EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.6 | K+ | K+-activated type II enzyme: binding of K+ is required to enhance activity through induced conformational change | Lactococcus lactis | |
2.4.2.6 | additional information | does not require phosphate derivatives such as ATP, thiamine diphosphate, pyridoxal phosphate to complete 2-deoxyribose transfer | Lactococcus lactis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.6 | in pET-22b(+) for expression in Escherichia coli BL21(DE3) | Lactococcus lactis |
2.4.2.6 | in pGEM-T for cloning | Lactococcus lactis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.6 | Ca2+ | inhibits base exchange activity at 1 mM | Lactococcus lactis | |
2.4.2.6 | CO2 | reduces base exchange activity by 87-99% at 1 mM | Lactococcus lactis | |
2.4.2.6 | Cu2+ | reduces base exchange activity by 87-99% at 1 mM | Lactococcus lactis | |
2.4.2.6 | Mg2+ | inhibits base exchange activity at 1mM | Lactococcus lactis | |
2.4.2.6 | Mn2+ | reduces base exchange activity by 87-99% at 1 mM | Lactococcus lactis | |
2.4.2.6 | Zn2+ | reduces base exchange activity by 87-99% at 1 mM | Lactococcus lactis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.6 | K+ | enhances base exchange activity and thermostability, 1.66-fold activation by 1 mM KCl (reaches plateau at 20 mM) | Lactococcus lactis | |
2.4.2.6 | additional information | enzymatic activity depends on monovalent cations as cofactors rather than catalysts | Lactococcus lactis | |
2.4.2.6 | additional information | K+ and NH4+ have no synergistic effects | Lactococcus lactis | |
2.4.2.6 | additional information | Li+ and Na+ do not activate | Lactococcus lactis | |
2.4.2.6 | NH4+ | enhances base exchange activity and thermostability, 1.43-fold activation by 1 mM (NH4)2SO4 (reaches plateau at 20 mM) | Lactococcus lactis | |
2.4.2.6 | Rb+ | enhances base exchange activity, 1.51-fold activation by 1 mM RbCl | Lactococcus lactis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 17250 | - |
4 * 17250, elution as single peak from the preparative gel filtration column | Lactococcus lactis |
2.4.2.6 | 18290 | - |
theoretical molecular weight | Lactococcus lactis |
2.4.2.6 | 25500 | - |
according to migration in SDS-12.5% polyacrylamide gel electrophoresis | Lactococcus lactis |
2.4.2.6 | 69000 | - |
gel filtration chromatography | Lactococcus lactis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.6 | additional information | Lactococcus lactis | preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed | ? | - |
? | |
2.4.2.6 | purine base + 2-deoxy-D-ribosyl-base | Lactococcus lactis | - |
purine deoxyribonucleoside + base | preferred over pyrimidine deoxyribonucleoside synthesis | r | |
2.4.2.6 | pyrimidine base + 2-deoxy-D-ribosyl-base | Lactococcus lactis | - |
pyrimidine deoxyribonucleoside + base | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.6 | Lactococcus lactis | - |
subsp. lactis JCM5808 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.6 | preclearing of bacterial lysate with 40% ammonium sulfate and subsequent precipitation of proteins with 90% ammonium sulfate, gel filtration of dissolved protein on Sephacryl S-200 column, combination of fractions with N-deoxyribosyltransferase activity, precipitation of proteins with 90% ammonium sulfate, dialysis of dissolved proteins and concentration by ultrafiltration, second gel filtration on Sephacryl S-200 column, combination of active fractions and concentration by ultrafiltration, recovery of 70.4% of activity, storage at -20°C in 50% glycerol | Lactococcus lactis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | additional information | - |
0.9% in presence of 1 mM ZnCl2 | Lactococcus lactis |
2.4.2.6 | additional information | - |
102% in presence of 1 mM NaCl | Lactococcus lactis |
2.4.2.6 | additional information | - |
105% in presence of 1 mM CsCl | Lactococcus lactis |
2.4.2.6 | additional information | - |
106% in presence of 1 mM NaSO4 | Lactococcus lactis |
2.4.2.6 | additional information | - |
12.9% in presence of 1 mM CoCl2 | Lactococcus lactis |
2.4.2.6 | additional information | - |
143% in presence of 1 mM NH4Cl | Lactococcus lactis |
2.4.2.6 | additional information | - |
151% in presence of 1 mM RbCl | Lactococcus lactis |
2.4.2.6 | additional information | - |
163% in presence of 1 mM (NH4)2SO4 | Lactococcus lactis |
2.4.2.6 | additional information | - |
165% in presence of 1 mM K2SO4 | Lactococcus lactis |
2.4.2.6 | additional information | - |
166% in presence of 1 mM (NH4)2SO4 and 1 mM KCl | Lactococcus lactis |
2.4.2.6 | additional information | - |
166% in presence of 1 mM KCl | Lactococcus lactis |
2.4.2.6 | additional information | - |
192% in presence of 10 mM KCl | Lactococcus lactis |
2.4.2.6 | additional information | - |
194% in presence of 10 mM (NH4)2SO4 | Lactococcus lactis |
2.4.2.6 | additional information | - |
3.2% in presence of 1 mM CuSO4 | Lactococcus lactis |
2.4.2.6 | additional information | - |
51.8% in presence of 1 mM CaCl2 | Lactococcus lactis |
2.4.2.6 | additional information | - |
66.3% in presence of 1 mM MgCl2 | Lactococcus lactis |
2.4.2.6 | additional information | - |
66% in presence of 1 mM MgSO4 | Lactococcus lactis |
2.4.2.6 | additional information | - |
68.3% in presence of 1 mM FeCl2 | Lactococcus lactis |
2.4.2.6 | additional information | - |
97.7% in presence of 1 mM LiCl | Lactococcus lactis |
2.4.2.6 | 6.67 | - |
equals 100%, purified protein, in presence of 0.5 mg/ml bovine serum albumin, pH 7.5, 10 min, 25°C | Lactococcus lactis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.6 | adenine + deoxycytidine | 64.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyadenosine + cytosine | - |
r | |
2.4.2.6 | adenine + deoxyguanosine | 46.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyadenosine + guanine | - |
r | |
2.4.2.6 | adenine + deoxyinosine | 57.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyadenosine + hypoxanthine | - |
r | |
2.4.2.6 | adenine + deoxyuridine | 75.4% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyadenosine + uracil | - |
r | |
2.4.2.6 | adenine + thymidine | 74.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyadenosine + thymine | - |
r | |
2.4.2.6 | cytosine + deoxyadenosine | 27.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxycytidine + adenine | - |
r | |
2.4.2.6 | cytosine + deoxyguanosine | 25.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxycytidine + guanine | - |
r | |
2.4.2.6 | cytosine + deoxyinosine | 32.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxycytidine + hypoxanthine | - |
r | |
2.4.2.6 | cytosine + deoxyuridine | 62.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxycytidine + uracil | - |
r | |
2.4.2.6 | cytosine + thymidine | 53.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxycytidine + thymine | - |
r | |
2.4.2.6 | cytosine + thymidine | standard reaction for cofactor analyses as well as activating compound and inhibitor studies, in presence of 40 mM ammonium sulfate and 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxycytidine + thymine | - |
r | |
2.4.2.6 | hypoxanthine + deoxyadenosine | 38.0% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyinosine + adenine | - |
r | |
2.4.2.6 | hypoxanthine + deoxycytidine | 51.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyinosine + cytosine | - |
r | |
2.4.2.6 | hypoxanthine + deoxyguanosine | 25.2% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyinosine + guanine | - |
r | |
2.4.2.6 | hypoxanthine + deoxyuridine | 65.6% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyinosine + uracil | - |
r | |
2.4.2.6 | hypoxanthine + thymidine | 76.8% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyinosine + thymine | - |
r | |
2.4.2.6 | additional information | preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed | Lactococcus lactis | ? | - |
? | |
2.4.2.6 | additional information | ribonucleoside, arabinosylnucleoside and 2,3-dideoxyribonucleoside are no substrates for base-exchange by YejD | Lactococcus lactis | ? | - |
? | |
2.4.2.6 | purine base + 2-deoxy-D-ribosyl-base | - |
Lactococcus lactis | purine deoxyribonucleoside + base | preferred over pyrimidine deoxyribonucleoside synthesis | r | |
2.4.2.6 | pyrimidine base + 2-deoxy-D-ribosyl-base | - |
Lactococcus lactis | pyrimidine deoxyribonucleoside + base | - |
r | |
2.4.2.6 | thymine + deoxyadenosine | 22.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | thymidine + adenine | - |
r | |
2.4.2.6 | thymine + deoxycytidine | 38.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | thymidine + cytosine | - |
r | |
2.4.2.6 | thymine + deoxyguanosine | 17.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | thymidine + guanine | - |
r | |
2.4.2.6 | thymine + deoxyinosine | 19.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin, equilibrium reached after 2 h | Lactococcus lactis | thymidine + hypoxanthine | - |
r | |
2.4.2.6 | thymine + deoxyuridine | 53.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | thymidine + uracil | - |
r | |
2.4.2.6 | uracil + deoxyadenosine | 17.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyuridine + adenine | - |
r | |
2.4.2.6 | uracil + deoxycytidine | 37.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyuridine + cytosine | - |
r | |
2.4.2.6 | uracil + deoxyguanosine | 16.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyuridine + guanine | - |
r | |
2.4.2.6 | uracil + deoxyinosine | 23.8% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyuridine + hypoxanthine | - |
r | |
2.4.2.6 | uracil + thymidine | 43.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin | Lactococcus lactis | deoxyuridine + thymine | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.6 | homotetramer | 4 * 17250, elution as single peak from the preparative gel filtration column | Lactococcus lactis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.6 | N-deoxyribosyltransferase | - |
Lactococcus lactis |
2.4.2.6 | NTD | - |
Lactococcus lactis |
2.4.2.6 | nucleoside N-deoxyribosyltransferase | - |
Lactococcus lactis |
2.4.2.6 | trans-N-deoxyribosylase | - |
Lactococcus lactis |
2.4.2.6 | YejD | - |
Lactococcus lactis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 30 | - |
assay at, pH 7.5 | Lactococcus lactis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | additional information | - |
(NH4)2SO4 is more thermostabilizing than KCl | Lactococcus lactis |
2.4.2.6 | 25 | - |
pH 7.5, 20 min, 60% activity lost, in presence of 10 mM (NH4)2SO4 only 20% activity lost, in presence of 500 mM KCL only 6% activity lost | Lactococcus lactis |
2.4.2.6 | 30 | - |
pH 7.5, 20 min, in presence of 500 mM (NH4)2SO4 fully active | Lactococcus lactis |
2.4.2.6 | 35 | - |
and more, pH 7.5, 20 min, 75% activity lost | Lactococcus lactis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 7.5 | - |
assay at | Lactococcus lactis |