Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Guo, S.; Zhang, X.; Seaton, B.A.; Roberts, M.F.
    Role of helix B residues in interfacial activation of a bacterial phosphatidylinositol-specific phospholipase C (2008), Biochemistry, 47, 4201-4210.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.13 phosphatidylcholine
-
Bacillus thuringiensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.11 expressed in Escherichia coli BL21-Codonplus (DE3)-RIL cell Bacillus thuringiensis
4.6.1.13 transformed into Escherichia coli BL21-Codonplus (DE3)-RIL cells Bacillus thuringiensis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.11 G48A single PI-PLC mutant, site-directed mutagenesis Bacillus thuringiensis
3.1.4.11 I43A single PI-PLC mutant, site-directed mutagenesis Bacillus thuringiensis
3.1.4.11 I43W single PI-PLC mutant, site-directed mutagenesis Bacillus thuringiensis
3.1.4.11 K44E single PI-PLC mutant, site-directed mutagenesis Bacillus thuringiensis
3.1.4.11 L39A single PI-PLC mutant, site-directed mutagenesis Bacillus thuringiensis
3.1.4.11 P42G single PI-PLC mutant, site-directed mutagenesis Bacillus thuringiensis
3.1.4.11 Q45A single PI-PLC mutant, site-directed mutagenesis Bacillus thuringiensis
3.1.4.11 V46A single PI-PLC mutant, site-directed mutagenesis Bacillus thuringiensis
3.1.4.11 W47A/W242A mutant is unable to bind to PC Bacillus thuringiensis
4.6.1.13 G48A single mutant Bacillus thuringiensis
4.6.1.13 I43A single mutant Bacillus thuringiensis
4.6.1.13 I43W single mutant Bacillus thuringiensis
4.6.1.13 I43W/W47I are made by introducing the second mutation in the gene coding for a single mutant Bacillus thuringiensis
4.6.1.13 K44E single mutant Bacillus thuringiensis
4.6.1.13 L39A single mutant Bacillus thuringiensis
4.6.1.13 L39A/V46A are made by introducing the second mutation in the gene coding for a single mutant Bacillus thuringiensis
4.6.1.13 P42G single mutant Bacillus thuringiensis
4.6.1.13 Q45A single mutant Bacillus thuringiensis
4.6.1.13 V46A single mutant Bacillus thuringiensis
4.6.1.13 W47A/W242A dimeric mutant, which is unable to bind to phosphatidycholine Bacillus thuringiensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.11 Bacillus thuringiensis
-
-
-
4.6.1.13 Bacillus thuringiensis P08954
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.13 Q-sepharose fast-flow column and phenyl-Sepharose column, monitored by SDS-PAGE Bacillus thuringiensis

Subunits

EC Number Subunits Comment Organism
3.1.4.11 dimer
-
Bacillus thuringiensis
4.6.1.13 monomer although small amounts of dimer have been noted previously Bacillus thuringiensis

Synonyms

EC Number Synonyms Comment Organism
3.1.4.11 phosphatidylinositol-specific phospholipase C
-
Bacillus thuringiensis
3.1.4.11 PI-PLC
-
Bacillus thuringiensis
4.6.1.13 phosphatidylinositol-specific phospholipase C
-
Bacillus thuringiensis
4.6.1.13 PI-PLC
-
Bacillus thuringiensis