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Literature summary extracted from

  • Prasannan, P.; Appling, D.R.
    Human mitochondrial C(1)-tetrahydrofolate synthase: Submitochondrial localization of the full-length enzyme and characterization of a short isoform (2008), Arch. Biochem. Biophys., 481, 86-93.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.3 expressed in CHO cells and Escherichia coli Homo sapiens
6.3.4.3 expressed in CHO cells and Escherichia coli Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.3 additional information the recombinant protein product (short isoform) of the alternatively spliced short transcript of the mitochondrial isozyme is not enzymatically active Homo sapiens
6.3.4.3 additional information the recombinant protein product (short isoform) of the alternatively spliced short transcript of the mitochondrial isozyme is not enzymatically active Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.4.3 mitochondrion mitochondrial C1-THF synthase behaves as a peripheral membrane protein, tightly associated with the matrix side of the mitochondrial inner membrane Homo sapiens 5739
-
6.3.4.3 mitochondrion mitochondrial C1-THF synthase behaves as a peripheral membrane protein, tightly associated with the matrix side of the mitochondrial inner membrane Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.3 35000
-
 recombinant short isoform, SDS-PAGE Homo sapiens
6.3.4.3 107000
-
 recombinant mitochondrial C1-THF synthase without the presequence but with the V5 epitope tag, SDS-PAGE Homo sapiens
6.3.4.3 107000
-
 recombinant mitochondrial C1-THF synthase without the presequence but with the V5 epitope tag, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.3 Homo sapiens
-
-
-
6.3.4.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.3 TALON cobalt metal affinity resin chromatography Homo sapiens
6.3.4.3 TALON cobalt metal affinity resin chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.3 placenta
-
 Homo sapiens
-
6.3.4.3 spleen
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Homo sapiens ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Rattus norvegicus ADP + phosphate + 10-formyltetrahydrofolate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.3 C(1)-tetrahydrofolate synthase a monofunctional 10-formyl-tetrahydrofolate synthetase lacking the 5,10-methylene-tetrahydrofolate dehydrogenase and 5,10-methenyl-tetrahydrofolate cyclohydrolase activities typically found in the trifunctional cytoplasmic proteins Homo sapiens
6.3.4.3 C(1)-tetrahydrofolate synthase a monofunctional 10-formyl-tetrahydrofolate synthetase lacking the 5,10-methylene-tetrahydrofolate dehydrogenase and 5,10-methenyl-tetrahydrofolate cyclohydrolase activities typically found in the trifunctional cytoplasmic proteins Rattus norvegicus
6.3.4.3 MTHFDIL
-
 Homo sapiens
6.3.4.3 MTHFDIL
-
 Rattus norvegicus
6.3.4.3 THF synthase
-
 Homo sapiens
6.3.4.3 THF synthase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.3 ATP
-
 Homo sapiens
6.3.4.3 ATP
-
 Rattus norvegicus