Literature summary extracted from
Amero, C.D.; Boomershine, W.P.; Xu, Y.; Foster, M.
Solution structure of Pyrococcus furiosus RPP21, a component of the archaeal RNase P holoenzyme, and interactions with its RPP29 protein partner (2008), Biochemistry, 47, 11704-11710.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.26.5 |
expressed in Escherichia coli Rosetta BL21(DE3) cells |
Pyrococcus furiosus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.26.5 |
Pyrococcus furiosus |
Q8U0H6 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.26.5 |
HiTrap column chromatography and C4 reversed-phase high performance liquid chromatography |
Pyrococcus furiosus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.26.5 |
pre-tRNA precursor + H2O |
cleaves the 5'-leader sequence of precursor tRNAs during their maturation |
Pyrococcus furiosus |
tRNA + 5'-oligoribonucleotide |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.26.5 |
RNase P |
the catalytic RNA subunit RPR of ribonuclease P is associated with four protein subunits homologous to four eukaryotic nuclear RNase P proteins: RPP21, RPP29, RPP30, and POP5 |
Pyrococcus furiosus |