EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.19 | restrained-based, molecular modelling; open conformation model of apoenzyme generated based on coordinates from PDB: 2BJB (MtEPSPS), closed conformation model of enzyme/3-phosphoshikimate/glyphosate complex generated based on PDB: 1G6S | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.19 | glyphosate | Kd: 350 micromolar (pH 6.9, 25°C), binding induces conformational change that leads to increase in alpha helix (40% to 57%) and decrease in beta-sheet (30% to 23%) content (revealed by circular dichroism spectroscopy) and prevents access of solvent to core molecule (revealed by hydrogen/deuterium exchange, peptic digestion and LC-ESI-MS) | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.19 | 46430 | - |
by ESI-MS | Mycobacterium tuberculosis |
2.5.1.19 | 46430 | - |
deduced from primary structure | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.19 | Mycobacterium tuberculosis | P9WPY5 | - |
- |
2.5.1.19 | Mycobacterium tuberculosis H37Rv | P9WPY5 | - |
- |
EC Number | Renatured (Comment) | Organism |
---|---|---|
2.5.1.19 | pellet from 80% (w/v) ammonium sulfate solution, resolubilization and dialysis against 5 mM sodium phosphate, pH 6.9 | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.19 | Mt EPSPS | - |
Mycobacterium tuberculosis |
2.5.1.19 | Mycobacterium tuberculosis 5-enolpyruvylshikimate-3-phosphate synthase | - |
Mycobacterium tuberculosis |