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Literature summary extracted from

  • Sousa, S.A.; Moreira, L.M.; Leitao, J.H.
    Functional analysis of the Burkholderia cenocepacia J2315 BceAJ protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities (2008), Appl. Microbiol. Biotechnol., 80, 1015-1022.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.1.8 Ca2+ order of activation Mg2+ > Ca2+ > Mn2+ > Co2+ > Ni2+ Burkholderia cenocepacia
5.3.1.8 Co2+ order of activation Mg2+ > Ca2+ > Mn2+ > Co2+ > Ni2+ Burkholderia cenocepacia
5.3.1.8 Mg2+ order of activation Mg2+ > Ca2+ > Mn2+ > Co2+ > Ni2+ Burkholderia cenocepacia
5.3.1.8 Mn2+ Mg2+ > Ca2+ > Mn2+ > Co2+ > Ni2+ Burkholderia cenocepacia
5.3.1.8 Ni2+ order of activation Mg2+ > Ca2+ > Mn2+ > Co2+ > Ni2+ Burkholderia cenocepacia

Application

EC Number Application Comment Organism
2.7.7.13 drug development the enzyme is a potential target for antimicrobial drug design, i.e. in the treatment of cystic fibrosis caused by Burkholderia cenocepacia Burkholderia cenocepacia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.13 gene bceA, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Burkholderia cenocepacia
5.3.1.8 expression in Escherichia coli Burkholderia cenocepacia

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.8 Zn2+ inhibitory to both phosphomannose isomerase and guanosine diphosphate-D-mannose diphosphorylase activities Burkholderia cenocepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 0.024
-
 GDP-mannose pH 7.6, recombinant enzyme, in presence of 5 mM Mg2+ and 2 mM diphosphate Burkholderia cenocepacia
5.3.1.8 12.4
-
 D-mannose 6-phosphate 30°C, pH 7.6 Burkholderia cenocepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.13 Ca2+ GMP activity is strongly dependent on the presence of Ca2+ or Mn2+ Burkholderia cenocepacia
2.7.7.13 Co2+ activates slightly Burkholderia cenocepacia
2.7.7.13 Mg2+ activates Burkholderia cenocepacia
2.7.7.13 Mn2+ GMP activity is strongly dependent on the presence of Ca2+ or Mn2+, the presence of 5 mM Mn2+, precipitation occurred in the reaction mixture Burkholderia cenocepacia
2.7.7.13 additional information descending order of activation Mg2+, Ca2+, Mn2+, Co2+, Ni2+ Burkholderia cenocepacia
2.7.7.13 Ni2+ activates slightly Burkholderia cenocepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.13 55716
-
 x * 56000, recombinant enzyme, SDS-PAGE, x * 55716, sequence caculation Burkholderia cenocepacia
2.7.7.13 56000
-
 x * 56000, recombinant enzyme, SDS-PAGE, x * 55716, sequence caculation Burkholderia cenocepacia
5.3.1.8 55716
-
 x * 55716, calculated, x * 56000, SDS-PAGE Burkholderia cenocepacia
5.3.1.8 56000
-
 x * 55716, calculated, x * 56000, SDS-PAGE Burkholderia cenocepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.13 diphosphate + GDP-mannose Burkholderia cenocepacia
-
 GTP + alpha-D-mannose 1-phosphate
-
 ?
2.7.7.13 additional information Burkholderia cenocepacia the enzyme of Burkholderia cenocepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Burkholderia cenocepacia B4EMQ6 gene bceA
-
5.3.1.8 Burkholderia cenocepacia
-
 bifunctional enzyme with phosphomannose isomerase and guanosine diphosphate-D-mannose diphosphorylase activities
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity Burkholderia cenocepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 diphosphate + GDP-mannose
-
 Burkholderia cenocepacia GTP + alpha-D-mannose 1-phosphate
-
 ?
2.7.7.13 additional information the enzyme of Burkholderia cenocepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities Burkholderia cenocepacia ?
-
 ?
5.3.1.8 D-Mannose 6-phosphate
-
 Burkholderia cenocepacia D-Fructose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.13 ? x * 56000, recombinant enzyme, SDS-PAGE, x * 55716, sequence caculation Burkholderia cenocepacia
2.7.7.13 More the enzyme's amino acid sequence contains several conserved motifs: 1. the pyrophosphorylase signature sequence 38GGXGXRLXPLX5PK54, 2. the GMP active site 210FVEKP214, 3. the zinc-binding motif 436QXH438 and the putative PMI active site 465EN(Q/E)SX (Y/F)I471 Burkholderia cenocepacia
5.3.1.8 ? x * 55716, calculated, x * 56000, SDS-PAGE Burkholderia cenocepacia

Synonyms

EC Number Synonyms Comment Organism
2.7.7.13 BceA(J) protein
-
 Burkholderia cenocepacia
2.7.7.13 BceAJ
-
 Burkholderia cenocepacia
2.7.7.13 GDP-D-mannose pyrophosphorylase
-
 Burkholderia cenocepacia
5.3.1.8 BceAJ
-
 Burkholderia cenocepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.13 7.6
-
 assay at Burkholderia cenocepacia

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.1.8 Burkholderia cenocepacia calculated
-
 5.9