EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.6.1.13 | phosphatidylcholine | - |
Bacillus thuringiensis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.11 | expressed in Escherichia coli BL21-Codonplus (DE3)-RIL cell | Bacillus thuringiensis |
4.6.1.13 | transformed into Escherichia coli BL21-Codonplus (DE3)-RIL cells | Bacillus thuringiensis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.4.11 | G48A | single PI-PLC mutant, site-directed mutagenesis | Bacillus thuringiensis |
3.1.4.11 | I43A | single PI-PLC mutant, site-directed mutagenesis | Bacillus thuringiensis |
3.1.4.11 | I43W | single PI-PLC mutant, site-directed mutagenesis | Bacillus thuringiensis |
3.1.4.11 | K44E | single PI-PLC mutant, site-directed mutagenesis | Bacillus thuringiensis |
3.1.4.11 | L39A | single PI-PLC mutant, site-directed mutagenesis | Bacillus thuringiensis |
3.1.4.11 | P42G | single PI-PLC mutant, site-directed mutagenesis | Bacillus thuringiensis |
3.1.4.11 | Q45A | single PI-PLC mutant, site-directed mutagenesis | Bacillus thuringiensis |
3.1.4.11 | V46A | single PI-PLC mutant, site-directed mutagenesis | Bacillus thuringiensis |
3.1.4.11 | W47A/W242A | mutant is unable to bind to PC | Bacillus thuringiensis |
4.6.1.13 | G48A | single mutant | Bacillus thuringiensis |
4.6.1.13 | I43A | single mutant | Bacillus thuringiensis |
4.6.1.13 | I43W | single mutant | Bacillus thuringiensis |
4.6.1.13 | I43W/W47I | are made by introducing the second mutation in the gene coding for a single mutant | Bacillus thuringiensis |
4.6.1.13 | K44E | single mutant | Bacillus thuringiensis |
4.6.1.13 | L39A | single mutant | Bacillus thuringiensis |
4.6.1.13 | L39A/V46A | are made by introducing the second mutation in the gene coding for a single mutant | Bacillus thuringiensis |
4.6.1.13 | P42G | single mutant | Bacillus thuringiensis |
4.6.1.13 | Q45A | single mutant | Bacillus thuringiensis |
4.6.1.13 | V46A | single mutant | Bacillus thuringiensis |
4.6.1.13 | W47A/W242A | dimeric mutant, which is unable to bind to phosphatidycholine | Bacillus thuringiensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.11 | Bacillus thuringiensis | - |
- |
- |
4.6.1.13 | Bacillus thuringiensis | P08954 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.6.1.13 | Q-sepharose fast-flow column and phenyl-Sepharose column, monitored by SDS-PAGE | Bacillus thuringiensis |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.4.11 | dimer | - |
Bacillus thuringiensis |
4.6.1.13 | monomer | although small amounts of dimer have been noted previously | Bacillus thuringiensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.11 | phosphatidylinositol-specific phospholipase C | - |
Bacillus thuringiensis |
3.1.4.11 | PI-PLC | - |
Bacillus thuringiensis |
4.6.1.13 | phosphatidylinositol-specific phospholipase C | - |
Bacillus thuringiensis |
4.6.1.13 | PI-PLC | - |
Bacillus thuringiensis |