EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.36 | A141C | significantly higher thermal activity than wild-type | Rattus norvegicus |
2.7.1.36 | C107A | decrease in temperature stability, slight increase in Km value for ATP | Methanocaldococcus jannaschii |
2.7.1.36 | C107S | decrease in temperature stability, slight increase in Km value for ATP | Methanocaldococcus jannaschii |
2.7.1.36 | C107S/C281S | decrease in temperature stability, slight increase in Km value for ATP | Methanocaldococcus jannaschii |
2.7.1.36 | C197A/C281A | decrease in temperature stability, slight increase in Km value for ATP | Methanocaldococcus jannaschii |
2.7.1.36 | C281A | decrease in temperature stability, slight increase in Km value for ATP | Methanocaldococcus jannaschii |
2.7.1.36 | C281S | decrease in temperature stability, slight increase in Km value for ATP | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.36 | 0.035 | - |
(R)-mevalonate | wild-type, pH 7.5, 34°C | Rattus norvegicus | |
2.7.1.36 | 0.061 | - |
(R)-mevalonate | mutant C281S, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 0.07 | - |
(R)-mevalonate | mutant C107A, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 0.071 | - |
(R)-mevalonate | mutant C107A/C281A, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 0.076 | - |
(R)-mevalonate | mutant C107S, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 0.106 | - |
(R)-mevalonate | mutant C281A, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 0.106 | - |
(R)-mevalonate | wild-type, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 0.111 | - |
(R)-mevalonate | mutant C107S/C281S, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 0.186 | - |
(R)-mevalonate | mutant A141C, pH 7.5, 34°C | Rattus norvegicus | |
2.7.1.36 | 0.95 | - |
ATP | wild-type, pH 7.5, 34°C | Rattus norvegicus | |
2.7.1.36 | 0.97 | - |
ATP | mutant A141C, pH 7.5, 34°C | Rattus norvegicus | |
2.7.1.36 | 1.18 | - |
ATP | wild-type, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 1.58 | - |
ATP | mutant C107A/C281A, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 2.61 | - |
ATP | mutant C107A, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 2.76 | - |
ATP | mutant C281A, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 2.97 | - |
ATP | mutant C281S, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 3.11 | - |
ATP | mutant C107S, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 3.36 | - |
ATP | mutant C107S/C281S, pH 7.5, 34°C | Methanocaldococcus jannaschii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.36 | Methanocaldococcus jannaschii | - |
- |
- |
2.7.1.36 | Rattus norvegicus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.36 | ATP + (R)-mevalonate | - |
Rattus norvegicus | ADP + (R)-5-phosphomevalonate | - |
? | |
2.7.1.36 | ATP + (R)-mevalonate | - |
Methanocaldococcus jannaschii | ADP + (R)-5-phosphomevalonate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.36 | 72 | - |
wild-type | Methanocaldococcus jannaschii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.36 | 34 | - |
wild-type, 25% of maximal activity | Methanocaldococcus jannaschii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.36 | 44 | - |
10 min, residual activity of wild-type 50%, of mutant A141C 85% | Rattus norvegicus |
2.7.1.36 | 47 | - |
10 min, residual activity of wild-type 15%, of mutant A141C 55% | Rattus norvegicus |
2.7.1.36 | 83 | - |
10 min, residual activity of wild-type 50%, mutants C107S and C281S 20%, mutant C107S/C281S 15%, mutants C107A and C281A 20%, mutant C107A/C281A 18% | Methanocaldococcus jannaschii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.36 | 3.1 | - |
(R)-mevalonate | mutant C107S/C281S, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 5.2 | - |
(R)-mevalonate | mutant A141C, pH 7.5, 34°C | Rattus norvegicus | |
2.7.1.36 | 8.6 | - |
(R)-mevalonate | mutant C107A/C281A, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 10.5 | - |
(R)-mevalonate | mutant C107S, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 12.7 | - |
(R)-mevalonate | mutant C281S, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 17.1 | - |
(R)-mevalonate | mutant C107A, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 21.9 | - |
(R)-mevalonate | wild-type, pH 7.5, 34°C | Rattus norvegicus | |
2.7.1.36 | 23.3 | - |
(R)-mevalonate | mutant C281A, pH 7.5, 34°C | Methanocaldococcus jannaschii | |
2.7.1.36 | 28.5 | - |
(R)-mevalonate | wild-type, pH 7.5, 34°C | Methanocaldococcus jannaschii |