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Literature summary extracted from
- Lipid phosphate phosphatases form homo- and hetero-oligomers: catalytic competency, subcellular distribution and function (2008), Biochem. J., 411, 371-377.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.4 | expression of Myc-tagged and/or FLAG-tagged LPP1, LLP2, and LLP3 in HEK-293 cells, overview | Mus musculus |
| 3.1.3.4 | LPP1 wild-type or mutants inserted into vector pcDNA3.1 with a C-terminal Myc epitope tag or FLAG epitope tag. HEK-293 cells transfected with plasmid construct encoding catalytically deficient Myc-tagged R127K LPP1 and/or FLAG-tagged H223L LPP1 and/or FLAG-tagged and/or Myc-tagged wild-type | Cavia porcellus |
| 3.1.3.4 | LPP2 and LPP3 inserted into vector pcDNA3.1 with a C-terminal Myc epitope tag or FLAG epitope tag and transiently transfected into HEK-293 cells | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.3.4 | H171L | site-directed mutagenesis of the C2 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
| 3.1.3.4 | H223L | site-directed mutagenesis of the C3 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
| 3.1.3.4 | H223L | mutated in C3 domain, catalytically deficient Myc-tagged R127K LPP1 and FLAG-tagged H223L LPP1 can form oligomers | Cavia porcellus |
| 3.1.3.4 | K120R | site-directed mutagenesis of the C1 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
| 3.1.3.4 | P128I | site-directed mutagenesis of the C1 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
| 3.1.3.4 | R127K | site-directed mutagenesis of the C1 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
| 3.1.3.4 | R127K | mutated in C1 domain, catalytically deficient Myc-tagged R127K LPP1 and FLAG-tagged H223L LPP1 can form oligomers | Cavia porcellus |
| 3.1.3.4 | R217K | site-directed mutagenesis of the C3 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
| 3.1.3.4 | S169T | site-directed mutagenesis of the C2 domain conserved amino acid residue leads to reduced activity of LPP1 | Mus musculus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.4 | additional information | Myc-gpLPP1-FLAG-LPP2, FLAG-gpLPP1-Myc-LPP3 and Myc-LPP2-FLAG-LPP3 are co-localized at the plasma membrane and in a perinuclear region of HEK-293 cells | Homo sapiens | - |
- |
| 3.1.3.4 | additional information | Myc-LPP1-FLAG-hLPP2 and FLAG-LPP1-Myc-hLPP3 are co-localized at the plasma membrane and in a perinuclear region of HEK-293 cells | Cavia porcellus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.4 | 31000 | - |
x * 31000 + x * 34500 + x * 35000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP1 | Cavia porcellus |
| 3.1.3.4 | 32000 | - |
x * 32000 + x * 34000, Western blot analysis, LPP3. X * 33000 , Western blot analysis, LPP2 | Cricetulus griseus |
| 3.1.3.4 | 32000 | - |
x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 | Homo sapiens |
| 3.1.3.4 | 33000 | - |
x * 32000 + x * 34000, Western blot analysis, LPP3. X * 33000 , Western blot analysis, LPP2 | Cricetulus griseus |
| 3.1.3.4 | 33000 | - |
x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 | Homo sapiens |
| 3.1.3.4 | 34000 | - |
x * 32000 + x * 34000, Western blot analysis, LPP3. X * 33000 , Western blot analysis, LPP2 | Cricetulus griseus |
| 3.1.3.4 | 34000 | - |
x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 | Homo sapiens |
| 3.1.3.4 | 34500 | - |
x * 31000 + x * 34500 + x * 35000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP1 | Cavia porcellus |
| 3.1.3.4 | 35000 | - |
x * 31000 + x * 34500 + x * 35000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP1 | Cavia porcellus |
| 3.1.3.4 | 36000 | - |
x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.4 | Cavia porcellus | - |
- |
- |
| 3.1.3.4 | Cricetulus griseus | - |
- |
- |
| 3.1.3.4 | Homo sapiens | - |
- |
- |
| 3.1.3.4 | Mus musculus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.4 | CHO cell | - |
Cricetulus griseus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.4 | additional information | endogenous LPP2 and LPP3 form a complex | Mus musculus | ? | - |
? |  |
| 3.1.3.4 | phosphatidic acid + H2O | - |
Cavia porcellus | 1,2-diacyl-sn-glycerol + phosphate | - |
? | |
| 3.1.3.4 | phosphatidic acid + H2O | - |
Homo sapiens | 1,2-diacyl-sn-glycerol + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.4 | ? | x * 31000-36000, recombinant Myc-tagged and/or FLAG-tagged LPP1 or LLP3, SDS-PAGE | Mus musculus |
| 3.1.3.4 | ? | x * 31000 + x * 34500 + x * 35000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP1 | Cavia porcellus |
| 3.1.3.4 | ? | x * 32000 + x * 34000, Western blot analysis, LPP3. X * 33000 , Western blot analysis, LPP2 | Cricetulus griseus |
| 3.1.3.4 | ? | x * 33000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP2. x * 32000 + x * 34000 + x * 36000, SDS-PAGE, FLAG-tagged and Myc-tagged LPP3 | Homo sapiens |
| 3.1.3.4 | More | LPP1 monomers might exist in equilibrium with oligomeric LPP1, the LPP1 monomer is inactive, endogenous LPP2 and LPP3 form a complex | Mus musculus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.4 | lipid phosphate phosphatase | - |
Cricetulus griseus |
| 3.1.3.4 | lipid phosphate phosphatase | - |
Cavia porcellus |
| 3.1.3.4 | lipid phosphate phosphatase | - |
Mus musculus |
| 3.1.3.4 | lipid phosphate phosphatase | - |
Homo sapiens |
| 3.1.3.4 | LLP2 | - |
Mus musculus |
| 3.1.3.4 | LPP1 | - |
Cavia porcellus |
| 3.1.3.4 | LPP1 | - |
Mus musculus |
| 3.1.3.4 | LPP2 | - |
Cricetulus griseus |
| 3.1.3.4 | LPP2 | - |
Homo sapiens |
| 3.1.3.4 | LPP3 | - |
Cricetulus griseus |
| 3.1.3.4 | LPP3 | - |
Mus musculus |
| 3.1.3.4 | LPP3 | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.4 | 30 | - |
assay at | Mus musculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.4 | 7 | - |
assay at | Mus musculus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.3.4 | malfunction | catalytically deficient FLAG-tagged H223L LPP1 mutant can form an oligomer with wild-type LPP1, whereby wild-type LPP1 activity is preserved in the oligomer | Cavia porcellus |
| 3.1.3.4 | physiological function | endogenous LPP2 and LPP3 form a complex. Endogenous LPP2 and LPP3 form homo- and hetero-oligomers, which differ in their subcellular localization and which may confer differing spatial regulation of phosphatidic acid and sphingosine 1-phosphate signalling | Homo sapiens |
| 3.1.3.4 | physiological function | LPP1 forms both homo- and hetero-oligomers. Full catalytic activity is not required for oligomerization | Cavia porcellus |
| 3.1.3.4 | physiological function | LPP2 forms a complex with the 32 kDa form of LPP3, but not with the 34 kDa form. LPP oligomers may regulate compartmentalized pools of sphingosine 1-phosphate and phosphatidic acid and contribute to the spatial signalling by these lipids within cells | Cricetulus griseus |
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