EC Number | Cloned (Comment) | Organism |
---|---|---|
2.2.1.1 | plasmid pGSJ427 carrying the gene for TKA/His6-tag expressed in Escherichia coli strain JM109 | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.2.1.1 | hanging drop vapor diffusion method, transketolase in a covalent complex with donor ketoses D-xylulose 5-phosphate and D-fructose 6-phosphate at 1.47 A and 1.65 A resolution, reveal significant strain in the tetrahedral cofactor-sugar adducts with a 25-30° out-of-plane distortion of the C2-Calpha bond connecting the carbonyl of the substrates with the C2 of the cofactors thiazolium part. The noncovalent complex with acceptor aldose ribose 5-phosphate reveals that the sugar is present in multiple forms, in a strained ring-closed beta-D-furanose form in C2-exo conformation as well as in an extended acyclic aldehyde form, with the reactive C1 aldo function held close to Calpha of the presumably planar carbanion/enamine intermediate | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.2.1.1 | H26A/H261A | three hydrogen-bonding interactions between the active site and the 3-hydroxyl and 4-hydroxyl groups of the intermediate cannot be formed when compared to the wild-type | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.1 | Ca2+ | bound at the subunit interface | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.2.1.1 | Escherichia coli | P27302 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.1 | D-fructose 6-phosphate + D-ribose 5-phosphate | - |
Escherichia coli | D-erythrose 4-phosphate + sedoheptulose 7-phosphate | - |
? | |
2.2.1.1 | D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Escherichia coli | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.2.1.1 | TKA | - |
Escherichia coli |
2.2.1.1 | transketolase A | - |
Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.1 | thiamine diphosphate | bound at the subunit interface | Escherichia coli |