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Literature summary extracted from

  • Cho, E.Y.; Yun, C.H.; Chae, H.Z.; Chae, H.J.; Ahn, T.
    Lysophosphatidylserine-induced functional switch of human cytochrome P450 1A2 and 2E1 from monooxygenase to phospholipase D (2008), Biochem. Biophys. Res. Commun., 376, 584-589.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 lysophosphatidylserine stimulates PLD activity in a concentration-dependent manner and approximately 5fold and 8fold increases in CYP1A2 and CYP2E1 activities, respectively, are shown in the presence of 2 mol% of the lysophosphatidylserine when compared to a 100% phosphatidylcholine matrix. LysoPS also accompanies conformational changes in both CYP1A2 and CYP2E1 when assayed by circular dichroism Homo sapiens
3.1.4.4 lysophosphatidylserine stimulates PLD activity in a concentration-dependent manner and approximately 5fold and 8fold increases in CYP1A2 and CYP2E1 activities, respectively, are shown in the presence of 2 mol% of the lysophosphatidylserine when compared to a 100% phosphatidylcholine matrix. LysoPS also accompanies conformational changes in both CYP1A2 and CYP2E1 when assayed by circular dichroism Rattus norvegicus

Application

EC Number Application Comment Organism
3.1.4.4 molecular biology functional roles of CYP1A2 and CYP2E1 can be switched by interaction with a specific anionic lysophospholipid in vivo Homo sapiens
3.1.4.4 molecular biology functional roles of CYP1A2 and CYP2E1 can be switched by interaction with a specific anionic lysophospholipid in vivo Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4 expressed in Escherichia coli Homo sapiens
3.1.4.4 expressed in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 additional information PLD activity is decreased in the presence of components required for the monooxygenase (MMO) activity (reducing system), including 100% phosphatidylcholine membranes, NADPH-cytochrome P450 reductase and NADPH. Lysophosphatidylserine recoveres the PLD activity in a concentration-dependent manner Homo sapiens
3.1.4.4 additional information PLD activity is decreased in the presence of components required for the monooxygenase (MMO) activity (reducing system), including 100% phosphatidylcholine membranes, NADPH-cytochrome P450 reductase and NADPH. Lysophosphatidylserine recoveres the PLD activity in a concentration-dependent manner Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Homo sapiens
-
-
-
3.1.4.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 using using Ni2+-affinity and ion-exchange chromatography Homo sapiens
3.1.4.4 using using Ni2+-affinity and ion-exchange chromatography Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 phosphatidylcholine + H2O
-
 Homo sapiens choline + phosphatidate
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Rattus norvegicus choline + phosphatidate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 phospholipase D
-
 Homo sapiens
3.1.4.4 phospholipase D
-
 Rattus norvegicus
3.1.4.4 PLD
-
 Homo sapiens
3.1.4.4 PLD
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.4 37
-
 assay at Homo sapiens
3.1.4.4 37
-
 assay at Rattus norvegicus