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Literature summary extracted from

  • Pendini, N.R.; Bailey, L.M.; Booker, G.W.; Wilce, M.C.; Wallace, J.C.; Polyak, S.W.
    Biotin protein ligase from Candida albicans: expression, purification and development of a novel assay (2008), Arch. Biochem. Biophys., 479, 163-169.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.4.15 drug development the enzyme is a target for the design of effective antifungal drugs Candida albicans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.15 expression of His9-tagged BPL in Saccharomyces cerevisiae strain w303, functional complementation of the conditional lethal birA85 Escherichia coli strain BM4062 Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.15 additional information disruption of BPL affects important metabolic pathways such as fatty acid biosynthesis and gluconeogenesis Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.4.15 cytoplasm
-
 Candida albicans 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.15 Mg2+
-
 Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.15 73000
-
 x * 73000, recombinant His9-tagged BPL, SDS-PAGE Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Candida albicans BPL is an essential enzyme responsible for the activation of biotin-dependent enzymes through the covalent attachment of biotin AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Candida albicans CBF562 BPL is an essential enzyme responsible for the activation of biotin-dependent enzymes through the covalent attachment of biotin AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Candida albicans
-
-
-
6.3.4.15 Candida albicans CBF562
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.15 recombinant His9-tagged BPL from Saccharomyces cerevisiae strain w303 by nickel affinity and cation exchange chromatography Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.15 0.049
-
 purified recombinant enzyme Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] BPL is an essential enzyme responsible for the activation of biotin-dependent enzymes through the covalent attachment of biotin Candida albicans AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] determination of the biotin domains of acetyl-CoA carboxylase, ACC Candida albicans AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] determination of the biotin domains of the pyruvate carboxylase, PC, of the organism, substrate is peptide fragment CaPC115 as GST-tagged recombinant protein, overview Candida albicans AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] BPL is an essential enzyme responsible for the activation of biotin-dependent enzymes through the covalent attachment of biotin Candida albicans CBF562 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] determination of the biotin domains of acetyl-CoA carboxylase, ACC Candida albicans CBF562 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] determination of the biotin domains of the pyruvate carboxylase, PC, of the organism, substrate is peptide fragment CaPC115 as GST-tagged recombinant protein, overview Candida albicans CBF562 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.15 ? x * 73000, recombinant His9-tagged BPL, SDS-PAGE Candida albicans

Synonyms

EC Number Synonyms Comment Organism
6.3.4.15 biotin protein ligase
-
 Candida albicans
6.3.4.15 BPL
-
 Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.15 37
-
 assay at Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.15 8
-
 assay at Candida albicans

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.15 ATP
-
 Candida albicans