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Literature summary extracted from

  • Reger, A.S.; Wu, R.; Dunaway-Mariano, D.; Gulick, A.M.
    Structural characterization of a 140 degrees domain movement in the two-step reaction catalyzed by 4-chlorobenzoate:CoA ligase (2008), Biochemistry, 47, 8016-8025.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.2.1.33 purified recombinant CBL bound to 4-chlorobenzoyl-AMP, hanging drop vapor diffusion at 4°C using 16-24% pentaerythritol propoxylate 426, and 0.1 M K+-HEPES, pH 6.5 or 6.75, and optimized via hanging drop vapor diffusion using 14-18% PEG 1000, 50-100 mM magnesium nitrate and 100 mM MOPS, pH 7.0, the cryoprotectant contains 24% pentaerythritol propoxylate 426, 24% ethylene glycol, and 0.1 M K+-HEPES, 1 mM ATP, and 1 mM 4-chlorobenzoate, X-ray diffraction structure determination and analysis at 2.0-2.25 A resolution, molecular replacement Alcaligenes sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.33 4-chlorophenacyl-CoA the product analogue traps the enzyme in the thioester-forming conformation, binding structure, overview Alcaligenes sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.33 Mg2+
-
 Alcaligenes sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.33 4-Chlorobenzoate + CoA + ATP Alcaligenes sp.
-
 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.33 Alcaligenes sp. Q8GN86
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.2.1.33 4-Chlorobenzoate + CoA + ATP = 4-chlorobenzoyl-CoA + AMP + diphosphate the enzyme alternates between two conformations during catalysis of the two half-reactions, structure-function analysis, domain alternation strategy, overview Alcaligenes sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.33 4-Chlorobenzoate + CoA + ATP
-
 Alcaligenes sp. 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.33 More determination and analysis of the structure of CBL in the adenylate forming conformation bound to 4-chlorobenzoate and the adenylate-forming conformation bound to the adenylate intermediate, overview Alcaligenes sp.

Synonyms

EC Number Synonyms Comment Organism
6.2.1.33 4-chlorobenzoate:CoA ligase
-
 Alcaligenes sp.
6.2.1.33 CBL
-
 Alcaligenes sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.33 25
-
 assay at Alcaligenes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.33 7.5
-
 assay at Alcaligenes sp.

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.33 ATP
-
 Alcaligenes sp.