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Literature summary extracted from

  • Papaneophytou, C.P.; Pantazaki, A.A.; Kyriakidis, D.A.
    An extracellular polyhydroxybutyrate depolymerase in Thermus thermophilus HB8 (2009), Appl. Microbiol. Biotechnol., 83, 659-668.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.75 acetone 10% (v/v): 102% activity compared to no addition Thermus thermophilus HB8
3.1.1.75 ethanol 10% (v/v): 100% activity compared to no addition Thermus thermophilus HB8
3.1.1.75 methanol 10% (v/v): 137% activity compared to no addition Thermus thermophilus HB8

Application

EC Number Application Comment Organism
3.1.1.75 industry extracellular polyhydroxybutyrate depolymerase is an excellent thermostable candidate biocatalyst for environmental, industrial, and medical applications Thermus thermophilus HB8
3.1.1.75 medicine extracellular polyhydroxybutyrate depolymerase is an excellent thermostable candidate biocatalyst for environmental, industrial, and medical applications Thermus thermophilus HB8

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.75 2-mercaptoethanol 1% (v/v): 20% activity, compared to no addition Thermus thermophilus HB8
3.1.1.75 EDTA 0.1 mM: 51%, 1 mM: 24%, 10 mM: 17% activity, compared to no addition Thermus thermophilus HB8
3.1.1.75 NaCl 1 mM: 100%, 2 mM: 90%, 5 mM: 60%, 10 mM: 45% activity, compared to no addition Thermus thermophilus HB8
3.1.1.75 NaN3 1 mM: 86%, 10 mM: 84% activity, compared to no addition Thermus thermophilus HB8
3.1.1.75 PMSF 1 mM: 24%, 10 mM: 10% activity, compared to no addition Thermus thermophilus HB8
3.1.1.75 Triton X-100 0.1% (v/v): 115%, 1% (v/v): 70%, 10% (v/v): 56% activity, compared to no addition Thermus thermophilus HB8
3.1.1.75 Tween 20 0.1% (v/v): 100%, 1% (v/v): 64%, 10% (v/v): 25% activity, compared to no addition Thermus thermophilus HB8
3.1.1.75 Tween 80 0.1% (v/v): 80%, 1% (v/v): 65%, 10% (v/v): 42% activity, compared to no addition Thermus thermophilus HB8

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.75 additional information
-
 additional information apparent Km value of the purified enzyme for polyhydroxybutyrate is 53 microg/ml Thermus thermophilus HB8

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Thermus thermophilus HB8
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.75 CaCl2 1 mM: 150%, 2 mM: 206%, 5 mM: 145%, 10 mM: 90% activity Thermus thermophilus HB8
3.1.1.75 KCl 1 mM: 140%, 2 mM: 150%, 5 mM: 100%, 10 mM: 80% activity Thermus thermophilus HB8
3.1.1.75 MgCl2 1 mM: 120%, 2 mM: 90%, 5 mM: 55%, 10 mM: 30% activity Thermus thermophilus HB8

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.75 42000
-
-
 Thermus thermophilus HB8

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Thermus thermophilus HB8
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75 affinity chromatography Thermus thermophilus HB8

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.75 56.3
-
 purification step: affinity binding to polyhydroxybutyrate Thermus thermophilus HB8

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 4-nitrophenyl butyrate + H2O
-
 Thermus thermophilus HB8 4-nitrophenol + butyrate
-
 ?
3.1.1.75 additional information purified enzyme is able to hydrolyze 4-nitrophenyl butyrate but not other artificial p-nitrophenyl fatty acid esters, such as p-nitrophenyl octanoate, p-nitrophenyl laurate, indicating preference of this enzyme for C4 substrates Thermus thermophilus HB8 ?
-
 ?
3.1.1.75 polyhydroxybutyrate + H2O
-
 Thermus thermophilus HB8 3-hydroxybutyrate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.75 PHB depolymerase
-
 Thermus thermophilus HB8
3.1.1.75 polyhydroxybutyrate depolymerase
-
 Thermus thermophilus HB8

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.75 70
-
 optimum temperature of the polyhydroxybutyrate depolymerase enzymic reaction, using polyhydroxybutyrate as substrate, is 70°C Thermus thermophilus HB8

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.75 additional information
-
 stable at high temperatures Thermus thermophilus HB8

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.75 8
-
-
 Thermus thermophilus HB8