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 0.106
-
 (R)-mevalonate mutant C281A, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 0.106
-
 (R)-mevalonate wild-type, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 0.111
-
 (R)-mevalonate mutant C107S/C281S, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 0.186
-
 (R)-mevalonate mutant A141C, pH 7.5, 34°C Rattus norvegicus
2.7.1.36 0.95
-
 ATP wild-type, pH 7.5, 34°C Rattus norvegicus
2.7.1.36 0.97
-
 ATP mutant A141C, pH 7.5, 34°C Rattus norvegicus
2.7.1.36 1.18
-
 ATP wild-type, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 1.58
-
 ATP mutant C107A/C281A, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 2.61
-
 ATP mutant C107A, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 2.76
-
 ATP mutant C281A, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 2.97
-
 ATP mutant C281S, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 3.11
-
 ATP mutant C107S, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 3.36
-
 ATP mutant C107S/C281S, pH 7.5, 34°C Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Methanocaldococcus jannaschii
-
-
-
2.7.1.36 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate
-
 Rattus norvegicus ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 ATP + (R)-mevalonate
-
 Methanocaldococcus jannaschii ADP + (R)-5-phosphomevalonate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.36 72
-
 wild-type Methanocaldococcus jannaschii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.36 34
-
 wild-type, 25% of maximal activity Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.36 44
-
 10 min, residual activity of wild-type 50%, of mutant A141C 85% Rattus norvegicus
2.7.1.36 47
-
 10 min, residual activity of wild-type 15%, of mutant A141C 55% Rattus norvegicus
2.7.1.36 83
-
 10 min, residual activity of wild-type 50%, mutants C107S and C281S 20%, mutant C107S/C281S 15%, mutants C107A and C281A 20%, mutant C107A/C281A 18% Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.36 3.1
-
 (R)-mevalonate mutant C107S/C281S, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 5.2
-
 (R)-mevalonate mutant A141C, pH 7.5, 34°C Rattus norvegicus
2.7.1.36 8.6
-
 (R)-mevalonate mutant C107A/C281A, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 10.5
-
 (R)-mevalonate mutant C107S, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 12.7
-
 (R)-mevalonate mutant C281S, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 17.1
-
 (R)-mevalonate mutant C107A, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 21.9
-
 (R)-mevalonate wild-type, pH 7.5, 34°C Rattus norvegicus
2.7.1.36 23.3
-
 (R)-mevalonate mutant C281A, pH 7.5, 34°C Methanocaldococcus jannaschii
2.7.1.36 28.5
-
 (R)-mevalonate wild-type, pH 7.5, 34°C Methanocaldococcus jannaschii