Literature summary extracted from
Bender, G.; Poyner, R.R.; Reed, G.H.
Identification of the substrate radical intermediate derived from ethanolamine during catalysis by ethanolamine ammonia-lyase (2008), Biochemistry, 47, 11360-11366.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.3.1.7 |
overexpression in Escherichia coli |
Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.3.1.7 |
hydroxyethylhydrazine |
the latter suicide inhibitor effects a stoichiometric conversion of enzyme-bound adenosylcobalamin into its cleaved form cob(II)alamin |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.3.1.7 |
Co2+ |
included in the cofactor B12 |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.3.1.7 |
31000 |
- |
beta-subunit |
Salmonella enterica subsp. enterica serovar Typhimurium |
4.3.1.7 |
50000 |
- |
alpha-subunit |
Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.3.1.7 |
ethanolamine |
Salmonella enterica subsp. enterica serovar Typhimurium |
elimination of ammonia from the vicinal position of short chain amino alcohol to give the corresponding oxo product |
acetaldehyde + NH3 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.3.1.7 |
Salmonella enterica subsp. enterica serovar Typhimurium |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.3.1.7 |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
4.3.1.7 |
additional information |
- |
specific activity is 30-45 micromol ethanolamine/min/mg of EAL at 25°C, determined by the EAL-alcohol dehydrogenase coupled assay |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.3.1.7 |
ethanolamine |
elimination of ammonia from the vicinal position of short chain amino alcohol to give the corresponding oxo product |
Salmonella enterica subsp. enterica serovar Typhimurium |
acetaldehyde + NH3 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.3.1.7 |
hexamer |
functional protein is a hexamer of alpha-beta-dimers (alpha-subunit of 50000 Da and beta-subunit of 31000 Da) |
Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.3.1.7 |
EAL |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
4.3.1.7 |
ethanolamine ammonia-lyase |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.3.1.7 |
7.5 |
- |
assay at |
Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
4.3.1.7 |
coenzyme B12 |
adenosylcobalamin, 70% of the enzyme-bound adenosylcobalamin is cleaved to cob(2)-alamin in the steady state of the reaction |
Salmonella enterica subsp. enterica serovar Typhimurium |
|