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Literature summary extracted from

  • Basile, L.J.; Willson, R.C.; Sewell, B.T.; Benedik, M.J.
    Genome mining of cyanide-degrading nitrilases from filamentous fungi (2008), Appl. Microbiol. Biotechnol., 80, 427-435.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.66 cyanide expression induced in presence of Aspergillus nidulans
4.2.1.66 cyanide expression induced in presence of Neurospora crassa

Application

EC Number Application Comment Organism
4.2.1.66 biotechnology searching for enzymes in the bioremediation of cyanide-containing waste Aspergillus nidulans
4.2.1.66 biotechnology searching for enzymes in the bioremediation of cyanide-containing waste Neurospora crassa
4.2.1.66 biotechnology searching for enzymes in the bioremediation of cyanide-containing waste Fusarium graminearum
4.2.1.66 biotechnology searching for enzymes in the bioremediation of cyanide-containing waste Microdochium sorghi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.66 expressed in Escherichia coli, strains and plasmids listed Aspergillus nidulans
4.2.1.66 expressed in Escherichia coli, strains and plasmids listed Neurospora crassa
4.2.1.66 expressed in Escherichia coli, strains and plasmids listed Fusarium graminearum
4.2.1.66 expressed in Escherichia coli, strains and plasmids listed Microdochium sorghi

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.66 additional information rate of the reaction decreases at high substrate concentrations, indicative of substrate inhibition Aspergillus nidulans
4.2.1.66 additional information rate of the reaction decreases at high substrate concentrations, indicative of substrate inhibition Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.66 HCN + H2O Aspergillus nidulans
-
 formamide
-
 ?
4.2.1.66 HCN + H2O Neurospora crassa
-
 formamide
-
 ?
4.2.1.66 HCN + H2O Fusarium graminearum
-
 formamide
-
 ?
4.2.1.66 HCN + H2O Microdochium sorghi
-
 formamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.66 Aspergillus nidulans
-
-
-
4.2.1.66 Fusarium graminearum
-
-
-
4.2.1.66 Microdochium sorghi P32964
-
-
4.2.1.66 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.66 recombinant protein, gel filtration Aspergillus nidulans
4.2.1.66 recombinant protein, gel filtration Neurospora crassa
4.2.1.66 recombinant protein, gel filtration Fusarium graminearum
4.2.1.66 recombinant protein, gel filtration Microdochium sorghi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.66 additional information
-
 comparison of fungal nitrilases to determine best candidates for the remediation of cyanide-containing industrial wastewater, analysis according to relative specific activity, pH activity profile, thermal stability, and ability to remediate cyanide contaminated waste water from silver and copper electroplating baths Fusarium graminearum
4.2.1.66 additional information
-
 comparison of fungal nitrilases to determine best candidates for the remediation of cyanide-containing industrial wastewater, enzyme of Aspergillus nidulans indicates highest reaction rate of the four fungal nitrilases compared in the study Aspergillus nidulans
4.2.1.66 additional information
-
 comparison of fungal nitrilases to determine best candidates for the remediation of cyanide-containing industrial wastewater, lowest activity of the group of nitrilases compared in this study, does not reach maximum activity until 80 mM KCN Microdochium sorghi
4.2.1.66 additional information
-
 comparison of fungal nitrilases to determine best candidates for the remediation of cyanide-containing industrial wastewater, most promising candidate isolated from Neurospora crassa, according to high activity over a wide range of pH values, stability for at least 48 h when incubated at temperatures ranging from 27°C to 43°C, highest rates at a substrate concentration of 20 mM KCN, maximal rates around 60 mM KCN Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.66 HCN + H2O
-
 Aspergillus nidulans formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Neurospora crassa formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Fusarium graminearum formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Microdochium sorghi formamide
-
 ?
4.2.1.66 HCN + H2O emediation of cyanide-containing industrial wastewater analyzed Aspergillus nidulans formamide
-
 ?
4.2.1.66 HCN + H2O emediation of cyanide-containing industrial wastewater analyzed Neurospora crassa formamide
-
 ?
4.2.1.66 HCN + H2O emediation of cyanide-containing industrial wastewater analyzed Fusarium graminearum formamide
-
 ?
4.2.1.66 HCN + H2O emediation of cyanide-containing industrial wastewater analyzed Microdochium sorghi formamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.66 CHT
-
 Aspergillus nidulans
4.2.1.66 CHT
-
 Neurospora crassa
4.2.1.66 CHT
-
 Fusarium graminearum
4.2.1.66 CHT
-
 Microdochium sorghi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.66 27 50 incubated at 27°C, 37°C, 43°C, and 50°C, activity measured at time points ranging between 0 and 48 h, at 37°C and 43°C most stable with no loss of activity after 48 h at 37°C, about 40% of activity remaining after 48 h at 43°C, at 50°C activity below 5% by 48 h Neurospora crassa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.66 27 50 incubated at 27°C, 37°C, 43°C, and 50°C, activity measured at time points ranging between 0 and 48 h, CHT of Gibberella zeae shows an initial increase in activity at lower temperatures, subsequent stability is similar to that of Neurospora crassa, gradual decrease in activity at 50°C Fusarium graminearum
4.2.1.66 27 50 incubated at 27°C, 37°C, 43°C, and 50°C, activity measured at time points ranging between 0 and 48 h, enzyme of Aspergillus nidulans quickly looses activity at the two highest temperatures, and 50% of activity is lost after 48 h at 37°C Aspergillus nidulans
4.2.1.66 27 50 incubated at 27°C, 37°C, 43°C, and 50°C, activity measured at time points ranging between 0 and 48 h, enzyme quickly looses activity at the two highest temperatures, 50% of activity is lost after 48 h at 37°C Microdochium sorghi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.66 5.2 9 at least half-maximal activity between pH 5.2 and pH 9, widest range of activity in comparison to four other enzymes used in this study Neurospora crassa
4.2.1.66 5.2 9 relatively wide range of >50% activity relative to its maximal rate Aspergillus nidulans
4.2.1.66 6 8.5 at least half-maximal activity between approximately pH 6 and pH 8.5, narrower range of activity in comparison to four other enzymes used in this study Fusarium graminearum
4.2.1.66 6 8.5 narrow range of activity in comparison to four other enzymes used in this study, more tolerant of higher than of lower pH values Microdochium sorghi