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Literature summary extracted from

  • Woycechowsky, K.J.; Choutko, A.; Vamvaca, K.; Hilvert, D.
    Relative tolerance of an enzymatic molten globule and its thermostable counterpart to point mutation (2008), Biochemistry, 47, 13489-13496.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.5 expressed in Escherichia coli KA13 cells Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.5 D48G the mutation causes kcat/Km to decrease by 3 fold Methanocaldococcus jannaschii
5.4.99.5 F77W mutant exhibits decreased activity compared to the wild type enzyme Methanocaldococcus jannaschii
5.4.99.5 Q88N mutant exhibits decreased activity compared to the wild type enzyme Methanocaldococcus jannaschii
5.4.99.5 R51Q mutant exhibits decreased activity compared to the wild type enzyme Methanocaldococcus jannaschii
5.4.99.5 V35A mutant exhibits decreased activity compared to the wild type enzyme Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.5 0.03
-
 chorismate mutant enzyme V35A, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
5.4.99.5 0.054
-
 chorismate wild type enzyme, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
5.4.99.5 0.059
-
 chorismate mutant enzyme D48G, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
5.4.99.5 0.073
-
 chorismate mutant enzyme F77W, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
5.4.99.5 1.2
-
 chorismate mutant enzyme Q88N, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
5.4.99.5 1.3
-
 chorismate mutant enzyme R51Q, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.5 Ni2+ affinity column chromatography and gel filtration Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate
-
 Methanocaldococcus jannaschii Prephenate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.5 dimer
-
 Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.5 0.042
-
 chorismate mutant enzyme Q88N, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
5.4.99.5 0.41
-
 chorismate mutant enzyme F77W, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
5.4.99.5 0.5
-
 chorismate mutant enzyme V35A, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
5.4.99.5 1.3
-
 chorismate mutant enzyme D48G, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
5.4.99.5 2.6
-
 chorismate mutant enzyme R51Q, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
5.4.99.5 3
-
 chorismate wild type enzyme, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii