Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Calvin, K.; Xue, S.; Ellis, C.; Mitchell, M.H.; Li, H.
    Probing the catalytic triad of an archaeal RNA splicing endonuclease (2008), Biochemistry, 47, 13659-13665.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.16
-
Archaeoglobus fulgidus

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.16 H257A residue of the catalytic triad, 28-fold reduction in activity Archaeoglobus fulgidus
4.6.1.16 H257C residue of the catalytic triad Archaeoglobus fulgidus
4.6.1.16 H257D residue of the catalytic triad Archaeoglobus fulgidus
4.6.1.16 H257N residue of the catalytic triad Archaeoglobus fulgidus
4.6.1.16 K287E residue of the catalytic triad, no activity in absence of divalent metal ions, activity observed in the presence of specific divalent metal ions Archaeoglobus fulgidus
4.6.1.16 K287Q residue of the catalytic triad Archaeoglobus fulgidus
4.6.1.16 K287R residue of the catalytic triad Archaeoglobus fulgidus
4.6.1.16 Y246F residue of the catalytic triad, 7-fold reduction in activity Archaeoglobus fulgidus
4.6.1.16 Y246F/H257A residues of the catalytic triad, no activity Archaeoglobus fulgidus
4.6.1.16 Y246H residue of the catalytic triad Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.16 0.00025
-
pretRNA wild-type protein, substrate: 5'-AAGCGACCGACC*AUA*GCUGCA-3' or 5'-AAUGCAGCGGUC*AAA*GGUCGC-3' Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.16 Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.16
-
Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.16 5'-AAGCGACCGACC*AUA*GCUGCA-3' + H2O
-
Archaeoglobus fulgidus ?
-
?
4.6.1.16 5'-AAUGCAGCGGUC*AAA*GGUCGC-3' + H2O
-
Archaeoglobus fulgidus ?
-
?
4.6.1.16 pretRNA
-
Archaeoglobus fulgidus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.16 RNA splicing endonuclease
-
Archaeoglobus fulgidus