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Literature summary extracted from

  • Fukunaga, R.; Harada, Y.; Hirao, I.; Yokoyama, S.
    Phosphoserine aminoacylation of tRNA bearing an unnatural base anticodon (2008), Biochem. Biophys. Res. Commun., 372, 480-485.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.27 D418N/D420N/T423V site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme, it is active with tRNA substrate containing unusual residues 7-(2-thienyl)-imidazo[4,5-b]pyridine and pyrrole-2-carbaldehyde in the anticodon, overview Archaeoglobus fulgidus
6.1.1.27 E418N/E420N/T423V efficiently charged phosphoserine to tRNA containing the (pyrrole-2-carbaldehyde)UA anticodon Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.27 additional information
-
 additional information kinetic analysis of Sep-tRNA formation, overview Archaeoglobus fulgidus
6.1.1.27 0.0269
-
 tRNACys pH 7.6, 50°C, wild-type enzyme Archaeoglobus fulgidus
6.1.1.27 0.0372
-
 tRNACys pH 7.6, 50°C, mutant D418N/D420N/T423V Archaeoglobus fulgidus
6.1.1.27 0.151
-
 tRNACys 50°C, pH 7.6, tRNACys containing the (pyrrole-2-carbaldehyde)UA anticodon, mutant enzyme D418N D420N T423V Archaeoglobus fulgidus
6.1.1.27 0.151
-
 tRNACys 50°C, pH 7.6, tRNACys containing the C(pyrrole-2-carbaldehyde)A anticodon, mutant enzyme D418N D420N T423V Archaeoglobus fulgidus
6.1.1.27 0.269
-
 tRNACys 50°C, pH 7.6, tRNACys with the GCA anticodon, wild-type enzyme Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.27 Mg2+
-
 Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.27 ATP + O-phospho-L-serine + tRNACys Archaeoglobus fulgidus
-
 AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?
6.1.1.27 ATP + O-phospho-L-serine + tRNACys Archaeoglobus fulgidus phosphoseryl-tRNA synthetase is a natural non-standard aminoacyl-tRNA synthetase, which charges a non-standard amino acid, phosphoserine, to tRNACys containing a GCA anticodon for tRNA-dependent cysteine biosynthesis in some archaea AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.27 Archaeoglobus fulgidus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.27 ATP + O-phospho-L-serine + tRNAAmber mutant D418N/D420N/T423V Archaeoglobus fulgidus AMP + diphosphate + O-phospho-L-seryl-tRNAAmber
-
 ?
6.1.1.27 ATP + O-phospho-L-serine + tRNACys
-
 Archaeoglobus fulgidus AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?
6.1.1.27 ATP + O-phospho-L-serine + tRNACys phosphoseryl-tRNA synthetase is a natural non-standard aminoacyl-tRNA synthetase, which charges a non-standard amino acid, phosphoserine, to tRNACys containing a GCA anticodon for tRNA-dependent cysteine biosynthesis in some archaea Archaeoglobus fulgidus AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?
6.1.1.27 ATP + O-phospho-L-serine + tRNACys cognate substrate is tRNACys with the GCA anticodon, tRNACys containing the (pyrrole-2-carbaldehyde)UA anticodon Archaeoglobus fulgidus AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?
6.1.1.27 ATP + O-phospho-L-serine + tRNAOpal mutant D418N/D420N/T423V Archaeoglobus fulgidus AMP + diphosphate + O-phospho-L-seryl-tRNAOpal
-
 ?
6.1.1.27 additional information substrate specificity, site-specific incorporation of phosphoserine into proteins by mutant D418N/D420N/T423V in response to the 7-(2-thienyl)-imidazo[4,5-b]pyridineUA or C7-(2-thienyl)-imidazo[4,5-b]pyridineA codons within mRNA, substrate binding structures and structural models for tRNA anticodon recognition, overview Archaeoglobus fulgidus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.27 phosphoseryl-tRNA synthetase
-
 Archaeoglobus fulgidus
6.1.1.27 phosphoseryl-tRNA synthetases
-
 Archaeoglobus fulgidus
6.1.1.27 SepRS
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.27 50
-
 assay at Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.27 0.008
-
 tRNACys pH 7.6, 50°C, mutant D418N/D420N/T423V Archaeoglobus fulgidus
6.1.1.27 0.0471
-
 tRNACys 50°C, pH 7.6, tRNACys containing the (pyrrole-2-carbaldehyde)UA anticodon, mutant enzyme D418N D420N T423V Archaeoglobus fulgidus
6.1.1.27 0.048
-
 tRNACys 50°C, pH 7.6, tRNACys containing the C(pyrrole-2-carbaldehyde)A anticodon, mutant enzyme D418N D420N T423V Archaeoglobus fulgidus
6.1.1.27 0.115
-
 tRNACys 50°C, pH 7.6, tRNACys with the GCA anticodon, wild-type enzyme Archaeoglobus fulgidus
6.1.1.27 0.115
-
 tRNACys pH 7.6, 50°C, wild-type enzyme Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.27 7.5
-
 assay at Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.27 ATP
-
 Archaeoglobus fulgidus