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Literature summary extracted from

  • Canales, J.; Fernandez, A.; Ribeiro, J.M.; Cabezas, A.; Rodrigues, J.R.; Cameselle, J.C.; Costas, M.J.
    Mn2+-dependent ADP-ribose/CDP-alcohol pyrophosphatase: a novel metallophosphoesterase family preferentially expressed in rodent immune cells (2008), Biochem. J., 413, 103-113.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.53 expressed in Escherichia coli BL21 cells Rattus norvegicus
3.6.1.53 expression as GST-fusion protein Rattus norvegicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.53 homology modeling based on crystal structure of the Danio rerio protein, Protein Data Base entry 2NXF, and docking of ADP-ribose Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.53 additional information incubation with 2.5 mM EDTA does not affect ADPRibase-Mn activity; not inhibitory: EDTA up to 2.5 mM Rattus norvegicus
3.6.1.53 o-phenanthroline 5 mM causes near full inactivation in 1.5 h Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.53 0.039
-
ADP-ribose pH 7.5, 37°C Rattus norvegicus
3.6.1.53 0.039
-
ADP-ribose recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C Rattus norvegicus
3.6.1.53 0.201
-
ADP pH 7.5, 37°C Rattus norvegicus
3.6.1.53 0.201
-
ADP recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C Rattus norvegicus
3.6.1.53 0.3
-
CDP-choline pH 7.5, 37°C Rattus norvegicus
3.6.1.53 0.3
-
CDP-choline recombinant, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C Rattus norvegicus
3.6.1.53 0.304
-
CDP-glycerol pH 7.5, 37°C Rattus norvegicus
3.6.1.53 0.304
-
CDP-glycerol recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C Rattus norvegicus
3.6.1.53 1.422
-
CDP-ethanolamine pH 7.5, 37°C Rattus norvegicus
3.6.1.53 1.422
-
CDP-ethanolamine recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.53 Mn2+ required. Concentration of MnCl2 giving the half-maximal rate is 0.001-0.004 mM. Mn2+ cannot be replaced by Mg2+ Rattus norvegicus
3.6.1.53 Mn2+ dependent on, ADPRibase-Mn is inactive without added Mn2+, and it shows a high affinity for the cation, since saturation is observed at low-micromolar concentrations of Mn2+ well below the substrate concentration (0.5 mM), in no case can Mg2+ replace Mn2+ Rattus norvegicus
3.6.1.53 additional information Mg2+ cannot replace Mn2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.53 30000
-
gel filtration Rattus norvegicus
3.6.1.53 38000
-
SDS-PAGE Rattus norvegicus
3.6.1.53 39360
-
calculated from amino acid sequence Rattus norvegicus
3.6.1.53 40000
-
1 * 39360, calculated, 1 * 40000, SDS-PAGE, enzyme plus N-terminal extension GPLGSPNS left after proteolysis of recombinant fusion protein Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.53 Mus musculus Q99KS6
-
-
3.6.1.53 Rattus norvegicus Q5M886
-
-
3.6.1.53 Rattus norvegicus Q5M886 bifunctional Mn2+-dependent ADP-ribose/CDP-alcohol diphosphatase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.53 glutathione-Sepharose column chromatography Rattus norvegicus
3.6.1.53 recombinant GSt-fusion protein, in-column proteolysis of fusion Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.53 liver
-
Rattus norvegicus
-
3.6.1.53 additional information preferential expression in immune cells Mus musculus
-
3.6.1.53 skeletal muscle
-
Rattus norvegicus
-
3.6.1.53 spleen ADPRibase-Mn activity in spleen is 2.5-5fold higher than in liver and muscle Rattus norvegicus
-
3.6.1.53 splenocyte
-
Rattus norvegicus
-
3.6.1.53 thymus ADPRibase-Mn activity in thymus is 2.5-5fold higher than in liver and muscle Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.6.1.53 -20°C, 1 mM EDTA, stable for months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.53 ADP + H2O 27% activity at 0.5 mM substrate compared to ADP-ribose Rattus norvegicus ?
-
?
3.6.1.53 ADP + H2O 26% of the activity with ADP-ribose Rattus norvegicus AMP + phosphate
-
?
3.6.1.53 ADP-ribose + H2O
-
Rattus norvegicus AMP + D-ribose 5-phosphate
-
?
3.6.1.53 ADP-ribose + H2O 100% activity at 0.5 mM substrate Rattus norvegicus AMP + D-ribose 5-phosphate
-
?
3.6.1.53 CDP-choline + H2O 156% of the activity with ADP-ribose Rattus norvegicus CMP + phosphocholine
-
?
3.6.1.53 CDP-choline + H2O 140% activity at 0.5 mM substrate compared to ADP-ribose Rattus norvegicus CMP + phosphocholine
-
?
3.6.1.53 CDP-ethanolamine + H2O 84% of the activity with ADP-ribose Rattus norvegicus CMP + phosphoethanolamine
-
?
3.6.1.53 CDP-ethanolamine + H2O 89% activity at 0.5 mM substrate compared to ADP-ribose Rattus norvegicus ?
-
?
3.6.1.53 CDP-glycerol + H2O 103% of the activity with ADP-ribose Rattus norvegicus CMP + phosphoglycerol
-
?
3.6.1.53 CDP-glycerol + H2O 89% activity at 0.5 mM substrate compared to ADP-ribose Rattus norvegicus ?
-
?
3.6.1.53 additional information ADP-glucose, UDP-glucose, CDP-glucose, CDP, CMP, and AMP are not hydrolysed Rattus norvegicus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.6.1.53 monomer 1 * 39360, calculated, 1 * 40000, SDS-PAGE, enzyme plus N-terminal extension GPLGSPNS left after proteolysis of recombinant fusion protein Rattus norvegicus
3.6.1.53 monomer 1 * 30000, gel filtration Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.53 ADPRibase-I isozyme Rattus norvegicus
3.6.1.53 ADPRibase-II isozyme Rattus norvegicus
3.6.1.53 ADPRibase-Mn
-
Rattus norvegicus
3.6.1.53 Mn2+-dependent ADP-ribose/CDP-alcohol pyrophosphatase
-
Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.53 4.4
-
ADP pH 7.5, 37°C Rattus norvegicus
3.6.1.53 4.4
-
ADP recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C Rattus norvegicus
3.6.1.53 12.8
-
ADP-ribose pH 7.5, 37°C Rattus norvegicus
3.6.1.53 12.8
-
ADP-ribose recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 01 mM MnCl2, at 37°C Rattus norvegicus
3.6.1.53 28.1
-
CDP-glycerol pH 7.5, 37°C Rattus norvegicus
3.6.1.53 28.1
-
CDP-glycerol recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C Rattus norvegicus
3.6.1.53 28.5
-
CDP-choline pH 7.5, 37°C Rattus norvegicus
3.6.1.53 28.5
-
CDP-choline recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C Rattus norvegicus
3.6.1.53 41.3
-
CDP-ethanolamine pH 7.5, 37°C Rattus norvegicus
3.6.1.53 41.3
-
CDP-ethanolamine recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.53 6 8 activity towards ADP- and CDP-alcohols Rattus norvegicus
3.6.1.53 8 8.5
-
Rattus norvegicus
3.6.1.53 8 8.5 ADP-ribose hydrolase activity Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.53 6 10
-
Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.6.1.53 6 8 the activities towards ADP and CDP-alcohols are highest at pH 6-8 and decrease abruptly at higher pH Rattus norvegicus