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Literature summary extracted from

  • Tilley, D.M.; Evans, C.R.; Larson, T.M.; Edwards, K.A.; Friesen, J.A.
    Identification and characterization of the nuclear isoform of Drosophila melanogaster CTP:phosphocholine cytidylyltransferase (2008), Biochemistry, 47, 11838-11846.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.15 diphosphatidylglycerol
-
 Drosophila melanogaster
2.7.7.15 additional information phosphatidylethanolamine and phosphatidic acid do not have a significant effect on CCT1 activity Drosophila melanogaster
2.7.7.15 oleate
-
 Drosophila melanogaster
2.7.7.15 phosphatidylcholine 6fold enhancement of activity at 0.025 mM Drosophila melanogaster
2.7.7.15 phosphatidylglycerol
-
 Drosophila melanogaster
2.7.7.15 phosphatidylinositol
-
 Drosophila melanogaster
2.7.7.15 phosphatidylserine
-
 Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.15 expressed in Sf9 cells and Schneider 2 cells Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.15 0.63
-
 choline phosphate isozyme CCT2 Drosophila melanogaster
2.7.7.15 0.81
-
 CTP isozyme CCT2 Drosophila melanogaster
2.7.7.15 1.21
-
 CTP isozyme CCT1 Drosophila melanogaster
2.7.7.15 2.29
-
 choline phosphate isozyme CCT1 Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.15 cytoplasm isozyme CCT2 Drosophila melanogaster 5737
-
2.7.7.15 nucleus isozyme CCT1 Drosophila melanogaster 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.15 Ca2+ Ca2+ yields approximately 9% of the activity seen with Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ Drosophila melanogaster
2.7.7.15 Co2+ Co2+ results in significant activity at a concentration of 25 mM, with 41% of the activity seen with 25 mM Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ Drosophila melanogaster
2.7.7.15 Mg2+ the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ Drosophila melanogaster
2.7.7.15 Mn2+ Mn2+ results in significant activity at a concentration of 25 mM, with 51% of the activity seen with 25 mM Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ Drosophila melanogaster
2.7.7.15 Ni2+ Ni2+ yields approximately 9% of the activity seen with Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ Drosophila melanogaster
2.7.7.15 Zn2+ Zn2+ results in catalytic activity of only 1% when compared to the activity in the presence of Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.15 Drosophila melanogaster Q7K4C7
-
-
2.7.7.15 Drosophila melanogaster Q9W0D9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.15 Talon metal affinity column chromatography Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.15 CTP + choline phosphate
-
 Drosophila melanogaster diphosphate + CDP-choline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.15 CCT1 isozyme Drosophila melanogaster
2.7.7.15 CCT2 isozyme Drosophila melanogaster
2.7.7.15 CTP:phosphocholine cytidylyltransferase
-
 Drosophila melanogaster