Literature summary extracted from
Laco, G.S.; Pommier, Y.
Role of a tryptophan anchor in human topoisomerase I structure, function and inhibition (2008), Biochem. J., 411, 523-530.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.6.2.1 |
expressed in insect cells |
Homo sapiens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
5.6.2.1 |
additional information |
plasmid constructs used: pTop65 has an initiator methionine codon followed by Top1 codons 214-765, pTop68 has an initiator methionine codon followed by six histidine codons and four codons encoding the Factor Xa protease-cleavage site, and then Top1 codons 201-765, pTop68W/A is the same as pTop68 except that Trp203, Trp205 and Trp206 are mutated to alanine, pTop69NLS has an initiator methionine codon followed by six histidine codons and four codons encoding the Factor Xa protease-cleavage site, and then Top1 codons 189-765 |
Homo sapiens |
5.6.2.1 |
W203A/W205A/W206A |
lacking processivity, exhibits resistance to camptothecin and is inactivated by urea. Indicates that the tryptophan anchor stabilizes the N-terminus of the functional domain and revents the loss of Top1 structure and function |
Homo sapiens |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.6.2.1 |
camptothecin |
inhibitory effect to wild-type |
Homo sapiens |
|
5.6.2.1 |
Urea |
inhibitory effect to W203A/W205A/W206A mutant |
Homo sapiens |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.6.2.1 |
Homo sapiens |
P11387 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.6.2.1 |
HiTrap Q HP column and SDS-PAGE |
Homo sapiens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.6.2.1 |
negatively supercoiled DNA |
- |
Homo sapiens |
relaxed DNA |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.6.2.1 |
human Top1 |
- |
Homo sapiens |
5.6.2.1 |
Topoisomerase I |
- |
Homo sapiens |