Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Laco, G.S.; Pommier, Y.
    Role of a tryptophan anchor in human topoisomerase I structure, function and inhibition (2008), Biochem. J., 411, 523-530.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.1 expressed in insect cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.6.2.1 additional information plasmid constructs used: pTop65 has an initiator methionine codon followed by Top1 codons 214-765, pTop68 has an initiator methionine codon followed by six histidine codons and four codons encoding the Factor Xa protease-cleavage site, and then Top1 codons 201-765, pTop68W/A is the same as pTop68 except that Trp203, Trp205 and Trp206 are mutated to alanine, pTop69NLS has an initiator methionine codon followed by six histidine codons and four codons encoding the Factor Xa protease-cleavage site, and then Top1 codons 189-765 Homo sapiens
5.6.2.1 W203A/W205A/W206A lacking processivity, exhibits resistance to camptothecin and is inactivated by urea. Indicates that the tryptophan anchor stabilizes the N-terminus of the functional domain and revents the loss of Top1 structure and function Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.1 camptothecin inhibitory effect to wild-type Homo sapiens
5.6.2.1 Urea inhibitory effect to W203A/W205A/W206A mutant Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.1 Homo sapiens P11387
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.1 HiTrap Q HP column and SDS-PAGE Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.1 negatively supercoiled DNA
-
Homo sapiens relaxed DNA
-
?

Synonyms

EC Number Synonyms Comment Organism
5.6.2.1 human Top1
-
Homo sapiens
5.6.2.1 Topoisomerase I
-
Homo sapiens